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PDBsum entry 1rea
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protein ligands links
DNA binding protein PDB id
1rea
Contents
Protein chain
304 a.a.* *
Ligands
ADP
* Residue conservation analysis
* C-alpha coords only
PDB id:
1rea
Name: DNA binding protein
Title: Structure of the reca protein-adp complex
Structure: Rec a. Chain: a. Engineered: yes
Source: Escherichia coli. Organism_taxid: 562
Resolution:
2.70Å     R-factor:   0.223    
Authors: R.M.Story,T.A.Steitz
Key ref: R.M.Story and T.A.Steitz (1992). Structure of the recA protein-ADP complex. Nature, 355, 374-376. PubMed id: 1731253
Date:
19-Dec-91     Release date:   31-Oct-93    
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P0A7G6  (RECA_ECOLI) -  Protein RecA from Escherichia coli (strain K12)
Seq:
Struc: 		353 a.a.
304 a.a.
Key:    PfamA domain  Secondary structure

 

 
    Key reference    
 
 
Nature 355:374-376 (1992)
PubMed id: 1731253  
 
 
Structure of the recA protein-ADP complex.
R.M.Story, T.A.Steitz.
 
  ABSTRACT  
 
The recA protein catalyses the ATP-driven homologous pairing and strand exchange of DNA molecules. It is an allosteric enzyme: the ATPase activity is DNA-dependent, and ATP-bound recA protein has a high affinity for DNA, whereas the ADP-bound form has a low affinity. In the absence of ATP hydrolysis, recA protein can still promote homologous pairing, apparently through the formation of a triple-stranded intermediate. The exact role of ATP hydrolysis is not clear, but it presumably drives the triplex intermediate towards products. Here we determine the position of bound ADP diffused into the recA crystal. We show that only the phosphates are bound in the same way as in other NTPases containing the G/AXXXXGKT/S motif. We propose that recA protein may change its conformation upon ATP hydrolysis in a manner analogous to one such protein, the p21 protein from the ras oncogene. A model is presented to account for the allosteric stimulation of DNA binding by ATP. The mechanism by which nucleoside triphosphate hydrolysis is coupled to the binding of another ligand in recA protein and p21 may be typical of the large class of NTPases containing this conserved motif.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21282052 A.Y.Lyubimov, M.Strycharska, and J.M.Berger (2011).
The nuts and bolts of ring-translocase structure and mechanism.
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20386943 C.Carra, and F.A.Cucinotta (2011).
Binding selectivity of RecA to a single stranded DNA, a computational approach.
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21391900 J.Strohmeier, I.Hertel, U.Diederichsen, M.G.Rudolph, and D.Klostermeier (2011).
Changing nucleotide specificity of the DEAD-box helicase Hera abrogates communication between the Q-motif and the P-loop.
  Biol Chem, 392, 357-369.
PDB codes: 3mwj 3mwk 3mwl 3nbf 3nej
21458462 V.E.Galkin, R.L.Britt, L.B.Bane, X.Yu, M.M.Cox, and E.H.Egelman (2011).
Two modes of binding of DinI to RecA filament provide a new insight into the regulation of SOS response by DinI protein.
  J Mol Biol, 408, 815-824.  
20351255 A.K.Satapathy, A.B.Kochaniak, S.Mukherjee, D.J.Crampton, A.van Oijen, and C.C.Richardson (2010).
Residues in the central beta-hairpin of the DNA helicase of bacteriophage T7 are important in DNA unwinding.
  Proc Natl Acad Sci U S A, 107, 6782-6787.  
21153518 C.Basavannacharya, P.R.Moody, T.Munshi, N.Cronin, N.H.Keep, and S.Bhakta (2010).
Essential residues for the enzyme activity of ATP-dependent MurE ligase from Mycobacterium tuberculosis.
  Protein Cell, 1, 1011-1022.
PDB code: 2xja
  20798837 R.K.Chittela, and J.K.Sainis (2010).
Plant DNA recombinases: a long way to go.
  J Nucleic Acids, 2010, 0.  
19910465 R.L.Britt, N.Haruta, S.L.Lusetti, S.Chitteni-Pattu, R.B.Inman, and M.M.Cox (2010).
Disassembly of Escherichia coli RecA E38K/DeltaC17 nucleoprotein filaments is required to complete DNA strand exchange.
  J Biol Chem, 285, 3211-3226.  
19066203 A.A.Grigorescu, J.H.Vissers, D.Ristic, Y.Z.Pigli, T.W.Lynch, C.Wyman, and P.A.Rice (2009).
Inter-subunit interactions that coordinate Rad51's activities.
