PDBsum entry 1san

DNA binding protein

PDB id: 1san

Contents

Protein chain

62 a.a.

References listed in PDB file

Key reference

• Title: The des(1-6)antennapedia homeodomain: comparison of the nmr solution structure and the DNA-Binding affinity with the intact antennapedia homeodomain.
• Authors: Y.Q.Qian, D.Resendez-Perez, W.J.Gehring, K.Wüthrich.
• Ref.: Proc Natl Acad Sci U S A, 1994, 91, 4091-4095. [DOI no: 10.1073/pnas.91.9.4091]
• PubMed id: 7909611

Abstract

The nuclear magnetic resonance (NMR) solution structure of an N-terminally truncated mutant Antennapedia homeodomain, des(1-6)Antp(C39S), has been determined from 935 nuclear Overhauser effect upper distance constraints and 148 dihedral angle constraints by using the programs DIANA and OPAL. Twenty conformers representing the solution structure of des(1-6)Antp(C39S) have an average root-mean-square distance relative to the mean coordinates of 0.56 A for the backbone atoms of residues 8-59. Comparison with the intact Antp(C39S) homeodomain shows that the two proteins have identical molecular architectures. The removal of the N-terminal residues 1-6, which are flexibly disordered in the intact homeodomain, causes only strictly localized structure variations and does not noticeably affect the adjoining helix I from residues 10-21. The DNA-binding constant of des(1-6)Antp(C39S) is approximately 10-fold reduced relative to the intact Antp(C39S) homeodomain, which can now be attributed to the absence of the previously reported contacts of the N-terminal polypeptide segment of the intact Antp(C39S) homeodomain with the minor groove of the DNA duplex.

Secondary reference #1

• Title: Structure determination of the antp (c39----S) homeodomain from nuclear magnetic resonance data in solution using a novel strategy for the structure calculation with the programs diana, Caliba, Habas and glomsa.
• Authors: P.Güntert, Y.Q.Qian, G.Otting, M.Müller, W.Gehring, K.Wüthrich.
• Ref.: J Mol Biol, 1991, 217, 531-540.
• PubMed id: 1671604

Secondary reference #2

• Title: The structure of the antennapedia homeodomain determined by nmr spectroscopy in solution: comparison with prokaryotic repressors.
• Authors: Y.Q.Qian, M.Billeter, G.Otting, M.Müller, W.J.Gehring, K.Wüthrich.
• Ref.: Cell, 1989, 59, 573-580. [DOI no: 10.1016/0092-8674(89)90040-8]
• PubMed id: 2572329

Secondary reference #3

• Title: Determination of the three-Dimensional structure of the antennapedia homeodomain from drosophila in solution by 1h nuclear magnetic resonance spectroscopy.
• Authors: M.Billeter, Y.Qian, G.Otting, M.Müller, W.J.Gehring, K.Wüthrich.
• Ref.: J Mol Biol, 1990, 214, 183-197.
• PubMed id: 2164583