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UniProt functional annotation for O42807 | ||
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| UniProt code: O42807. |
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| Organism: | |
Aspergillus niger. |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati. |
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| Function: | |
Involved in degradation of plant cell walls. Hydrolyzes the feruloyl-arabinose ester bond in arabinoxylans, and the feruloyl- galactose ester bond in pectin. Binds to cellulose. {ECO:0000269|PubMed:11931668, ECO:0000269|PubMed:15081808, ECO:0000269|PubMed:17027758, ECO:0000269|PubMed:7805053, ECO:0000269|PubMed:9406381, ECO:0000269|PubMed:9649839, ECO:0000269|Ref.5}. |
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| Catalytic activity: | |
Reaction=Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide.; EC=3.1.1.73; |
| Activity regulation: | |
Inhibited by the specific serine esterase inhibitor diisopropylfluorophosphate. {ECO:0000269|PubMed:9649839}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.31 mM for methyl ferulate {ECO:0000269|PubMed:17027758}; pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:17027758}; Temperature dependence: Optimum temperature is 55-60 degrees Celsius. {ECO:0000269|PubMed:17027758}; |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:9406381, ECO:0000269|Ref.5}. |
| Induction: | |
By xylose and arabinose, probably via the xylanolytic transcriptional activator XlnR. By ferulic acid, vanillic acid and other aromatic residues with the following substituants on the aromatic ring: a methoxy group at C-3, a hydroxy group at C-4 and an unsubstituted C-5. Repressed by simple sugars, probably via the carbon catabolite repressor protein CreA. {ECO:0000269|PubMed:10584009, ECO:0000269|PubMed:11931668}. |
| Ptm: | |
Glycosylated. {ECO:0000305|PubMed:17027758}. |
| Mass spectrometry: | |
Mass=29738; Mass_error=50; Method=MALDI; Evidence={ECO:0000269|PubMed:9406381}; |
| Similarity: | |
Belongs to the AB hydrolase superfamily. FaeA family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.