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UniProt functional annotation for Q00001 | ||
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| UniProt code: Q00001. |
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| Organism: | |
Aspergillus aculeatus. |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Circumdati. |
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| Function: | |
Pectinolytic enzymes consist of four classes of enzymes: pectine lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Has a positive effect in the apple hot-mash liquefaction process. Hydrolyzes alpha-D-galacturonopyranosyl-(1,2)- alpha-L-rhamnopyranosyl linkages in the backbone of the hairy regions of pectins. {ECO:0000269|PubMed:9464254}. |
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| Catalytic activity: | |
Reaction=Endohydrolysis of alpha-D-GalA-(1->2)-alpha-L-Rha glycosidic bond in the rhamnogalacturonan I backbone with initial inversion of anomeric configuration releasing oligosaccharides with beta-D-GalA at the reducing end.; EC=3.2.1.171; |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 3.5. Unstable above pH 6.0.; Temperature dependence: Optimum temperature is 30-50 degrees Celsius.; |
| Subcellular location: | |
Secreted. |
| Ptm: | |
The N-terminus is blocked. |
| Ptm: | |
N-glycosylated and may also be O-glycosylated. |
| Similarity: | |
Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.