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UniProt functional annotation for P00952 | ||
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| UniProt code: P00952. |
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| Organism: | |
Geobacillus stearothermophilus (Bacillus stearothermophilus). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. |
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| Function: | |
Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two- step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). |
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| Catalytic activity: | |
Reaction=ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L- tyrosyl-tRNA(Tyr); Xref=Rhea:RHEA:10220, Rhea:RHEA-COMP:9706, Rhea:RHEA-COMP:9707, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58315, ChEBI:CHEBI:78442, ChEBI:CHEBI:78536, ChEBI:CHEBI:456215; EC=6.1.1.1; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.35 mM for ATP {ECO:0000269|PubMed:6315404}; KM=1.8 uM for tyrosine {ECO:0000269|PubMed:6315404}; |
| Subunit: | |
Homodimer. |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.