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PDBsum entry 1v4l
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Blood clotting
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PDB id
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1v4l
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Contents |
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* Residue conservation analysis
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Biochem J
378:399-407
(2004)
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PubMed id:
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Crystal structure of a platelet-agglutinating factor isolated from the venom of Taiwan habu (Trimeresurus mucrosquamatus).
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K.F.Huang,
T.P.Ko,
C.C.Hung,
J.Chu,
A.H.Wang,
S.H.Chiou.
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ABSTRACT
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Platelet glycoprotein Ib (GPIb)-binding proteins (GPIb-BPs) from snake venoms
are usually C-type lectins, which target specific sites of GPIbalpha and elicit
distinct effects on platelets. In the present paper, we report a tetrameric
platelet-agglutinating factor (molecular mass 121.1 kDa), termed mucrocetin,
purified from the venom of Taiwan habu (Trimeresurus mucrosquamatus ).
Mucrocetin is a GPIbalpha agonist with a binding site distinct from that of
flavocetin-A (a snake venom GPIbalpha antagonist) on GPIbalpha, in spite of the
high sequence identity (94.6%) between the two venom lectins. The crystal
structure of mucrocetin was solved and refined to 2.8 A (1 A=0.1 nm) resolution,
which shows an interesting crystal packing of six-layer cylinders of
doughnut-shaped molecules. The four alphabeta heterodimers are arranged in an
unusual square-shaped ring stabilized by four interdimer 'head-to-tail'
disulphide bridges. Detailed structural comparison between mucrocetin and
flavocetin-A suggests that their disparate platelet effects are probably
attributable to different charge distributions on the putative concave binding
surface. A unique positively charged patch on the binding surface of mucrocetin,
formed by Lys102, Lys108, Lys109 and Arg123 in the alpha-subunit coupled with
Lys22, Lys102, Lys116 and Arg117 in the beta-subunit, appears to be the primary
determinant of its platelet-agglutinating activity. Conceivably, this
interesting venom factor may provide a useful tool to study platelet
agglutination by binding to the GPIb-IX-V complex.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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T.Sajevic,
A.Leonardi,
and
I.Križaj
(2011).
Haemostatically active proteins in snake venoms.
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Toxicon,
57,
627-645.
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Z.Chen,
J.Wu,
Y.Zhang,
X.Yang,
G.Yu,
S.Zhu,
W.Lee,
Q.Lu,
and
Y.Zhang
(2011).
A novel platelet glycoprotein Ib-binding protein with human platelet aggregation-inhibiting activity from Trimeresurus jerdonii venom.
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Toxicon,
57,
672-679.
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A.Bazaa,
N.Marrakchi,
M.El Ayeb,
L.Sanz,
and
J.J.Calvete
(2005).
Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina.
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Proteomics,
5,
4223-4235.
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G.Xu,
Q.Huang,
M.Teng,
P.Liu,
Y.Dong,
and
L.Niu
(2005).
Crystallization and preliminary X-ray crystallographic analysis of agkicetin-C from Deinagkistrodon acutus venom.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
61,
75-78.
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G.Xu,
M.Teng,
L.Niu,
P.Liu,
Y.Dong,
Q.Liu,
Q.Huang,
and
Q.Hao
(2004).
Purification, characterization, crystallization and preliminary X-ray crystallographic analysis of two novel C-type lectin-like proteins: Aall-A and Aall-B from Deinagkistrodon acutus venom.
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Acta Crystallogr D Biol Crystallogr,
60,
2035-2037.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
