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PDBsum entry 1v7b
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Gene reguration
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PDB id
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1v7b
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References listed in PDB file
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Key reference
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Title
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The cgl2612 protein from corynebacterium glutamicum is a drug resistance-Related transcriptional repressor: structural and functional analysis of a newly identified transcription factor from genomic DNA analysis.
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Authors
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H.Itou,
U.Okada,
H.Suzuki,
M.Yao,
M.Wachi,
N.Watanabe,
I.Tanaka.
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Ref.
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J Biol Chem, 2005,
280,
38711-38719.
[DOI no: ]
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PubMed id
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Abstract
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The emergence of antibiotic-resistant bacteria often causes serious clinical
problems. The TetR family is one of the major transcription factor families that
regulate expression of genes involved in bacterial antimicrobial resistance
systems. CGL2612 protein is a transcription factor newly identified by genomic
DNA analysis on Corynebacterium glutamicum, which belongs to the mycolic
acid-containing Actinomycetales, including the well known pathogens
Corynebacterium diphtheriae and Mycobacterium tuberculosis. Crystal structure
analysis showed that the CGL2612 protein exhibits significant structural
similarity to the multidrug resistance (MDR)-related transcription factor QacR
from Staphylococcus aureus, despite poor amino acid sequence similarity between
these proteins. Binding DNA sequence analysis of CGL2612 protein using the
systematic evolution of ligands by the exponential enrichment (systematic
evolution of ligands by exponential enrichment, or SELEX) method revealed that
this protein is a new member of the TetR family, which regulates expression of
the immediately upstream gene, cgl2611, probably encoding a major facilitator
superfamily permease. Subsequent functional analyses confirmed a function of the
CGL2612 as a transcriptional repressor responsible for the antimicrobial
resistance system in C. glutamicum. The strategy used in the present study is
one of the most convenient and powerful methods to analyze functionally unknown
transcription factors, and the results obtained here will contribute to our
understanding of the drug resistance mechanism not only in C. glutamicum but
also in the related bacteria, C. diphtheriae and M. tuberculosis.
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Figure 1.
FIGURE 1. Ribbon representation of CGL2612 dimer. Each
subunit is shown in gray and white. Ribbon representations
appearing in this figure and Fig. 5b were generated using the
MOLSCRIPT (30) and RASTER3D (31) programs.
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Figure 4.
FIGURE 4. Top, schematic representation of the structures
of cgl2611 and cgl2612 genes and the cgl2611 promoter region.
Bottom, sequence representation of the DNA fragments isolated by
genomic and random SELEX analysis. In genomic SELEX, the
consensus sequence composed of all isolated fragments is
represented in boldface type. In random SELEX, positions
homologous to the consensus sequence are highlighted in boldface
type. The opposing two arrows indicate the half-site of the
pseudoinverted-repeat sequence observed here. *, the symmetry
center of the palindrome.
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The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2005,
280,
38711-38719)
copyright 2005.
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