  Nucleic Acids Res, 37, 557-567.  
19430763 A.M.Brandt, W.Raksajit, P.Mulo, A.Incharoensakdi, T.A.Salminen, and P.Mäenpää (2009).
Transcriptional regulation and structural modeling of the FutC subunit of an ABC-type iron transporter in Synechocystis sp. strain PCC 6803.
  Arch Microbiol, 191, 561-570.  
19338667 C.D.Lee, and T.F.Wang (2009).
The N-terminal domain of Escherichia coli RecA have multiple functions in promoting homologous recombination.
  J Biomed Sci, 16, 37.  
19050012 D.Klostermeier, and M.G.Rudolph (2009).
A novel dimerization motif in the C-terminal domain of the Thermus thermophilus DEAD box helicase Hera confers substantial flexibility.
  Nucleic Acids Res, 37, 421-430.
PDB codes: 3eaq 3ear 3eas
19555451 J.E.Long, N.Renzette, and S.J.Sandler (2009).
Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12.
  Mol Microbiol, 73, 226-239.  
19027026 J.N.Farb, and S.W.Morrical (2009).
Role of allosteric switch residue histidine 195 in maintaining active-site asymmetry in presynaptic filaments of bacteriophage T4 UvsX recombinase.
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19666566 M.M.Cox (2009).
A new look at the human Rad51 protein.
  Proc Natl Acad Sci U S A, 106, 13147-13148.  
19589129 S.H.Ling, Z.Cheng, and H.Song (2009).
Structural aspects of RNA helicases in eukaryotic mRNA decay.
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19544044 V.Kos, and R.C.Ford (2009).
The ATP-binding cassette family: a structural perspective.
  Cell Mol Life Sci, 66, 3111-3126.  
19033358 X.P.Zhang, V.E.Galkin, X.Yu, E.H.Egelman, and W.D.Heyer (2009).
Loop 2 in Saccharomyces cerevisiae Rad51 protein regulates filament formation and ATPase activity.
  Nucleic Acids Res, 37, 158-171.  
19074952 Y.H.Lo, K.L.Tsai, Y.J.Sun, W.T.Chen, C.Y.Huang, and C.D.Hsiao (2009).
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.
  Nucleic Acids Res, 37, 804-814.
PDB codes: 2vye 2vyf
19295907 Y.W.Chang, T.P.Ko, C.D.Lee, Y.C.Chang, K.A.Lin, C.S.Chang, A.H.Wang, and T.F.Wang (2009).
Three new structures of left-handed RADA helical filaments: structural flexibility of N-terminal domain is critical for recombinase activity.
  PLoS ONE, 4, e4890.
PDB codes: 2zub 2zuc 2zud
18755053 A.J.Schoeffler, and J.M.Berger (2008).
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology.
  Q Rev Biophys, 41, 41.  
18057007 D.E.Kainov, E.J.Mancini, J.Telenius, J.Lísal, J.M.Grimes, D.H.Bamford, D.I.Stuart, and R.Tuma (2008).
Structural basis of mechanochemical coupling in a hexameric molecular motor.
  J Biol Chem, 283, 3607-3617.
PDB codes: 2vhc 2vhj 2vhq 2vht 2vhu
19008861 D.Luo, T.Xu, R.P.Watson, D.Scherer-Becker, A.Sampath, W.Jahnke, S.S.Yeong, C.H.Wang, S.P.Lim, A.Strongin, S.G.Vasudevan, and J.Lescar (2008).
Insights into RNA unwinding and ATP hydrolysis by the flavivirus NS3 protein.
  EMBO J, 27, 3209-3219.
PDB codes: 2jlq 2jlr 2jls 2jlu 2jlv 2jlw 2jlx 2jly 2jlz
18975973 I.Garcia, and O.C.Uhlenbeck (2008).
Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins.
  Biochemistry, 47, 12562-12573.  
18603529 J.M.Cox, H.Li, E.A.Wood, S.Chitteni-Pattu, R.B.Inman, and M.M.Cox (2008).
Defective Dissociation of a "Slow" RecA Mutant Protein Imparts an Escherichia coli Growth Defect.
  J Biol Chem, 283, 24909-24921.  
18386081 J.Petersen, C.J.Mitchell, K.Fisher, and D.J.Lowe (2008).
Structural basis for VO(2+)-inhibition of nitrogenase activity: (B) pH-sensitive inner-sphere rearrangements in the 1H-environment of the metal coordination site of the nitrogenase Fe-protein identified by ENDOR spectroscopy.
  J Biol Inorg Chem, 13, 637-650.  
