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PDBsum entry 1soo
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References listed in PDB file
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Key reference
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Title
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The mode of action and the structure of a herbicide in complex with its target: binding of activated hydantocidin to the feedback regulation site of adenylosuccinate synthetase.
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Authors
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R.Fonné-Pfister,
P.Chemla,
E.Ward,
M.Girardet,
K.E.Kreuz,
R.B.Honzatko,
H.J.Fromm,
H.P.Schär,
M.G.Grütter,
S.W.Cowan-Jacob.
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Ref.
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Proc Natl Acad Sci U S A, 1996,
93,
9431-9436.
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PubMed id
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Abstract
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(+)-Hydantocidin, a recently discovered natural spironucleoside with potent
herbicidal activity, is shown to be a proherbicide that, after phosphorylation
at the 5' position, inhibits adenylosuccinate synthetase, an enzyme involved in
de novo purine synthesis. The mode of binding of hydantocidin 5'-monophosphate
to the target enzyme was analyzed by determining the crystal structure of the
enzyme-inhibitor complex at 2.6-A resolution. It was found that adenylosuccinate
synthetase binds the phosphorylated compound in the same fashion as it does
adenosine 5'-monophosphate, the natural feedback regulator of this enzyme. This
work provides the first crystal structure of a herbicide-target complex reported
to date.
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Secondary reference #1
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Title
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Refined crystal structures of unligated adenylosuccinate synthetase from escherichia coli.
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Authors
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M.M.Silva,
B.W.Poland,
C.R.Hoffman,
H.J.Fromm,
R.B.Honzatko.
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Ref.
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J Mol Biol, 1995,
254,
431-446.
[DOI no: ]
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PubMed id
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Figure 1.
Figure 1. Amino acid sequence used in the refinement
of unligated crystal structures of the synthetase.
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Figure 8.
Figure 8. Identification of the active site of the synthetase (top) by the location of IMP and GDP in a preliminary
crystal structure, (middle) by directed mutation, and (bottom) by the mapping of conserved residues of synthetases from
bacteria, yeast and mammals. Drawing by MOLSCRIPT (Kraulis, 1991).
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The above figures are
reproduced from the cited reference
with permission from Elsevier
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Secondary reference #2
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Title
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Crystal structure of adenylosuccinate synthetase from escherichia coli. Evidence for convergent evolution of gtp-Binding domains.
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Authors
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B.W.Poland,
M.M.Silva,
M.A.Serra,
Y.Cho,
K.H.Kim,
E.M.Harris,
R.B.Honzatko.
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Ref.
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J Biol Chem, 1993,
268,
25334-25342.
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PubMed id
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Secondary reference #3
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Title
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Preliminary X-Ray crystallographic study of adenylosuccinate synthetase from escherichia coli.
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Authors
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M.A.Serra,
M.B.Bass,
H.J.Fromm,
R.B.Honzatko.
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Ref.
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J Mol Biol, 1988,
200,
753-754.
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PubMed id
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