PDBsum entry 1swi

Leucine zipper

PDB id: 1swi

Contents

Protein chains

31 a.a. *

30 a.a. *

Ligands

BNZ

Waters ×29

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: An engineered allosteric switch in leucine-Zipper oligomerization.
• Authors: L.Gonzalez, J.J.Plecs, T.Alber.
• Ref.: Nat Struct Biol, 1996, 3, 510-515.
• PubMed id: 8646536

Abstract

Controversy remains about the role of core side-chain packing in specifying protein structure. To investigate the influence of core packing on the oligomeric structure of a coiled coil, we engineered a GCN4 leucine zipper mutant that switches from two to three strands upon binding the hydrophobic ligands cyclohexane and benzene. In solution these ligands increased the apparent thermal stability and the oligomerization order of the mutant leucine zipper. The crystal structure of the peptide-benzene complex shows a single benzene molecule bound at the engineered site in the core of the trimer. These results indicate that coiled coils are well-suited to function as molecular switches and emphasize that core packing is an important determinant of oligomerization specificity.

Secondary reference #1

• Title: Crystal structure of an isoleucine-Zipper trimer.
• Authors: P.B.Harbury, P.S.Kim, T.Alber.
• Ref.: Nature, 1994, 371, 80-83.
• PubMed id: 8072533

Secondary reference #2

• Title: A switch between two-, Three-, And four-Stranded coiled coils in gcn4 leucine zipper mutants.
• Authors: P.B.Harbury, T.Zhang, P.S.Kim, T.Alber.
• Ref.: Science, 1993, 262, 1401-1407. [DOI no: 10.1126/science.8248779]
• PubMed id: 8248779

Secondary reference #3

• Title: X-Ray structure of the gcn4 leucine zipper, A two-Stranded, Parallel coiled coil.
• Authors: E.K.O'Shea, J.D.Klemm, P.S.Kim, T.Alber.
• Ref.: Science, 1991, 254, 539-544. [DOI no: 10.1126/science.1948029]
• PubMed id: 1948029