PDBsum entry 1ubc

Recombination

PDB id

1ubc

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Contents

			Protein chain

					303 a.a. *

			Waters ×25

* Residue conservation analysis

PDB id:

1ubc

Links

Name:

Recombination

Title:

Structure of reca protein

Structure:

Reca. Chain: a. Synonym: recombinase a. Engineered: yes

Source:

Mycobacterium smegmatis. Organism_taxid: 1772. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.80Å

R-factor:

0.242

R-free:

0.308

Authors:

S.Datta,R.Krishna,N.Ganesh,N.R.Chandra,K.Muniyappa,M.Vijayan

Key ref:

S.Datta et al. (2003). Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexes. J Bacteriol, 185, 4280-4284. PubMed id: 12837805

Date:

04-Apr-03

Release date:

22-Jul-03

PROCHECK

	Headers

	References

Protein chain

Q59560 (RECA_MYCS2) - Protein RecA from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

Seq: Struc:					349 a.a. 303 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

	J Bacteriol 185:4280-4284 (2003)
PubMed id: 12837805

Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexes.
S.Datta, R.Krishna, N.Ganesh, N.R.Chandra, K.Muniyappa, M.Vijayan.

ABSTRACT

The crystal structures of Mycobacterium smegmatis RecA (RecA(Ms)) and its complexes with ADP, ATPgammaS, and dATP show that RecA(Ms) has an expanded binding site like that in Mycobacterium tuberculosis RecA, although there are small differences between the proteins in their modes of nucleotide binding. Nucleotide binding is invariably accompanied by the movement of Gln 196, which appears to provide the trigger for transmitting the effect of nucleotide binding to the DNA-binding loops. These observations provide a framework for exploring the known properties of the RecA proteins.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21458462

V.E.Galkin, R.L.Britt, L.B.Bane, X.Yu, M.M.Cox, and E.H.Egelman (2011).
Two modes of binding of DinI to RecA filament provide a new insight into the regulation of SOS response by DinI protein.

J Mol Biol, 408, 815-824.

20615954

D.F.Warner, D.E.Ndwandwe, G.L.Abrahams, B.D.Kana, E.E.Machowski, C.Venclovas, and V.Mizrahi (2010).
Essential roles for imuA'- and imuB-encoded accessory factors in DnaE2-dependent mutagenesis in Mycobacterium tuberculosis.

Proc Natl Acad Sci U S A, 107, 13093-13098.

19465774

Y.Li, Y.He, and Y.Luo (2009).
Conservation of a conformational switch in RadA recombinase from Methanococcus maripaludis.

Acta Crystallogr D Biol Crystallogr, 65, 602-610.

PDB codes:

3etl 3ew9 3ewa

18579587

A.Kumar, W.S.Joo, G.Meinke, S.Moine, E.N.Naumova, and P.A.Bullock (2008).
Evidence for a structural relationship between BRCT domains and the helicase domains of the replication initiators encoded by the Polyomaviridae and Papillomaviridae families of DNA tumor viruses.

J Virol, 82, 8849-8862.

19020353

J.R.Prabu, G.P.Manjunath, N.R.Chandra, K.Muniyappa, and M.Vijayan (2008).
Functionally important movements in RecA molecules and filaments: studies involving mutation and environmental changes.

Acta Crystallogr D Biol Crystallogr, 64, 1146-1157.

PDB codes:

2zr0 2zr7 2zr9 2zra 2zrb 2zrc 2zrd 2zre 2zrf 2zrg 2zrh 2zri 2zrj 2zrk 2zrl 2zrm 2zrn 2zro 2zrp

18453691

P.S.Kaushal, R.K.Talawar, P.D.Krishna, U.Varshney, and M.Vijayan (2008).
Unique features of the structure and interactions of mycobacterial uracil-DNA glycosylase: structure of a complex of the Mycobacterium tuberculosis enzyme in comparison with those from other sources.

Acta Crystallogr D Biol Crystallogr, 64, 551-560.

PDB code:

2zhx

17228330

M.M.Cox (2007).
Motoring along with the bacterial RecA protein.

Nat Rev Mol Cell Biol, 8, 127-138.

16880543

J.R.Prabu, S.Thamotharan, J.S.Khanduja, E.Z.Alipio, C.Y.Kim, G.S.Waldo, T.C.Terwilliger, B.Segelke, T.Lekin, D.Toppani, L.W.Hung, M.Yu, E.Bursey, K.Muniyappa, N.R.Chandra, and M.Vijayan (2006).
Structure of Mycobacterium tuberculosis RuvA, a protein involved in recombination.

Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 731-734.

PDB code:

2h5x

16909421

M.Petukhov, D.Lebedev, V.Shalguev, A.Islamov, A.Kuklin, V.Lanzov, and V.Isaev-Ivanov (2006).
Conformational flexibility of RecA protein filament: transitions between compressed and stretched states.

Proteins, 65, 296-304.

16648362

R.Krishna, G.P.Manjunath, P.Kumar, A.Surolia, N.R.Chandra, K.Muniyappa, and M.Vijayan (2006).
Crystallographic identification of an ordered C-terminal domain and a second nucleotide-binding site in RecA: new insights into allostery.

Nucleic Acids Res, 34, 2186-2195.

PDB code:

2g88

16699190

S.Das, P.Kumar, V.Bhor, A.Surolia, and M.Vijayan (2006).
Invariance and variability in bacterial PanK: a study based on the crystal structure of Mycobacterium tuberculosis PanK.

Acta Crystallogr D Biol Crystallogr, 62, 628-638.

PDB codes:

2ges 2get 2geu 2gev

16765891

V.E.Galkin, Y.Wu, X.P.Zhang, X.Qian, Y.He, X.Yu, W.D.Heyer, Y.Luo, and E.H.Egelman (2006).
The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity.

Structure, 14, 983-992.

PDB code:

2gdj

15755748

A.Ariza, D.J.Richard, M.F.White, and C.S.Bond (2005).
Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA.

Nucleic Acids Res, 33, 1465-1473.

PDB code:

2bke

16194225

C.E.Bell (2005).
Structure and mechanism of Escherichia coli RecA ATPase.

Mol Microbiol, 58, 358-366.

16508093

S.Das, P.Kumar, V.Bhor, A.Surolia, and M.Vijayan (2005).
Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis.

Acta Crystallogr Sect F Struct Biol Cryst Commun, 61, 65-67.

15956102

T.Akiba, N.Ishii, N.Rashid, M.Morikawa, T.Imanaka, and K.Harata (2005).
Structure of RadB recombinase from a hyperthermophilic archaeon, Thermococcus kodakaraensis KOD1: an implication for the formation of a near-7-fold helical assembly.

Nucleic Acids Res, 33, 3412-3423.

PDB codes:

2cvf 2cvh

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.