PDBsum entry 1rgw

Structural protein

PDB id: 1rgw

Contents

Protein chain

85 a.a. *

* Residue conservation analysis

PDB id:

1rgw

Name:

Structural protein

Title:

Solution structure of zasp's pdz domain

Structure:

Zasp protein. Chain: a. Fragment: pdz domain. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Tissue: muscle. Gene: zasp. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

Y.Au,R.A.Atkinson,A.Pallavicini,C.Joseph,S.R.Martin,F.W.Muskett, R.Guerrini,G.Faulkner,A.Pastore

Key ref:

Y.Au et al. (2004). Solution structure of ZASP PDZ domain; implications for sarcomere ultrastructure and enigma family redundancy. Structure, 12, 611-622. PubMed id: 15062084 DOI: 10.1016/j.str.2004.02.019

Date:

13-Nov-03 Release date: 13-Apr-04

Protein chain

O75112  (LDB3_HUMAN) -  LIM domain-binding protein 3 from Homo sapiens

Seq: 727 a.a.

Struc: 85 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.str.2004.02.019

Structure 12:611-622 (2004)

PubMed id: 15062084

Solution structure of ZASP PDZ domain; implications for sarcomere ultrastructure and enigma family redundancy.

Y.Au, R.A.Atkinson, R.Guerrini, G.Kelly, C.Joseph, S.R.Martin, F.W.Muskett, A.Pallavicini, G.Faulkner, A.Pastore.

ABSTRACT

Z band alternately spliced PDZ-containing protein (ZASP) is a sarcomere Z disk protein expressed in human cardiac and skeletal muscle that is thought to be involved in a dominant familial dilated cardiomyopathy. The N-terminal PDZ domain of ZASP interacts with the C terminus of alpha-actinin-2, the major component of the Z disk, probably by forming a ternary complex with titin Z repeats. We have determined the structure of ZASP PDZ by NMR and showed that it is a classical class 1 PDZ domain that recognizes the carboxy-terminal sequence of an alpha-actinin-2 calmodulin-like domain with micromolar affinity. We also characterized the role of each component in the ternary complex ZASP/alpha-actinin-2/titin, showing that the alpha-actinin-2/ZASP PDZ interaction involves a binding surface distinct from that recognized by the titin Z repeats. ZASP PDZ structure was used to model other members of the enigma family by homology and to predict their abilities to bind alpha-actinin-2.

Selected figure(s)

Figure 1.
Figure 1. Diagram of the Sarcomere and Domain Architecture(A) Schematic representation of the sarcomere. The position of the Z disk and of the M line is indicated. A single molecule titin filament (indicated in green) connects the Z disk to the M line. In the Z disk, a-actinin-2 (in yellow) forms transversal connections between actin filaments (in red) and is thought to bind ZASP (in blue).(B) Domain architecture of the titin region localized in the Z disk, a-actinin-2, and ZASP (variant 3).

The above figure is reprinted by permission from Cell Press: Structure (2004, 12, 611-622) copyright 2004.

Figure was selected by an automated process.