PDBsum entry: 1slt

Lectin

PDB-id

1slt

Contents

Description

Header details

Protein chains

Ligands

NDG-GAL ×2

Metal ions

_CL

Waters ×154

* Residue conservation analysis

Tools

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PDB id:

1slt

Name:

Lectin

Title:

Structure of s-lectin, a developmentally regulated vertebrate beta-galactoside binding protein

Structure:

Bovine galectin-1. Chain: a, b. Engineered: yes

Source:

Bos taurus. Cow. Organism_taxid: 9913. Tissue: spleen

UniProt:

Chains A, B: P11116 (LEG1_BOVIN)

Seq:

135 a.a.

Struc:

133 a.a.*

Key:		PfamA domain
		Secondary structure			CATH domain

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Resolution:

1.90Å

R-factor:

0.167

Authors:

D.-I.Liao,O.Herzberg

Key ref:

D.I.Liao et al. (1994). Structure of S-lectin, a developmentally regulated vertebrate beta-galactoside-binding protein.. Proc Natl Acad Sci U S A, 91, 1428-1432. [PubMed id: 8108426] [DOI: 10.1073/pnas.91.4.1428]

Date:

20-Oct-93

Release date:

31-Jan-94

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CATH

SCOP

FSSP

HSSP

PDBSWS

PQS

ProSAT

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Clefts

Surface

Key reference

DOI no: 10.1073/pnas.91.4.1428 Proc Natl Acad Sci U S A 91:1428-1432 (1994)

PubMed id: 8108426

Structure of S-lectin, a developmentally regulated vertebrate beta-galactoside-binding protein.

D.I.Liao, G.Kapadia, H.Ahmed, G.R.Vasta, O.Herzberg.

ABSTRACT

The crystal structure of a 14-kDa bovine spleen S-lectin complexed with the disaccharide N-acetyllactosamine at 1.9-A resolution reveals a surprising structural relationship to legume lectins, despite the lack of sequence homology. Two monomers associate to form an extended beta-sandwich, each with the same jelly roll topology typical of legume lectins but with dramatically trimmed loops and with different dimer association. Each monomer binds one N-acetyllactosamine molecule in a topologically and spatially different site than that of legume lectins. The carbohydrate-binding site provides an unprecedented paradigm for carbohydrate binding, with a unique network of salt bridges. The specificity for beta-galactose arises from intricate interactions that constrain the position of the O4 atom.

Literature references that cite this PDB file's key reference

PubMed id Reference

19444247 G.R.Vasta (2009).
Roles of galectins in infection.

Nat Rev Microbiol, 7, 424-438.

18763034 H.Ahmed, S.J.Du, and G.R.Vasta (2009).
Knockdown of a galectin-1-like protein in zebrafish (Danio rerio) causes defects in skeletal muscle development.

Glycoconj J, 26, 277-283.

19432560 M.C.Miller, I.V.Nesmelova, D.Platt, A.Klyosov, and K.H.Mayo (2009).
The carbohydrate-binding domain on galectin-1 is more extensive for a complex glycan than for simple saccharides: implications for galectin-glycan interactions at the cell surface.

Biochem J, 421, 211-221.

19756298 R.D.Cummings (2009).
The repertoire of glycan determinants in the human glycome.

Mol Biosyst, 5, 1087-1104.

18320588 D.Zhou, H.Ge, J.Sun, Y.Gao, M.Teng, and L.Niu (2008).
Crystal structure of the C-terminal conserved domain of human GRP, a galectin-related protein, reveals a function mode different from those of galectins.

Proteins, 71, 1582-1588.

PDB code: 3b9c

18457568 E.M.Rapoport, O.V.Kurmyshkina, and N.V.Bovin (2008).
Mammalian galectins: structure, carbohydrate specificity, and functions.

Biochemistry (Mosc), 73, 393-405.

18607388 L.A.Earl, and L.G.Baum (2008).
CD45 glycosylation controls T-cell life and death.

Immunol Cell Biol, 86, 608-615.

18433051 M.A.Wälti, S.Thore, M.Aebi, and M.Künzler (2008).
Crystal structure of the putative carbohydrate recognition domain of human galectin-related protein.

Proteins, 72, 804-808.

