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PDBsum entry 1tbs
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Serine protease
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PDB id
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1tbs
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References listed in PDB file
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Key reference
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Title
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Structure determination and refinement of benzamidine-Inhibited trypsin from the north atlantic salmon (salmo salar) at 1.82 a resolution.
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Authors
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A.Smalås,
A.Hordvik.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1993,
49,
318-330.
[DOI no: ]
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PubMed id
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Abstract
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The structure of the serine protease trypsin from the North Atlantic salmon
(Salmo salar) has been solved by molecular replacement and refined by restrained
least-squares methods to a conventional R factor of 16.4% using diffractometer
data in the 6.0-1.82 A resolution range (14 443 reflections greater than
3sigma). The model comprises 1793 protein atoms and 180 solvent molecules which
were given unit occupancies, and the average temperature factors for protein
atoms and solvent oxygen atoms are 15.2 and 36.8 A(2), respectively. The
estimated error in atomic positions is about 0.2 A. The structure of salmon
trypsin was solved and refined with only a small part of the amino-acid sequence
known. However, a gene sequence of salmon trypsin has later become available.
Some discrepancies between this sequence and the sequence obtained from the
present X-ray crystal study indicate that the mentioned sequences may correspond
to isoenzymes. The structure of salmon trypsin is similar to other trypsins of
known structure.
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Figure 3.
Fig. 3. Unclear electron density in the region 146149.
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Figure 6.
Fig. 6. The benzamidine molecule in the activesite cleft.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(1993,
49,
318-330)
copyright 1993.
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Secondary reference #1
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Title
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Crystallization and preliminary X-Ray crystallographic studies of benzamidine-Inhibited trypsin from the north atlantic salmon (salmo salar).
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Authors
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A.O.Smalås,
A.Hordvik,
L.K.Hansen,
E.Hough,
K.Jynge.
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Ref.
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J Mol Biol, 1990,
214,
355-358.
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PubMed id
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Secondary reference #2
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Title
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The geometry of the reactive site and of the peptide groups in trypsin, Trypsinogen and its complexes with inhibitors
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Authors
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M.Marquart,
J.Walter,
J.Deisenhofer,
W.Bode,
R.Huber.
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Ref.
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acta crystallogr ,sect b, 1983,
39,
480.
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