19020353 J.R.Prabu, G.P.Manjunath, N.R.Chandra, K.Muniyappa, and M.Vijayan (2008).
Functionally important movements in RecA molecules and filaments: studies involving mutation and environmental changes.
  Acta Crystallogr D Biol Crystallogr, 64, 1146-1157.
PDB codes: 2zr0 2zr7 2zr9 2zra 2zrb 2zrc 2zrd 2zre 2zrf 2zrg 2zrh 2zri 2zrj 2zrk 2zrl 2zrm 2zrn 2zro 2zrp
17986459 L.M.Elles, and O.C.Uhlenbeck (2008).
Mutation of the arginine finger in the active site of Escherichia coli DbpA abolishes ATPase and helicase activity and confers a dominant slow growth phenotype.
  Nucleic Acids Res, 36, 41-50.  
18647240 N.D.Thomsen, and J.M.Berger (2008).
Structural frameworks for considering microbial protein- and nucleic acid-dependent motor ATPases.
  Mol Microbiol, 69, 1071-1090.  
18497811 S.C.Kowalczykowski (2008).
Structural biology: snapshots of DNA repair.
  Nature, 453, 463-466.  
18687036 V.B.Rao, and M.Feiss (2008).
The bacteriophage DNA packaging motor.
  Annu Rev Genet, 42, 647-681.  
18042682 X.Zhang, T.Nakashima, Y.Kakuta, M.Yao, I.Tanaka, and M.Kimura (2008).
Crystal structure of an archaeal Ski2p-like protein from Pyrococcus horikoshii OT3.
  Protein Sci, 17, 136-145.
PDB code: 2z41
17255937 A.Yamagata, and J.A.Tainer (2007).
Hexameric structures of the archaeal secretion ATPase GspE and implications for a universal secretion mechanism.
  EMBO J, 26, 878-890.
PDB codes: 2oap 2oaq
17084859 D.N.Frick, S.Banik, and R.S.Rypma (2007).
Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus NS3 helicase: magnesium provides a bridge for ATP to fuel unwinding.
  J Mol Biol, 365, 1017-1032.  
17602667 H.Qiu, and Y.Wang (2007).
Probing adenosine nucleotide-binding proteins with an affinity-labeled nucleotide probe and mass spectrometry.
  Anal Chem, 79, 5547-5556.  
17766252 H.Ren, S.X.Dou, P.Rigolet, Y.Yang, P.Y.Wang, M.Amor-Gueret, and X.G.Xi (2007).
The arginine finger of the Bloom syndrome protein: its structural organization and its role in energy coupling.
  Nucleic Acids Res, 35, 6029-6041.  
17355871 K.A.Satyshur, G.A.Worzalla, L.S.Meyer, E.K.Heiniger, K.G.Aukema, A.M.Misic, and K.T.Forest (2007).
Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility.
  Structure, 15, 363-376.
PDB codes: 2ewv 2eww 2eyu 2gsz
17329376 L.T.Chen, T.P.Ko, Y.C.Chang, K.A.Lin, C.S.Chang, A.H.Wang, and T.F.Wang (2007).
Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins.
  Nucleic Acids Res, 35, 1787-1801.
PDB code: 2dfl
17848989 L.T.Chen, T.P.Ko, Y.W.Chang, K.A.Lin, A.H.Wang, and T.F.Wang (2007).
Structural and functional analyses of five conserved positively charged residues in the L1 and N-terminal DNA binding motifs of archaeal RADA protein.
  PLoS ONE, 2, e858.
PDB code: 2z43
17228330 M.M.Cox (2007).
Motoring along with the bacterial RecA protein.
  Nat Rev Mol Cell Biol, 8, 127-138.  
17324440 T.D.Ziebarth, C.L.Farr, and L.S.Kaguni (2007).
Modular architecture of the hexameric human mitochondrial DNA helicase.
  J Mol Biol, 367, 1382-1391.  
17316710 Y.Bao, and X.Shen (2007).
INO80 subfamily of chromatin remodeling complexes.
  Mutat Res, 618, 18-29.  
17159905 Z.Cheng, D.Muhlrad, M.K.Lim, R.Parker, and H.Song (2007).
Structural and functional insights into the human Upf1 helicase core.
  EMBO J, 26, 253-264.
PDB codes: 2gjk 2gk6 2gk7
16669707 A.Talà, F.Belloni, C.Monaco, A.Lorusso, V.Nassisi, and P.Alifano (2006).
Effects of XeCl UV308 nm laser radiation on survival and mutability of recA-proficient and recA-defective Escherichia coli strains.
  Radiat Res, 165, 532-537.  
