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PDBsum entry 1ukm
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structural characterization of ems16, An antagonist of collagen receptor (gpia/iia) from the venom of echis multisquamatus.
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Authors
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K.Horii,
D.Okuda,
T.Morita,
H.Mizuno.
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Ref.
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Biochemistry, 2003,
42,
12497-12502.
[DOI no: ]
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PubMed id
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Abstract
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Snake venoms contain a number of hemostatically active C-type lectin-like
proteins (CLPs), which affect the blood coagulation system, endothelial cells,
and platelets. CLPs have broad similarities in structure and possess distinct
biological functions. EMS16, a CLP from Echis multisquamatus venom, which is a
potent and selective inhibitor of the collagen receptor, glycoprotein Ia/IIa
(integrin alpha2beta1), has been used in the present study to examine
structure-function relationships in venom CLPs by X-ray crystallography. The
structure of EMS16, determined at a resolution of 1.9 A, revealed a heterodimer
involved with domain swapping of the central loop as observed in the structures
of other CLPs. A part of the glycan was observed and identified as
N-acetyl-D-glucosamine (GlcNAc) in the electron density map at Asn21 of subunit
B, an expected glycosylation site. EMS16 had a unique, positively charged
electrostatic potential patch on the concave surface that may qualify as a site
for interaction with the I-domain of the glycoprotein Ia/IIa.
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Secondary reference #1
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Title
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Characterization and preliminary crystallographic studies of ems16, An antagonist of collagen receptor (gpia/iia) from the venom of echis multisquamatus.
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Authors
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D.Okuda,
K.Horii,
H.Mizuno,
T.Morita.
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Ref.
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J Biochem (tokyo), 2003,
134,
19-23.
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PubMed id
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Secondary reference #2
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Title
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Isolation and characterization of ems16, A c-Lectin type protein from echis multisquamatus venom, A potent and selective inhibitor of the alpha2beta1 integrin.
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Authors
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C.Marcinkiewicz,
R.R.Lobb,
M.M.Marcinkiewicz,
J.L.Daniel,
J.B.Smith,
C.Dangelmaier,
P.H.Weinreb,
D.A.Beacham,
S.Niewiarowski.
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Ref.
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Biochemistry, 2000,
39,
9859-9867.
[DOI no: ]
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PubMed id
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