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DNA-binding protein PDB-id
1sso
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62 a.a.
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PDB id: 1sso
Name: DNA-binding protein
Title: Solution structure and DNA-binding properties of a thermostable protein from the archaeon sulfolobus solfataricus

Structure:
Sso7d. Chain: a. Engineered: yes

Source:
Sulfolobus solfataricus. Organism_taxid: 2287

UniProt:
P61991 (DN71_SULSO) Pfam   ArchSchema ?
Seq: 64 a.a.
Struc: 62 a.a.
Key:    PfamA domain
 Secondary structure  CATH domain

Resolution:
not givenÅ

NMR structure:
1 models

Authors:
H.Baumann,S.Knapp,T.Lundback,R.Ladenstein,T.Hard

Key ref:
H.Baumann et al. (1994). Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricus.. Nat Struct Biol, 1, 808-819. [PubMed id: 7634092] [DOI: 10.1038/nsb1194-808]

Date:
31-Mar-95

Release date:
08-May-95
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    Key reference    
 
 
DOI no: 10.1038/nsb1194-808 Nat Struct Biol 1:808-819 (1994)
PubMed id: 7634092  
 
 
Solution structure and DNA-binding properties of a thermostable protein from the archaeon Sulfolobus solfataricus.
H.Baumann, S.Knapp, T.Lundbäck, R.Ladenstein, T.Härd.
 
