UniProt functional annotation for P0A7G6



	UniProt functional annotation for P0A7G6

UniProt code: P0A7G6.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single- stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). {ECO:0000269|PubMed:22412352, ECO:0000269|PubMed:26845522, ECO:0000269|PubMed:7608206, ECO:0000269|PubMed:9230304}.

Activity regulation: The rate of DNA-strand exchange is stimulated by RadA. {ECO:0000269|PubMed:26845522}.

Subunit: Polymerizes non-specifically on ssDNA to form filaments; filament formation requires ATP or ATP-gamma-S. Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair. Interacts with the C-terminus of RecB, facilitating loading of RecA onto ssDNA at chi sites. Interaction is decreased by ATP. {ECO:0000269|PubMed:16483938, ECO:0000269|PubMed:1731246, ECO:0000269|PubMed:1731253}.

Subcellular location: Cytoplasm.

Induction: Induced by DNA damage, repressed by LexA (PubMed:10760155). Induced in response to low temperature (PubMed:8898389). Sensitive to temperature through changes in the linking number of the DNA. Induced by cold shock (42 to 15 degrees Celsius) (at protein level) (PubMed:8898389). 5.1-fold induced by hydroxyurea treatment (at protein level) (PubMed:20005847). mRNA levels are repressed in a mazE-mazF- mediated manner (PubMed:22412352). {ECO:0000269|PubMed:10760155, ECO:0000269|PubMed:20005847, ECO:0000269|PubMed:22412352, ECO:0000269|PubMed:8898389}.

Disruption phenotype: Triple mazE-mazF-recA mutant cells no longer undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization. {ECO:0000269|PubMed:22412352}.

Similarity: Belongs to the RecA family. {ECO:0000255|HAMAP- Rule:MF_00268}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Required for homologous recombination and the bypass of mutagenic DNA lesions by the SOS response. Catalyzes ATP-driven homologous pairing and strand exchange of DNA molecules necessary for DNA recombinational repair. Catalyzes the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single- stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. The SOS response controls an apoptotic-like death (ALD) induced (in the absence of the mazE-mazF toxin-antitoxin module) in response to DNA damaging agents that is mediated by RecA and LexA (PubMed:22412352). {ECO:0000269\|PubMed:22412352, ECO:0000269\|PubMed:26845522, ECO:0000269\|PubMed:7608206, ECO:0000269\|PubMed:9230304}.


Activity regulation:		The rate of DNA-strand exchange is stimulated by RadA. {ECO:0000269\|PubMed:26845522}.
Subunit:		Polymerizes non-specifically on ssDNA to form filaments; filament formation requires ATP or ATP-gamma-S. Interacts with and activates LexA leading to autocatalytic cleavage of LexA, which derepresses the SOS regulon and activates DNA repair. Interacts with the C-terminus of RecB, facilitating loading of RecA onto ssDNA at chi sites. Interaction is decreased by ATP. {ECO:0000269\|PubMed:16483938, ECO:0000269\|PubMed:1731246, ECO:0000269\|PubMed:1731253}.
Subcellular location:		Cytoplasm.
Induction:		Induced by DNA damage, repressed by LexA (PubMed:10760155). Induced in response to low temperature (PubMed:8898389). Sensitive to temperature through changes in the linking number of the DNA. Induced by cold shock (42 to 15 degrees Celsius) (at protein level) (PubMed:8898389). 5.1-fold induced by hydroxyurea treatment (at protein level) (PubMed:20005847). mRNA levels are repressed in a mazE-mazF- mediated manner (PubMed:22412352). {ECO:0000269\|PubMed:10760155, ECO:0000269\|PubMed:20005847, ECO:0000269\|PubMed:22412352, ECO:0000269\|PubMed:8898389}.
Disruption phenotype:		Triple mazE-mazF-recA mutant cells no longer undergo an apoptotic-like death upon DNA damage characterized by membrane depolarization. {ECO:0000269\|PubMed:22412352}.
Similarity:		Belongs to the RecA family. {ECO:0000255\|HAMAP- Rule:MF_00268}.