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PDBsum entry 1uva
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Lipid transport
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PDB id
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1uva
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References listed in PDB file
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Key reference
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Title
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Lipid binding in rice nonspecific lipid transfer protein-1 complexes from oryza sativa.
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Authors
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H.C.Cheng,
P.T.Cheng,
P.Peng,
P.C.Lyu,
Y.J.Sun.
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Ref.
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Protein Sci, 2004,
13,
2304-2315.
[DOI no: ]
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PubMed id
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Abstract
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Nonspecific lipid transfer proteins (nsLTPs) facilitate the transfer of
phospholipids, glycolipids, fatty acids and steroids between membranes, with
wide-ranging binding affinities. Three crystal structures of rice nsLTP1 from
Oryza sativa, complexed with myristic (MYR), palmitic (PAL) or stearic acid
(STE) were determined. The overall structures of the rice nsLTP1 complexes
belong to the four-helix bundle folding with a long C-terminal loop. The
nsLTP1-MYR and the nsLTP1-STE complexes bind a single fatty acid while the
nsLTP1-PAL complex binds two molecules of fatty acids. The C-terminal loop
region is elastic in order to accommodate a diverse range of lipid molecules.
The lipid molecules interact with the nsLTP1-binding cavity mainly with
hydrophobic interactions. Significant conformational changes were observed in
the binding cavity and the C-terminal loop of the rice nsLTP1 upon lipid binding.
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Figure 4.
Figure 4. (A-D) The molecular surface of the rice nsTLP1
complexes (nsLTP1-MYR, nsLTP1-PAL1 & 2, and nsLTP1-STE A & B)
drawn by DS ViewerPro, colored according to the electrostatic
potential, ranging from blue to red (-10.0 to +10.0). Residues
77-82 were removed to show the hydrophobic cavity. Labeled
residues are those of rice nsLTP1 participating in hydrophobic
interactions between protein and fatty acids as shown in Table 1
Go- . (E-H) A
representation of the hydrophobic interactions between rice
nsLTP1 and fatty acids (MYR, PAL, STE A, and STE B) by LIGPLOT
(Wallace et al. 1995).
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Figure 5.
Figure 5. Hydrogen bond interactions between the head group
of fatty acid and the rice nsLTP1: (A) the myristic acid (MYR)
in the nsLTP1-MYR complex, (B) the first palmitic acid (PAL1) in
the nsLTP1-PAL complex, (C) the second palmitic acid (PAL2) in
the nsLTP1-PAL complex, and (D) the stearic acid (STE B) in the
nsLTP1-STE complex.
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The above figures are
reprinted
by permission from the Protein Society:
Protein Sci
(2004,
13,
2304-2315)
copyright 2004.
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