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UniProt functional annotation for O59543 | ||
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| UniProt code: O59543. |
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| Organism: | |
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus. |
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| Function: | |
Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Not absolutely essential for activity in vitro, however it strongly stimulates activity. Binds RNase P RNA. {ECO:0000255|HAMAP-Rule:MF_00756, ECO:0000269|PubMed:12810070, ECO:0000269|PubMed:16574071, ECO:0000269|PubMed:16829535}. |
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| Catalytic activity: | |
Reaction=Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.; EC=3.1.26.5; Evidence={ECO:0000255|HAMAP- Rule:MF_00756}; |
| Biophysicochemical properties: | |
Temperature dependence: Optimum temperature is 70 degrees Celsius. {ECO:0000269|PubMed:12810070, ECO:0000269|PubMed:16574071}; |
| Subunit: | |
Consists of a catalytic RNA component and at least 5 protein subunits. Forms a heterotetrameric subcomplex with Rnp2. Reconstituted enzyme missing individual protein subunits is suboptimally active, showing each subunit contributes to optimization of activity. {ECO:0000269|PubMed:12810070, ECO:0000269|PubMed:16430919, ECO:0000269|PubMed:16574071, ECO:0000269|PubMed:16829535}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00756}. |
| Similarity: | |
Belongs to the eukaryotic/archaeal RNase P protein component 3 family. {ECO:0000255|HAMAP-Rule:MF_00756}. |
Annotations taken from UniProtKB at the EBI.