UniProt functional annotation for O34530



	UniProt functional annotation for O34530

UniProt code: O34530.

Organism: Bacillus subtilis (strain 168).

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.

Function: One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP (PubMed:16485133). GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation (PubMed:22544754). Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo (PubMed:15828870). Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes (PubMed:15828870). Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis (PubMed:18344364). {ECO:0000269|PubMed:15828870, ECO:0000269|PubMed:16485133, ECO:0000269|PubMed:18344364, ECO:0000269|PubMed:22544754}.

Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|HAMAP-Rule:MF_01820, ECO:0000269|PubMed:15223319}; Note=Binds 1 zinc ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01820, ECO:0000269|PubMed:15223319};

Biophysicochemical properties: Kinetic parameters: KM=30.5 uM for GTP {ECO:0000269|PubMed:16485133}; Note=kcat for GTP is 13.6 hour(-1), in the absence of ribosomes. {ECO:0000269|PubMed:16485133};

Subunit: Monomer, but able to form dimers (PubMed:16485133). Associates with 30S ribosomal subunit; a phospho-mimetic mutation increases association (PubMed:22544754). Probably binds 16S rRNA. {ECO:0000269|PubMed:16485133, ECO:0000269|PubMed:22544754, ECO:0000305|PubMed:15223319}.

Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01820}.

Ptm: In vitro phosphorylated mostly on Thr (with lower signal on Ser) by PrkC in the presence of poly-L-Lys or myelin basic protein, dephosphorylated by PrpC (PubMed:19246764). Most in vitro phosphorylation occurs on Thr-166, in vivo phosphorylation has not been detected, but it might vary during the cell cycle (PubMed:22544754). {ECO:0000269|PubMed:19246764, ECO:0000269|PubMed:22544754}.

Disruption phenotype: Has been described as essential (PubMed:9743119, PubMed:16485133, PubMed:18344364), but also as non-essential (PubMed:15828870). Cells have a slow growth phenotype (PubMed:15828870, PubMed:16485133, PubMed:18344364, PubMed:22544754). Disrupted strain grows as chains of filaments, a cell curvature phenotype is also present, resulting in long wavy cells or short curved rods (PubMed:15828870, PubMed:22544754). Depleted cells form aberrant, swollen cells (PubMed:16485133, PubMed:18344364). Depleted cells DNA staining shows fragmented and/or disturbed nucleoid segregation; effects are seen most in minimal E-medium (PubMed:16485133). Depleted cells have an irregular deposition of cell wall and 15% have abnormal septal cleavage planes (PubMed:18344364). {ECO:0000269|PubMed:15828870, ECO:0000269|PubMed:16485133, ECO:0000269|PubMed:18344364, ECO:0000269|PubMed:9743119}.

Similarity: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily. {ECO:0000255|HAMAP-Rule:MF_01820, ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus subtilis (strain 168).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.



Function:		One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP (PubMed:16485133). GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation (PubMed:22544754). Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo (PubMed:15828870). Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes (PubMed:15828870). Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis (PubMed:18344364). {ECO:0000269\|PubMed:15828870, ECO:0000269\|PubMed:16485133, ECO:0000269\|PubMed:18344364, ECO:0000269\|PubMed:22544754}.


Cofactor:		Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255\|HAMAP-Rule:MF_01820, ECO:0000269\|PubMed:15223319}; Note=Binds 1 zinc ion per subunit. {ECO:0000255\|HAMAP-Rule:MF_01820, ECO:0000269\|PubMed:15223319};
Biophysicochemical properties:		Kinetic parameters: KM=30.5 uM for GTP {ECO:0000269\|PubMed:16485133}; Note=kcat for GTP is 13.6 hour(-1), in the absence of ribosomes. {ECO:0000269\|PubMed:16485133};
Subunit:		Monomer, but able to form dimers (PubMed:16485133). Associates with 30S ribosomal subunit; a phospho-mimetic mutation increases association (PubMed:22544754). Probably binds 16S rRNA. {ECO:0000269\|PubMed:16485133, ECO:0000269\|PubMed:22544754, ECO:0000305\|PubMed:15223319}.
Subcellular location:		Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_01820}.
Ptm:		In vitro phosphorylated mostly on Thr (with lower signal on Ser) by PrkC in the presence of poly-L-Lys or myelin basic protein, dephosphorylated by PrpC (PubMed:19246764). Most in vitro phosphorylation occurs on Thr-166, in vivo phosphorylation has not been detected, but it might vary during the cell cycle (PubMed:22544754). {ECO:0000269\|PubMed:19246764, ECO:0000269\|PubMed:22544754}.
Disruption phenotype:		Has been described as essential (PubMed:9743119, PubMed:16485133, PubMed:18344364), but also as non-essential (PubMed:15828870). Cells have a slow growth phenotype (PubMed:15828870, PubMed:16485133, PubMed:18344364, PubMed:22544754). Disrupted strain grows as chains of filaments, a cell curvature phenotype is also present, resulting in long wavy cells or short curved rods (PubMed:15828870, PubMed:22544754). Depleted cells form aberrant, swollen cells (PubMed:16485133, PubMed:18344364). Depleted cells DNA staining shows fragmented and/or disturbed nucleoid segregation; effects are seen most in minimal E-medium (PubMed:16485133). Depleted cells have an irregular deposition of cell wall and 15% have abnormal septal cleavage planes (PubMed:18344364). {ECO:0000269\|PubMed:15828870, ECO:0000269\|PubMed:16485133, ECO:0000269\|PubMed:18344364, ECO:0000269\|PubMed:9743119}.
Similarity:		Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA subfamily. {ECO:0000255\|HAMAP-Rule:MF_01820, ECO:0000305}.