  ABSTRACT  
 
The archaeon Sulfolobus solfataricus expresses large amounts of a small basic protein, Sso7d, which was previously identified as a DNA-binding protein possibly involved in compaction of DNA. We have determined the solution structure of Sso7d. The protein consists of a triple-stranded anti-parallel beta-sheet onto which an orthogonal double-stranded beta-sheet is packed. This topology is very similar to that found in eukaryotic Src homology-3 (SH3) domains. Sso7d binds strongly (Kd < 10 microM) to double-stranded DNA and protects it from thermal denaturation. In addition, we note that epsilon-mono-methylation of lysine side chains of Sso7d is governed by cell growth temperatures, suggesting that methylation is related to the heat-shock response.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
18064401 C.D.Hardy, and P.K.Martin (2008).
Biochemical characterization of DNA-binding proteins from Pyrobaculum aerophilum and Aeropyrum pernix.
  Extremophiles, 12, 235-246.  
18096617 L.Guo, Y.Feng, Z.Zhang, H.Yao, Y.Luo, J.Wang, and L.Huang (2008).
Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea.
  Nucleic Acids Res, 36, 1129-1137.
PDB code: 2jtm
17243156 G.Renzone, R.M.Vitale, A.Scaloni, M.Rossi, P.Amodeo, and A.Guagliardi (2007).
Structural characterization of the functional regions in the archaeal protein Sso7d.
  Proteins, 67, 189-197.  
17340638 R.Consonni, I.Arosio, T.Recca, P.Fusi, and L.Zetta (2007).
Structural determinants responsible for the thermostability of Sso7d and its single point mutants.
  Proteins, 67, 766-775.
PDB code: 2cvr
15673717 A.Napoli, A.Valenti, V.Salerno, M.Nadal, F.Garnier, M.Rossi, and M.Ciaramella (2005).
Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus.
  Nucleic Acids Res, 33, 564-576.  
16148304 J.Eichler, and M.W.Adams (2005).
Posttranslational protein modification in Archaea.
  Microbiol Mol Biol Rev, 69, 393-425.  
14745831 A.Brehm, K.R.Tufteland, R.Aasland, and P.B.Becker (2004).
The many colours of chromodomains.
  Bioessays, 26, 133-140.  
15317021 A.Merlino, G.Graziano, and L.Mazzarella (2004).
Structural and dynamic effects of alpha-helix deletion in Sso7d: implications for protein thermal stability.
  Proteins, 57, 692-701.  
14563841 H.Lou, Z.Duan, X.Huo, and L.Huang (2004).
Modulation of hyperthermophilic DNA polymerase activity by archaeal chromatin proteins.
  J Biol Chem, 279, 127-132.  
14973201 Y.Wang, D.E.Prosen, L.Mei, J.C.Sullivan, M.Finney, and P.B.Vander Horn (2004).
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro.
  Nucleic Acids Res, 32, 1197-1207.  
14651642 R.Guo, H.Xue, and L.Huang (2003).
Ssh10b, a conserved thermophilic archaeal protein, binds RNA in vivo.
  Mol Microbiol, 50, 1605-1615.  
  15803646 A.Guagliardi, L.Cerchia, and M.Rossi (2002).
The Sso7d protein of Sulfolobus solfataricus: in vitro relationship among different activities.
  Archaea, 1, 87-93.  
12060682 A.Napoli, Y.Zivanovic, C.Bocs, C.Buhler, M.Rossi, P.Forterre, and M.Ciaramella (2002).
DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus.
  Nucleic Acids Res, 30, 2656-2662.  
11711548 H.Delbrück, G.Ziegelin, E.Lanka, and U.Heinemann (2002).
An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4.
  J Biol Chem, 277, 4191-4198.
PDB codes: 1igq 1igu
18762890 X.Chen, R.Guo, L.Huang, and R.Hong (2002).
Evolutionary conservation and DNA binding properties of the Ssh7 proteins fromSulfolobus shibatae.
  Sci China C Life Sci, 45, 583-592.  
11782293 V.Agrawal, and R.K.Kishan (2001).
Functional evolution of two subtly different (similar) folds.
  BMC Struct Biol, 1, 5.  
10861939 S.D'Auria, P.Herman, J.R.Lakowicz, F.Tanfani, E.Bertoli, G.Manco, and M.Rossi (2000).
The esterase from the thermophilic eubacterium Bacillus acidocaldarius: structural-functional relationship and comparison with the esterase from the hyperthermophilic archaeon Archaeoglobus fulgidus.
  Proteins, 40, 473-481.  
  10049378 A.Napoli, J.van der Oost, C.W.Sensen, R.L.Charlebois, M.Rossi, and M.Ciaramella (1999).
An Lrp-like protein of the hyperthermophilic archaeon Sulfolobus solfataricus which binds to its own promoter.
  J Bacteriol, 181, 1474-1480.  
  10585967 P.Hindmarsh, and J.Leis (1999).
Retroviral DNA integration.
  Microbiol Mol Biol Rev, 63, 836.  
9671520 F.Catanzano, G.Graziano, P.Fusi, P.Tortora, and G.Barone (1998).
Differential scanning calorimetry study of the thermodynamic stability of some mutants of Sso7d from Sulfolobus solfataricus.
  Biochemistry, 37, 10493-10498.  
9665172 P.Agback, H.Baumann, S.Knapp, R.Ladenstein, and T.Härd (1998).
Architecture of nonspecific protein-DNA interactions in the Sso7d-DNA complex.
  Nat Struct Biol, 5, 579-584.
PDB code: 1bbx
9580681 P.López-García, S.Knapp, R.Ladenstein, and P.Forterre (1998).
In vitro DNA binding of the archaeal protein Sso7d induces negative supercoiling at temperatures typical for thermophilic growth.
  Nucleic Acids Res, 26, 2322-2328.  
  9573250 R.A.Lutzke, and R.H.Plasterk (1998).
Structure-based mutational analysis of the C-terminal DNA-binding domain of human immunodeficiency virus type 1 integrase: critical residues for protein oligomerization and DNA binding.
  J Virol, 72, 4841-4848.  
9593201 S.Knapp, P.T.Mattson, P.Christova, K.D.Berndt, A.Karshikoff, M.Vihinen, C.I.Smith, and R.Ladenstein (1998).
Thermal unfolding of small proteins with SH3 domain folding pattern.
  Proteins, 31, 309-319.  
9220960 E.Mombelli, M.Afshar, P.Fusi, M.Mariani, P.Tortora, J.P.Connelly, and R.Lange (1997).
The role of phenylalanine 31 in maintaining the conformational stability of ribonuclease P2 from Sulfolobus solfataricus under extreme conditions of temperature and pressure.
  Biochemistry, 36, 8733-8742.  
9171360 L.J.Ball, N.V.Murzina, R.W.Broadhurst, A.R.Raine, S.J.Archer, F.J.Stott, A.G.Murzin, P.B.Singh, P.J.Domaille, and E.D.Laue (1997).
Structure of the chromatin binding (chromo) domain from mouse modifier protein 1.
  EMBO J, 16, 2473-2481.
PDB code: 1ap0
  9416608 T.Lazaridis, I.Lee, and M.Karplus (1997).
Dynamics and unfolding pathways of a hyperthermophilic and a mesophilic rubredoxin.
  Protein Sci, 6, 2589-2605.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.