PDB code: 2jj6

18520064 M.Tamura, K.Kasai, T.Itagaki, T.Nonaka, and Y.Arata (2008).
Identification of a second, non-conserved amino acid that contributes to the unique sugar binding properties of the nematode galectin LEC-1.

Biol Pharm Bull, 31, 1254-1257.

18258591 S.Bi, L.A.Earl, L.Jacobs, and L.G.Baum (2008).
Structural features of galectin-9 and galectin-1 that determine distinct T cell death pathways.

J Biol Chem, 283, 12248-12258.

17978468 Y.Arata, N.Ishii, M.Tamura, T.Nonaka, and K.Kasai (2007).
Identification of the amino acid residue in the nematode galectin LEC-1 responsible for its unique sugar binding property: analysis by combination of site-directed mutagenesis and frontal affinity chromatography.

Biol Pharm Bull, 30, 2012-2017.

16972013 T.Shirai, C.Shionyu-Mitsuyama, T.Ogawa, and K.Muramoto (2006).
Structure based studies of the adaptive diversification process of congerins.

Mol Divers, 10, 567-573.

14997539 M.S.Sujatha, and P.V.Balaji (2004).
Identification of common structural features of binding sites in galactose-specific proteins.

Proteins, 55, 44-65.

15009185 N.G.Than, E.Pick, S.Bellyei, A.Szigeti, O.Burger, Z.Berente, T.Janaky, A.Boronkai, H.Kliman, H.Meiri, H.Bohn, G.N.Than, and B.Sumegi (2004).
Functional analyses of placental protein 13/galectin-13.

Eur J Biochem, 271, 1065-1078.

12554954 D.D.Leonidas, B.M.Swamy, A.G.Bhat, S.R.Inamdar, M.N.Kosmopoulou, E.D.Chrysina, and N.G.Oikonomakos (2003).
Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin.

Acta Crystallogr D Biol Crystallogr, 59, 363-365.

12829506 L.He, S.André, H.C.Siebert, H.Helmholz, B.Niemeyer, and H.J.Gabius (2003).
Detection of ligand- and solvent-induced shape alterations of cell-growth-regulatory human lectin galectin-1 in solution by small angle neutron and x-ray scattering.

Biophys J, 85, 511-524.

14517974 M.G.Ford, T.Weimar, T.Köhli, and R.J.Woods (2003).
Molecular dynamics simulations of galectin-1-oligosaccharide complexes reveal the molecular basis for ligand diversity.

Proteins, 53, 229-240.

11880627 J.S.Richardson, and D.C.Richardson (2002).
Natural beta-sheet proteins use negative design to avoid edge-to-edge aggregation.

Proc Natl Acad Sci U S A, 99, 2754-2759.

11846886 K.Scott, and J.Zhang (2002).
Partial identification by site-directed mutagenesis of a cell growth inhibitory site on the human galectin-1 molecule.

BMC Cell Biol, 3, 3.

10944393 H.Ponstingl, K.Henrick, and J.M.Thornton (2000).
Discriminating between homodimeric and monomeric proteins in the crystalline state.

Proteins, 41, 47-57.

10813833 H.Zhang, K.Huang, Z.Li, L.Banerjei, K.E.Fisher, N.V.Grishin, E.Eisenstein, and O.Herzberg (2000).
Crystal structure of YbaK protein from Haemophilus influenzae (HI1434) at 1.8 A resolution: functional implications.

Proteins, 40, 86-97.

PDB codes: 1dbu 1dbx

10861929 M.A.Bianchet, H.Ahmed, G.R.Vasta, and L.M.Amzel (2000).
Soluble beta-galactosyl-binding lectin (galectin) from toad ovary: crystallographic studies of two protein-sugar complexes.

Proteins, 40, 378-388.

PDB codes: 1a78 1gan

10338018 E.García-Hernández, and A.Hernández-Arana (1999).
Structural bases of lectin-carbohydrate affinities: comparison with protein-folding energetics.

Protein Sci, 8, 1075-1086.

10090286 M.M.Prabu, K.Suguna, and M.Vijayan (1999).
Variability in quaternary association of proteins with the same tertiary fold: a case study and rationalization involving legume lectins.

Proteins, 35, 58-69.

9760227 D.D.Leonidas, E.H.Vatzaki, H.Vorum, J.E.Celis, P.Madsen, and K.R.Acharya (1998).
Structural basis for the recognition of carbohydrates by human galectin-7.

