PDBsum entry 1ttx

Go to PDB code: 
protein metals links
Metal binding protein PDB id
Jmol PyMol
Protein chain
109 a.a. *
_CA ×2
* Residue conservation analysis
PDB id:
Name: Metal binding protein
Title: Solution structure of human beta parvalbumin (oncomodulin) r with a paramagnetism based strategy
Structure: Oncomodulin. Chain: a. Synonym: beta parvalbumin, om. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: homo sapiens. Expressed in: escherichia coli. Expression_system_taxid: 562.
NMR struc: 20 models
Authors: E.Babini,I.Bertini,F.Capozzi,C.Del Bianco,D.Hollender,T.Kiss C.Luchinat,A.Quattrone,Structural Proteomics In Europe (Spi
Key ref:
E.Babini et al. (2004). Solution structure of human beta-parvalbumin and structural comparison with its paralog alpha-parvalbumin and with their rat orthologs. Biochemistry, 43, 16076-16085. PubMed id: 15610002 DOI: 10.1021/bi048388o
23-Jun-04     Release date:   18-Jan-05    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P0CE72  (ONCO_HUMAN) -  Oncomodulin-1
109 a.a.
109 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     metal ion binding     2 terms  


DOI no: 10.1021/bi048388o Biochemistry 43:16076-16085 (2004)
PubMed id: 15610002  
Solution structure of human beta-parvalbumin and structural comparison with its paralog alpha-parvalbumin and with their rat orthologs.
E.Babini, I.Bertini, F.Capozzi, C.Del Bianco, D.Hollender, T.Kiss, C.Luchinat, A.Quattrone.
The aim of this research was to determine the structure of human beta-parvalbumin (109 amino acids) and to compare it with its paralog and ortholog proteins. The structure was determined in solution using multinuclear and multidimensional NMR methods and refined using substitution of the EF-hand Ca(2+) ion with a paramagnetic lanthanide. The resulting family of structures had a backbone rmsd of 0.50 A. Comparison with rat oncomodulin (X-ray, 1.3 A resolution) as well as with human (NMR, backbone rmsd of 0.49 A) and rat (X-ray, 2.0 A resolution) parvalbumins reveals small but reliable local differences, often but not always related to amino acid variability. The analysis of these structures has led us to propose an explanation for the different affinity for Ca(2+) between alpha- and beta-parvalbumins and between parvalbumins and calmodulins.

Literature references that cite this PDB file's key reference

  PubMed id Reference
17558470 J.A.Vila, M.E.Villegas, H.A.Baldoni, and H.A.Scheraga (2007).
Predicting 13Calpha chemical shifts for validation of protein structures.
  J Biomol NMR, 38, 221-235.  
17766386 M.T.Henzl, and J.J.Tanner (2007).
Solution structure of Ca2+-free rat beta-parvalbumin (oncomodulin).
  Protein Sci, 16, 1914-1926.
PDB code: 2nln
17094115 S.J.Lee, C.C.Ju, S.L.Chu, M.S.Chien, T.H.Chan, and W.L.Liao (2007).
Molecular cloning, expression and phylogenetic analyses of parvalbumin in tilapia, Oreochromis mossambicus.
  J Exp Zool Part A Ecol Genet Physiol, 307, 51-61.  
17001092 E.Ab, A.R.Atkinson, L.Banci, I.Bertini, S.Ciofi-Baffoni, K.Brunner, T.Diercks, V.Dötsch, F.Engelke, G.E.Folkers, C.Griesinger, W.Gronwald, U.Günther, M.Habeck, Jong, H.R.Kalbitzer, B.Kieffer, B.R.Leeflang, S.Loss, C.Luchinat, T.Marquardsen, D.Moskau, K.P.Neidig, M.Nilges, M.Piccioli, R.Pierattelli, W.Rieping, T.Schippmann, H.Schwalbe, G.Travé, J.Trenner, J.Wöhnert, M.Zweckstetter, and R.Kaptein (2006).
NMR in the SPINE Structural Proteomics project.
  Acta Crystallogr D Biol Crystallogr, 62, 1150-1161.  
16765896 E.Babini, I.Bertini, F.Capozzi, E.Chirivino, and C.Luchinat (2006).
A structural and dynamic characterization of the EF-hand protein CLSP.
  Structure, 14, 1029-1038.
PDB code: 2b1u
16957918 F.Capozzi, F.Casadei, and C.Luchinat (2006).
EF-hand protein dynamics and evolution of calcium signal transduction: an NMR view.
  J Biol Inorg Chem, 11, 949-962.  
16258835 E.Babini, I.C.Felli, M.Lelli, C.Luchinat, and R.Pierattelli (2005).
Backbone and side-chains 1H, 13C and 15N NMR assignment of human beta-parvalbumin.
  J Biomol NMR, 33, 137.  
16094696 I.Bertini, C.Luchinat, G.Parigi, and R.Pierattelli (2005).
NMR spectroscopy of paramagnetic metalloproteins.
  Chembiochem, 6, 1536-1549.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.