|
|
||
|
|
|
|
|
UniProt functional annotation for P06968 | ||
|
|
|
|
|
|
||
| UniProt code: P06968. |
|
||
| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
|
||
|
||
| Function: | |
This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. {ECO:0000269|PubMed:9261872}. |
|
||
| Catalytic activity: | |
Reaction=dUTP + H2O = diphosphate + dUMP + H(+); Xref=Rhea:RHEA:10248, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:61555, ChEBI:CHEBI:246422; EC=3.6.1.23; Evidence={ECO:0000269|PubMed:9261872}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15208312}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.28 uM for dUTP {ECO:0000269|PubMed:9261872}; Note=kcat is 5.15 s(-1). {ECO:0000269|PubMed:9261872}; |
| Pathway: | |
Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. |
| Subunit: | |
Homotrimer. {ECO:0000269|PubMed:1311056, ECO:0000269|PubMed:15208312, ECO:0000269|PubMed:9261872}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305|PubMed:1311056, ECO:0000305|PubMed:9261872}. |
| Domain: | |
The C-terminal 11 residues (motif 5) is important for function; its removal by trypsin decreases kcat about 40-fold with a less than 2- fold increase in KM for dUTP. {ECO:0000269|PubMed:9261872}. |
| Mass spectrometry: | |
Mass=16296; Method=Electrospray; Evidence={ECO:0000269|PubMed:9261872}; |
| Similarity: | |
Belongs to the dUTPase family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA61993.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:9261872}; Sequence=AAC76664.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:9261872}; Sequence=BAE77652.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:9261872}; Sequence=CAA24897.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:9261872}; Sequence=CAA25859.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305|PubMed:9261872}; |
Annotations taken from UniProtKB at the EBI.