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PDBsum entry 1ujc
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the protein histidine phosphatase sixa in the multistep his-Asp phosphorelay.
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Authors
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K.Hamada,
M.Kato,
T.Shimizu,
K.Ihara,
T.Mizuno,
T.Hakoshima.
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Ref.
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Genes Cells, 2005,
10,
1.
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PubMed id
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Abstract
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The multiple histidine-aspartate phosphorelay system plays a crucial role in
cellular adaptation to environments in microorganisms and plants. Like
kinase-phosphatase systems in higher eukaryotes, the multiple steps provide
additional regulatory checkpoints with phosphatases. The Escherichia coli
phosphatase SixA exhibits protein phosphatase activity against the
histidine-containing phosphotransfer (HPt) domain located in the C-terminus of
the histidine kinase ArcB engaged in anaerobic responses. We have determined the
crystal structures of the free and tungstate-bound forms of SixA at 2.06 A and
1.90 A resolution, respectively. The results provide the first three-dimensional
view of a bacterial protein histidine phosphatase, revealing a compact
alpha/beta architecture related to a family of phosphatases containing the
arginine-histidine-glycine (RHG) motif at their active sites. Compared with
these RHG phosphatases, SixA lacks an extra alpha-helical subdomain as a lid
over the active site, thereby forming a relatively shallow groove important for
the accommodation of the HPt domain of ArcB. The tungstate ion, which mimics the
substrate phosphate group, is located at the centre of the active site where the
active residue, His8, points to the tungsten atom in the mode of in-line
nucleophilic attack.
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Secondary reference #1
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Title
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Crystallographic characterization of a novel protein sixa which exhibits phospho-Histidine phosphatase activity in the multistep his-Asp phosphorelay.
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Authors
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K.Hamada,
M.Kato,
T.Mizuno,
T.Hakoshima.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1999,
55,
269-271.
[DOI no: ]
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PubMed id
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Figure 1.
Figure 1 Crystal of SixA. The scale bar is 0.3 mm long.
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The above figure is
reproduced from the cited reference
with permission from the IUCr
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