PDBsum entry 1rlc

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Lyase(carbon-carbon) PDB id
Protein chains
441 a.a. *
123 a.a. *
* Residue conservation analysis

References listed in PDB file
Key reference
Title Crystal structure of the unactivated ribulose 1,5-Bisphosphate carboxylase/oxygenase complexed with a transition state analog, 2-Carboxy-D-Arabinitol 1,5-Bisphosphate.
Authors K.Y.Zhang, D.Cascio, D.Eisenberg.
Ref. Protein Sci, 1994, 3, 64-69. [DOI no: 10.1002/pro.5560030109]
PubMed id 8142899
Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 86%.
The crystal structure of unactivated ribulose 1,5-bisphosphate carboxylase/oxygenase from Nicotiana tabacum complexed with a transition state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate, was determined to 2.7 A resolution by X-ray crystallography. The transition state analog binds at the active site in an extended conformation. As compared to the binding of the same analog in the activated enzyme, the analog binds in a reverse orientation. The active site Lys 201 is within hydrogen bonding distance of the carboxyl oxygen of the analog. Loop 6 (residues 330-339) remains open and flexible upon binding of the analog in the unactivated enzyme, in contrast to the closed and ordered loop 6 in the activated enzyme complex. The transition state analog is exposed to solvent due to the open conformation of loop 6.
Secondary reference #1
Title Crystal structure of the unactivated form of ribulose-1,5-Bisphosphate carboxylase/oxygenase from tobacco refined at 2.0-A resolution.
Authors P.M.Curmi, D.Cascio, R.M.Sweet, D.Eisenberg, H.Schreuder.
Ref. J Biol Chem, 1992, 267, 16980-16989.
PubMed id 1512238
Secondary reference #2
Title Tertiary structure of plant rubisco: domains and their contacts.
Authors M.S.Chapman, S.W.Suh, P.M.Curmi, D.Cascio, W.W.Smith, D.S.Eisenberg.
Ref. Science, 1988, 241, 71-74. [DOI no: 10.1126/science.3133767]
PubMed id 3133767
Full text Abstract
Secondary reference #3
Title Sliding-Layer conformational change limited by the quaternary structure of plant rubisco.
Authors M.S.Chapman, S.W.Suh, D.Cascio, W.W.Smith, D.Eisenberg.
Ref. Nature, 1987, 329, 354-356.
PubMed id 3627277
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