16541253 C.Oswald, I.B.Holland, and L.Schmitt (2006).
The motor domains of ABC-transporters. What can structures tell us?
  Naunyn Schmiedebergs Arch Pharmacol, 372, 385-399.  
16427007 D.J.Crampton, S.Mukherjee, and C.C.Richardson (2006).
DNA-induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase.
  Mol Cell, 21, 165-174.  
16935879 I.Donmez, and S.S.Patel (2006).
Mechanisms of a ring shaped helicase.
  Nucleic Acids Res, 34, 4216-4224.  
  17142894 J.M.Caruthers, Y.Hu, and D.B.McKay (2006).
Structure of the second domain of the Bacillus subtilis DEAD-box RNA helicase YxiN.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 62, 1191-1195.
PDB code: 2hjv
16527806 J.M.Cox, S.N.Abbott, S.Chitteni-Pattu, R.B.Inman, and M.M.Cox (2006).
Complementation of one RecA protein point mutation by another. Evidence for trans catalysis of ATP hydrolysis.
  J Biol Chem, 281, 12968-12975.  
16689629 J.P.Erzberger, and J.M.Berger (2006).
Evolutionary relationships and structural mechanisms of AAA+ proteins.
  Annu Rev Biophys Biomol Struct, 35, 93.  
16972168 M.Hoffmann, K.Eitner, M.von Grotthuss, L.Rychlewski, E.Banachowicz, T.Grabarkiewicz, T.Szkoda, and A.Kolinski (2006).
Three dimensional model of severe acute respiratory syndrome coronavirus helicase ATPase catalytic domain and molecular design of severe acute respiratory syndrome coronavirus helicase inhibitors.
  J Comput Aided Mol Des, 20, 305-319.  
16909421 M.Petukhov, D.Lebedev, V.Shalguev, A.Islamov, A.Kuklin, V.Lanzov, and V.Isaev-Ivanov (2006).
Conformational flexibility of RecA protein filament: transitions between compressed and stretched states.
  Proteins, 65, 296-304.  
16258174 M.S.Mitchell, and V.B.Rao (2006).
Functional analysis of the bacteriophage T4 DNA-packaging ATPase motor.
  J Biol Chem, 281, 518-527.  
16648362 R.Krishna, G.P.Manjunath, P.Kumar, A.Surolia, N.R.Chandra, K.Muniyappa, and M.Vijayan (2006).
Crystallographic identification of an ordered C-terminal domain and a second nucleotide-binding site in RecA: new insights into allostery.
  Nucleic Acids Res, 34, 2186-2195.
PDB code: 2g88
16611246 T.Matsui, K.Hogetsu, J.Usukura, T.Sato, T.Kumasaka, Y.Akao, and N.Tanaka (2006).
Structural insight of human DEAD-box protein rck/p54 into its substrate recognition with conformational changes.
  Genes Cells, 11, 439-452.
PDB code: 1vec
15755748 A.Ariza, D.J.Richard, M.F.White, and C.S.Bond (2005).
Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA.
  Nucleic Acids Res, 33, 1465-1473.
PDB code: 2bke
16194225 C.E.Bell (2005).
Structure and mechanism of Escherichia coli RecA ATPase.
  Mol Microbiol, 58, 358-366.  
16148308 C.Neylon, A.V.Kralicek, T.M.Hill, and N.E.Dixon (2005).
Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex.
  Microbiol Mol Biol Rev, 69, 501-526.  
15917244 D.V.Bugreev, E.I.Golub, A.Z.Stasiak, A.Stasiak, and A.V.Mazin (2005).
Activation of human meiosis-specific recombinase Dmc1 by Ca2+.
  J Biol Chem, 280, 26886-26895.  
15902274 G.I.Karras, G.Kustatscher, H.R.Buhecha, M.D.Allen, C.Pugieux, F.Sait, M.Bycroft, and A.G.Ladurner (2005).
The macro domain is an ADP-ribose binding module.
  EMBO J, 24, 1911-1920.
PDB codes: 2bfq 2bfr
15719060 J.M.Cox, O.V.Tsodikov, and M.M.Cox (2005).
Organized unidirectional waves of ATP hydrolysis within a RecA filament.
  PLoS Biol, 3, e52.  
15889153 J.Zaitseva, S.Jenewein, T.Jumpertz, I.B.Holland, and L.Schmitt (2005).
H662 is the linchpin of ATP hydrolysis in the nucleotide-binding domain of the ABC transporter HlyB.
  EMBO J, 24, 1901-1910.
PDB code: 1xef
16051821 T.Xu, A.Sampath, A.Chao, D.Wen, M.Nanao, P.Chene, S.G.Vasudevan, and J.Lescar (2005).
Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A.
