UniProt functional annotation for P25779



	UniProt functional annotation for P25779

UniProt code: P25779.

Organism: Trypanosoma cruzi.

Taxonomy: Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.

Function: Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group.

Function: The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.

Catalytic activity: Reaction=Broad endopeptidase specificity similar to that of cathepsin L.; EC=3.4.22.51;

Activity regulation: Strongly inhibited by E-64 (L-trans- epoxysuccinylleucylamido(4-guanidino)butane), Leupeptin, and N-alpha-p- tosyl-L-lysine chloromethyl ketone.

Developmental stage: Present in all developmental stages.

Miscellaneous: Purified cruzipain is able to degrade itself, yielding a complex mixture of small peptides, and a major 25 kDa fragment.

Similarity: Belongs to the peptidase C1 family. {ECO:0000255|PROSITE- ProRule:PRU10088, ECO:0000255|PROSITE-ProRule:PRU10089, ECO:0000255|PROSITE-ProRule:PRU10090}.

Annotations taken from UniProtKB at the EBI.


Organism:		Trypanosoma cruzi.
Taxonomy:		Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.



Function:		Hydrolyzes chromogenic peptides at the carboxyl Arg or Lys; requires at least one more amino acid, preferably Arg, Phe, Val or Leu, between the terminal Arg or Lys and the amino-blocking group.



Function:		The cysteine protease may play an important role in the development and differentiation of the parasites at several stages of their life cycle.


Catalytic activity:		Reaction=Broad endopeptidase specificity similar to that of cathepsin L.; EC=3.4.22.51;
Activity regulation:		Strongly inhibited by E-64 (L-trans- epoxysuccinylleucylamido(4-guanidino)butane), Leupeptin, and N-alpha-p- tosyl-L-lysine chloromethyl ketone.
Developmental stage:		Present in all developmental stages.
Miscellaneous:		Purified cruzipain is able to degrade itself, yielding a complex mixture of small peptides, and a major 25 kDa fragment.
Similarity:		Belongs to the peptidase C1 family. {ECO:0000255\|PROSITE- ProRule:PRU10088, ECO:0000255\|PROSITE-ProRule:PRU10089, ECO:0000255\|PROSITE-ProRule:PRU10090}.