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PDBsum entry 1sxl
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RNA binding protein
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PDB id
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1sxl
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References listed in PDB file
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Key reference
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Title
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Resonance assignments and solution structure of the second RNA-Binding domain of sex-Lethal determined by multidimensional heteronuclear magnetic resonance.
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Authors
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A.L.Lee,
R.Kanaar,
D.C.Rio,
D.E.Wemmer.
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Ref.
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Biochemistry, 1994,
33,
13775-13786.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
96%.
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Abstract
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The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual
differentiation and dosage compensation in Drosophila. This regulatory activity
is a consequence of the ability of Sxl to bind uridine-rich RNA tracts involved
in pre-mRNA splicing. Sxl contains two RNP consensus-type RNA-binding domains
(RBDs). A structural study of a portion of Sxl (amino acids 199-294) containing
the second RNA-binding domain (RBD-2) using multidimensional heteronuclear NMR
is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have
been obtained from 15N- and 13C/15N-uniformly labeled protein. These assignments
were used to analyze 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY
spectra which produced 494 total and 169 long-range NOE-derived distance
restraints. Along with 41 backbone dihedral restraints, these distance
restraints were employed to generate an intermediate-resolution family of
calculated structures, which exhibits the beta alpha beta-beta alpha beta
tertiary fold found in other RBD-containing proteins. The RMSD to the average
structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the
RMSD for backbone atoms involved in secondary structure is 0.76 +/- 0.14 A. A
capping box [Harper, E.T., & Rose, G.D. (1993) Biochemistry 32, 7605-7609]
was identified at the N-terminus of the first helix and has been characterized
by short- and medium-range NOEs. Finally, significant structural similarities
and differences between Sxl RBD-2 and other RBD-containing proteins are
discussed.
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Secondary reference #1
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Title
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Determination of the secondary structure and folding topology of an RNA binding domain of mammalian hnrnp a1 protein using three-Dimensional heteronuclear magnetic resonance spectroscopy.
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Authors
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D.S.Garrett,
P.J.Lodi,
Y.Shamoo,
K.R.Williams,
G.M.Clore,
A.M.Gronenborn.
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Ref.
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Biochemistry, 1994,
33,
2852-2858.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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1h, 13c, And 15n nmr assignments and global folding pattern of the RNA-Binding domain of the human hnrnp c proteins.
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Authors
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M.Wittekind,
M.Görlach,
M.Friedrichs,
G.Dreyfuss,
L.Mueller.
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Ref.
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Biochemistry, 1992,
31,
6254-6265.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystal structure of the RNA-Binding domain of the u1 small nuclear ribonucleoprotein a.
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Authors
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K.Nagai,
C.Oubridge,
T.H.Jessen,
J.Li,
P.R.Evans.
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Ref.
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Nature, 1990,
348,
515-520.
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PubMed id
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