Biochemistry, 37, 13930-13940.

PDB codes: 1bkz 2gal

9558320 F.P.Schwarz, H.Ahmed, M.A.Bianchet, L.M.Amzel, and G.R.Vasta (1998).
Thermodynamics of bovine spleen galectin-1 binding to disaccharides: correlation with structure and its effect on oligomerization at the denaturation temperature.

Biochemistry, 37, 5867-5877.

9521730 R.Y.Yang, P.N.Hill, D.K.Hsu, and F.T.Liu (1998).
Role of the carboxyl-terminal lectin domain in self-association of galectin-3.

Biochemistry, 37, 4086-4092.

9234729 A.Vyakarnam, S.F.Dagher, J.L.Wang, and R.J.Patterson (1997).
Evidence for a role for galectin-1 in pre-mRNA splicing.

Mol Cell Biol, 17, 4730-4737.

9363750 H.C.Siebert, R.Adar, R.Arango, M.Burchert, H.Kaltner, G.Kayser, E.Tajkhorshid, C.W.von der Lieth, R.Kaptein, N.Sharon, J.F.Vliegenthart, and H.J.Gabius (1997).
Involvement of laser photo-CIDNP (chemically induced dynamic nuclear polarization)-reactive amino acid side chains in ligand binding by galactoside-specific lectins in solution.

Eur J Biochem, 249, 27-38.

9057819 H.J.Gabius (1997).
Animal lectins.

Eur J Biochem, 243, 543-576.

9334741 T.C.Umland, L.M.Wingert, S.Swaminathan, W.F.Furey, J.J.Schmidt, and M.Sax (1997).
Structure of the receptor binding fragment HC of tetanus neurotoxin.

Nat Struct Biol, 4, 788-792.

PDB code: 1af9

8855944 M.Cho, and R.D.Cummings (1996).
Characterization of monomeric forms of galectin-1 generated by site-directed mutagenesis.

Biochemistry, 35, 13081-13088.

8885835 M.Muraki, K.Harata, N.Sugita, and K.Sato (1996).
Origin of carbohydrate recognition specificity of human lysozyme revealed by affinity labeling.

Biochemistry, 35, 13562-13567.

PDB codes: 1rex 1rey 1rez

8916925 P.E.Johnson, M.D.Joshi, P.Tomme, D.G.Kilburn, and L.P.McIntosh (1996).
Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy.

Biochemistry, 35, 14381-14394.

PDB codes: 1ulo 1ulp

8774711 R.Adar, and N.Sharon (1996).
Mutational studies of the amino acid residues in the combining site of Erythrina corallodendron lectin.

Eur J Biochem, 239, 668-674.

8706686 S.Rosén, M.Kata, Y.Persson, P.H.Lipniunas, M.Wikström, M.J.Van Den Hondel, J.Van Den Brink, L.Rask, L.O.Hedén, and A.Tunlid (1996).
Molecular characterization of a saline-soluble lectin from a parasitic fungus. Extensive sequence similarities between fungal lectins.

Eur J Biochem, 238, 822-829.

8962033 T.L.Blundell, and N.Srinivasan (1996).
Symmetry, stability, and dynamics of multidomain and multicomponent protein systems.

Proc Natl Acad Sci U S A, 93, 14243-14248.

7869048 L.G.Baum, M.Pang, N.L.Perillo, T.Wu, A.Delegeane, C.H.Uittenbogaart, M.Fukuda, and J.J.Seilhamer (1995).
Human thymic epithelial cells express an endogenous lectin, galectin-1, which binds to core 2 O-glycans on thymocytes and T lymphoblastoid cells.

J Exp Med, 181, 877-887.

8575449 M.Menéndez, M.Gasset, J.Laynez, C.López-Zumel, P.Usobiaga, E.Töpfer-Petersen, and J.J.Calvete (1995).
Analysis of the structural organization and thermal stability of two spermadhesins. Calorimetric, circular dichroic and Fourier-transform infrared spectroscopic studies.

Eur J Biochem, 234, 887-896.

7987210 H.J.Hoppe, and K.B.Reid (1994).
Collectins--soluble proteins containing collagenous regions and lectin domains--and their roles in innate immunity.

Protein Sci, 3, 1143-1158.

7937731 L.Holm, and C.Sander (1994).
Searching protein structure databases has come of age.

Proteins, 19, 165-173.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.