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PDBsum entry 1v2i
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure of the haemagglutinin-Neuraminidase from human parainfluenza virus type III.
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Authors
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M.C.Lawrence,
N.A.Borg,
V.A.Streltsov,
P.A.Pilling,
V.C.Epa,
J.N.Varghese,
J.L.Mckimm-Breschkin,
P.M.Colman.
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Ref.
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J Mol Biol, 2004,
335,
1343-1357.
[DOI no: ]
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PubMed id
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Abstract
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The three-dimensional structure of the haemagglutinin-neuraminidase (HN) from a
human parainfluenza virus is described at ca 2.0 A resolution, both in native
form and in complex with three substrate analogues. In support of earlier work
on the structure of the homologous protein from the avian pathogen Newcastle
disease virus (NDV), we observe a dimer of beta-propellers and find no evidence
for spatially separated sites performing the receptor-binding and neuraminidase
functions of the protein. As with the NDV HN, the active site of the HN of
parainfluenza viruses is structurally flexible, suggesting that it may be able
to switch between a receptor-binding state and a catalytic state. However, in
contrast to the NDV structures, we observe no ligand-induced structural changes
that extend beyond the active site and modify the dimer interface.
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Figure 3.
Figure 3. Stereo image of the unliganded PIV3 HN active
site in the hexagonal (green) and orthorhombic (yellow) crystal
forms. Only the "up" conformer of the side-chain of Y530 is
shown for the hexagonal crystal form. The two active-site water
molecules described in the text, w1 and w2, are shown, colored
red for in the hexagonal crystal form and black for in the
orthorhombic crystal form. Potential hydrogen bonds are shown as
black dotted lines. The Figure was produced using MOLSCRIPT[55.]
and Raster3D. [56.]
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Figure 4.
Figure 4. Stereo images of the active site of PIV3 HN
complexed with (a) b-sialic acid, (b) Neu5Ac2en and (c)
zanamivir. Two active-site water molecules are shown (w1 and w2,
see the text). Potential hydrogen bonds are shown as black
dotted lines. The Figure was produced using MOLSCRIPT[55.] and
Raster3D. [56.]
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The above figures are
reprinted
by permission from Elsevier:
J Mol Biol
(2004,
335,
1343-1357)
copyright 2004.
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