UniProt functional annotation for P56981



	UniProt functional annotation for P56981

UniProt code: P56981.

Organism: Bacillus caldotenax.

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; Geobacillus thermoleovorans group.

Function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity). {ECO:0000250}.

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;

Subunit: Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.

Subcellular location: Cytoplasm {ECO:0000250}.

Domain: The beta-hairpin motif is involved in DNA binding.

Similarity: Belongs to the UvrB family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus caldotenax.
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; Geobacillus thermoleovorans group.



Function:		The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage (By similarity). {ECO:0000250}.


Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
Subunit:		Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.
Subcellular location:		Cytoplasm {ECO:0000250}.
Domain:		The beta-hairpin motif is involved in DNA binding.
Similarity:		Belongs to the UvrB family. {ECO:0000305}.