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UniProt functional annotation for P09147 | ||
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| UniProt code: P09147. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. It is only active on UDP-galactose and UDP-glucose. {ECO:0000269|PubMed:14235524}. |
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| Catalytic activity: | |
Reaction=UDP-alpha-D-glucose = UDP-alpha-D-galactose; Xref=Rhea:RHEA:22168, ChEBI:CHEBI:58885, ChEBI:CHEBI:66914; EC=5.1.3.2; Evidence={ECO:0000269|PubMed:14235524}; |
| Cofactor: | |
Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000269|PubMed:12019271, ECO:0000269|PubMed:8241178, ECO:0000269|PubMed:8611497, ECO:0000269|PubMed:8611559, ECO:0000269|PubMed:8652544, ECO:0000269|PubMed:8931134, ECO:0000269|PubMed:9174344, ECO:0000269|PubMed:9708982}; |
| Activity regulation: | |
Inhibited by UDP-phenol and NaBH3CN. {ECO:0000269|PubMed:1579570, ECO:0000269|PubMed:9271498}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=18 uM for UDP-Gal (at pH 8.5) {ECO:0000269|PubMed:14235524, ECO:0000269|PubMed:8241178, ECO:0000269|PubMed:9271498}; KM=160 uM for UDP-Gal (at pH 8.5 and 27 degrees Celsius) {ECO:0000269|PubMed:14235524, ECO:0000269|PubMed:8241178, ECO:0000269|PubMed:9271498}; KM=225 uM for UDP-Glc (at pH 8.5 and 27 degrees Celsius) {ECO:0000269|PubMed:14235524, ECO:0000269|PubMed:8241178, ECO:0000269|PubMed:9271498}; Note=Kcat is 760 sec(-1) for UDP-Glc (at pH 8.5 and 27 degrees Celsius) and 24 sec(-1) for UDP-Gal (at pH 8.5).; |
| Pathway: | |
Carbohydrate metabolism; galactose metabolism. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:12019271, ECO:0000269|PubMed:1579570, ECO:0000269|PubMed:4305667, ECO:0000269|PubMed:8611497, ECO:0000269|PubMed:8611559, ECO:0000269|PubMed:8652544, ECO:0000269|PubMed:8931134, ECO:0000269|PubMed:9174344, ECO:0000269|PubMed:9271498, ECO:0000269|PubMed:9271499, ECO:0000269|PubMed:9708982}. |
| Induction: | |
By D-galactose and D-fucose. {ECO:0000269|PubMed:14235524}. |
| Similarity: | |
Belongs to the NAD(P)-dependent epimerase/dehydratase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.