UniProt functional annotation for Q5EBH1



	UniProt functional annotation for Q5EBH1

UniProt code: Q5EBH1.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing (By similarity). May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis. {ECO:0000250, ECO:0000269|PubMed:11864565}.

Subunit: Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS (PubMed:9488663, PubMed:11857081, PubMed:11864565, PubMed:18596699). Interacts with KRAS (PubMed:11857081, PubMed:11864565). Probably interacts with Ras-like GTPases RRAS, MRAS, RAP1B, RAP2A and RALA (PubMed:11857081). Interacts with RRAS2 (By similarity). Can self-associate (PubMed:11857081). Interacts with RSSF1 isoform A (PubMed:11857081). The RSSF1 isoform A- RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS (PubMed:11864565, PubMed:11857081). Isoform 2 interacts with activated RAP1A and ITGAL/LFA-1 (By similarity). Binds STK4/MST1, inhibiting STK4/MST1 autoactivation (PubMed:11864565). {ECO:0000250|UniProtKB:Q8WWW0, ECO:0000269|PubMed:11857081, ECO:0000269|PubMed:11864565, ECO:0000269|PubMed:18596699, ECO:0000269|PubMed:9488663}.

Subcellular location: Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A (By similarity). {ECO:0000250}.

Sequence caution: Sequence=BAD21397.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing (By similarity). May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis. {ECO:0000250, ECO:0000269\|PubMed:11864565}.


Subunit:		Interacts directly with activated HRAS; a RASSF5-STK4/MST1 complex probably associates with activated HRAS (PubMed:9488663, PubMed:11857081, PubMed:11864565, PubMed:18596699). Interacts with KRAS (PubMed:11857081, PubMed:11864565). Probably interacts with Ras-like GTPases RRAS, MRAS, RAP1B, RAP2A and RALA (PubMed:11857081). Interacts with RRAS2 (By similarity). Can self-associate (PubMed:11857081). Interacts with RSSF1 isoform A (PubMed:11857081). The RSSF1 isoform A- RSSF5 heterodimer probably mediates the association of RSSF1 with HRAS (PubMed:11864565, PubMed:11857081). Isoform 2 interacts with activated RAP1A and ITGAL/LFA-1 (By similarity). Binds STK4/MST1, inhibiting STK4/MST1 autoactivation (PubMed:11864565). {ECO:0000250\|UniProtKB:Q8WWW0, ECO:0000269\|PubMed:11857081, ECO:0000269\|PubMed:11864565, ECO:0000269\|PubMed:18596699, ECO:0000269\|PubMed:9488663}.
Subcellular location:		Cytoplasm {ECO:0000250}. Cytoplasm, cytoskeleton {ECO:0000250}. Note=Isoform 2 is mainly located in the perinuclear region of unstimulated primary T-cells. Upon stimulation translocates to the leading edge and colocalizes with ITGAL/LFA-1 in the peripheral zone of the immunological synapse. Isoform 2 is localized to growing microtubules in vascular endothelial cells and is dissociated from microtubules by activated RAP1A (By similarity). {ECO:0000250}.
Sequence caution:		Sequence=BAD21397.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};