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PDBsum entry 1umr
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protein Protein-protein interface(s) links
Sugar binding protein PDB id
1umr

 

 

 

 

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Contents
Protein chains
135 a.a. *
125 a.a. *
Waters ×197
* Residue conservation analysis
PDB id:
1umr
Name: Sugar binding protein
Title: Crystal structure of the platelet activator convulxin, a disulfide linked a4b4 cyclic tetramer from the venom of crotalus durissus terrificus
Structure: Convulxin alpha. Chain: a, b. Synonym: cvx alpha. Convulxin beta. Chain: c, d. Synonym: cvx beta
Source: Crotalus durissus terrificus. South american rattlesnake. Organism_taxid: 8732. Tissue: venom gland. Tissue: venom gland
Biol. unit: Octamer (from PDB file)
Resolution:
2.40Å     R-factor:   0.190     R-free:   0.264
Authors: M.T.Murakami,S.P.Zela,L.M.Gava,S.Michelan-Duarte,A.C.O.Cintra, R.K.Arni
Key ref: M.T.Murakami et al. (2003). Crystal structure of the platelet activator convulxin, a disulfide-linked alpha4beta4 cyclic tetramer from the venom of Crotalus durissus terrificus. Biochem Biophys Res Commun, 310, 478-482. PubMed id: 14521935 DOI: 10.1016/j.bbrc.2003.09.032
Date:
28-Aug-03     Release date:   21-Nov-03    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
O93426  (SLA_CRODU) -  Snaclec convulxin subunit alpha from Crotalus durissus terrificus
Seq:
Struc: 		158 a.a.
135 a.a.
Protein chains
Pfam   ArchSchema ?
O93427  (SLB_CRODU) -  Snaclec convulxin subunit beta from Crotalus durissus terrificus
Seq:
Struc: 		148 a.a.
125 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1016/j.bbrc.2003.09.032 Biochem Biophys Res Commun 310:478-482 (2003)
PubMed id: 14521935  
 
 
Crystal structure of the platelet activator convulxin, a disulfide-linked alpha4beta4 cyclic tetramer from the venom of Crotalus durissus terrificus.
M.T.Murakami, S.P.Zela, L.M.Gava, S.Michelan-Duarte, A.C.Cintra, R.K.Arni.
 
  ABSTRACT  
 
Convulxin (CVX), a C-type lectin, isolated from the venom of the South American rattlesnake Crotalus durissus terrificus, causes cardiovascular and respiratory disturbances and is a potent platelet activator which binds to platelet glycoprotein GPVI. The structure of CVX has been solved at 2.4A resolution to a crystallographic residual of 18.6% (R(free)=26.4%). CVX is a disulfide linked heterodimer consisting of homologous alpha and beta chains. The heterodimers are additionally linked by disulfide bridges to form cyclic alpha(4)beta(4)heterotetramers. These domains exhibit significant homology to the carbohydrate-binding domains of C-type lectins, to the factor IX-binding protein (IX-bp), and to flavocetin-A (Fl-A) but sequence and structural differences are observed in both the domains in the putative Ca(2+)and carbohydrate binding regions.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21277886 T.Sajevic, A.Leonardi, and I.Križaj (2011).
Haemostatically active proteins in snake venoms.
  Toxicon, 57, 627-645.  
19495561 R.Doley, and R.M.Kini (2009).
Protein complexes in snake venom.
  Cell Mol Life Sci, 66, 2851-2871.  
18597489 E.Hooley, E.Papagrigoriou, A.Navdaev, A.V.Pandey, J.M.Clemetson, K.J.Clemetson, and J.Emsley (2008).
The crystal structure of the platelet activator aggretin reveals a novel (alphabeta)2 dimeric structure.
  Biochemistry, 47, 7831-7837.
PDB code: 3bx4
17764536 M.G.Tomlinson, S.D.Calaminus, O.Berlanga, J.M.Auger, T.Bori-Sanz, L.Meyaard, and S.P.Watson (2007).
Collagen promotes sustained glycoprotein VI signaling in platelets and cell lines.
  J Thromb Haemost, 5, 2274-2283.  
16689765 K.Kato, K.Furihata, Y.Cheli, G.Radis-Baptista, and T.J.Kunicki (2006).
Effect of multimer size and a natural dimorphism on the binding of convulxin to platelet glycoprotein (GP)VI.
  J Thromb Haemost, 4, 1107-1113.  
16206329 A.Bazaa, N.Marrakchi, M.El Ayeb, L.Sanz, and J.J.Calvete (2005).
Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina.
  Proteomics, 5, 4223-4235.  
  16508096 G.Xu, Q.Huang, M.Teng, P.Liu, Y.Dong, and L.Niu (2005).
Crystallization and preliminary X-ray crystallographic analysis of agkicetin-C from Deinagkistrodon acutus venom.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 75-78.  
15502319 G.Xu, M.Teng, L.Niu, P.Liu, Y.Dong, Q.Liu, Q.Huang, and Q.Hao (2004).
Purification, characterization, crystallization and preliminary X-ray crystallographic analysis of two novel C-type lectin-like proteins: Aall-A and Aall-B from Deinagkistrodon acutus venom.
  Acta Crystallogr D Biol Crystallogr, 60, 2035-2037.  
14684891 T.Batuwangala, M.Leduc, J.M.Gibbins, C.Bon, and E.Y.Jones (2004).
Structure of the snake-venom toxin convulxin.
  Acta Crystallogr D Biol Crystallogr, 60, 46-53.
PDB code: 1uos
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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