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UniProt functional annotation for P08692 | ||
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| UniProt code: P08692. |
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| Organism: | |
Escherichia coli. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in resistance to arsenate (PubMed:3021763, PubMed:8003492, PubMed:7577935). Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)] (PubMed:8003492, PubMed:7577935, PubMed:14592722). The resulting arsenite is then extruded from the cell via the ArsAB transport system (Probable). {ECO:0000269|PubMed:14592722, ECO:0000269|PubMed:3021763, ECO:0000269|PubMed:7577935, ECO:0000269|PubMed:8003492, ECO:0000305}. |
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| Catalytic activity: | |
Reaction=[glutaredoxin]-dithiol + arsenate + glutathione + 2 H(+) = arsenite + glutathionyl-S-S-[glutaredoxin] + H2O; Xref=Rhea:RHEA:22016, Rhea:RHEA-COMP:10729, Rhea:RHEA-COMP:16283, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:29950, ChEBI:CHEBI:48597, ChEBI:CHEBI:57925, ChEBI:CHEBI:146199; EC=1.20.4.1; Evidence={ECO:0000269|PubMed:14592722, ECO:0000269|PubMed:7577935, ECO:0000269|PubMed:8003492}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22017; Evidence={ECO:0000269|PubMed:14592722, ECO:0000269|PubMed:7577935, ECO:0000269|PubMed:8003492}; |
| Activity regulation: | |
Inhibited by the thiol reagents iodoacetate (IAA) and N-ethylmaleimide (NEM) (PubMed:7577935). Activity is rapidly inactivated by the histidine-modifying reagent diethylpyrocarbonate (DEPC) (PubMed:8969183). {ECO:0000269|PubMed:7577935, ECO:0000269|PubMed:8969183}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=8 mM for arsenate {ECO:0000269|PubMed:8003492}; KM=15.2 mM for arsenate {ECO:0000269|PubMed:14592722}; KM=32.9 nM for glutaredoxin Grx2 {ECO:0000269|PubMed:14592722}; Note=kcat is 0.53 sec(-1) with arsenate as substrate. kcat is 0.218 sec(-1) with Grx2 as substrate. {ECO:0000269|PubMed:14592722}; pH dependence: Optimum pH is 6.3-6.8. {ECO:0000269|PubMed:8003492}; |
| Subunit: | |
Monomer in solution. {ECO:0000269|PubMed:1703401}. |
| Similarity: | |
Belongs to the ArsC family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.