  J Virol, 79, 10278-10288.
PDB codes: 2bhr 2bmf
15235592 A.B.Conway, T.W.Lynch, Y.Zhang, G.S.Fortin, C.W.Fung, L.S.Symington, and P.A.Rice (2004).
Crystal structure of a Rad51 filament.
  Nat Struct Mol Biol, 11, 791-796.
PDB code: 1szp
15060027 A.D.Putteet-Driver, J.Zhong, and A.G.Barbour (2004).
Transgenic expression of RecA of the spirochetes Borrelia burgdorferi and Borrelia hermsii in Escherichia coli revealed differences in DNA repair and recombination phenotypes.
  J Bacteriol, 186, 2266-2274.  
15189142 A.L.Davidson, and J.Chen (2004).
ATP-binding cassette transporters in bacteria.
  Annu Rev Biochem, 73, 241-268.  
15359274 D.J.Fitzgerald, C.DeLuca, I.Berger, H.Gaillard, R.Sigrist, K.Schimmele, and T.J.Richmond (2004).
Reaction cycle of the yeast Isw2 chromatin remodeling complex.
  EMBO J, 23, 3836-3843.  
15479787 D.N.Frick, R.S.Rypma, A.M.Lam, and C.M.Frenz (2004).
Electrostatic analysis of the hepatitis C virus NS3 helicase reveals both active and allosteric site locations.
  Nucleic Acids Res, 32, 5519-5528.  
15369673 E.J.Mancini, D.E.Kainov, J.M.Grimes, R.Tuma, D.H.Bamford, and D.I.Stuart (2004).
Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation.
  Cell, 118, 743-755.
PDB codes: 1w44 1w46 1w47 1w48 1w49 1w4a 1w4b 1w4c
15326176 G.Tombline, L.A.Bartholomew, G.A.Tyndall, K.Gimi, I.L.Urbatsch, and A.E.Senior (2004).
Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues.
  J Biol Chem, 279, 46518-46526.  
15585580 H.Shi, O.Cordin, C.M.Minder, P.Linder, and R.M.Xu (2004).
Crystal structure of the human ATP-dependent splicing and export factor UAP56.
  Proc Natl Acad Sci U S A, 101, 17628-17633.
PDB codes: 1xti 1xtj 1xtk
15256595 I.Vakonakis, and A.C.LiWang (2004).
Structure of the C-terminal domain of the clock protein KaiA in complex with a KaiC-derived peptide: implications for KaiC regulation.
  Proc Natl Acad Sci U S A, 101, 10925-10930.
PDB codes: 1suy 1sv1
15310852 J.A.James, A.K.Aggarwal, R.M.Linden, and C.R.Escalante (2004).
Structure of adeno-associated virus type 2 Rep40-ADP complex: insight into nucleotide recognition and catalysis by superfamily 3 helicases.
  Proc Natl Acad Sci U S A, 101, 12455-12460.
PDB code: 1u0j
15466870 J.C.Drees, S.L.Lusetti, and M.M.Cox (2004).
Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state.
  J Biol Chem, 279, 52991-52997.  
14704354 K.A.Miller, D.Sawicka, D.Barsky, and J.S.Albala (2004).
Domain mapping of the Rad51 paralog protein complexes.
  Nucleic Acids Res, 32, 169-178.  
15371437 M.Yoon-Robarts, A.G.Blouin, S.Bleker, J.A.Kleinschmidt, A.K.Aggarwal, C.R.Escalante, and R.M.Linden (2004).
Residues within the B' motif are critical for DNA binding by the superfamily 3 helicase Rep40 of adeno-associated virus type 2.
  J Biol Chem, 279, 50472-50481.  
15128295 N.Tuteja, and R.Tuteja (2004).
Unraveling DNA helicases. Motif, structure, mechanism and function.
  Eur J Biochem, 271, 1849-1863.  
12686593 A.Silvanovich, M.G.Li, M.Serr, S.Mische, and T.S.Hays (2003).
The third P-loop domain in cytoplasmic dynein heavy chain is essential for dynein motor function and ATP-sensitive microtubule binding.
  Mol Biol Cell, 14, 1355-1365.  
12595550 B.Scholz, S.Rechter, J.C.Drach, L.B.Townsend, and E.Bogner (2003).
Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus.
  Nucleic Acids Res, 31, 1426-1433.  
12966089 C.A.French, C.E.Tambini, and J.Thacker (2003).
Identification of functional domains in the RAD51L2 (RAD51C) protein and its requirement for gene conversion.
  J Biol Chem, 278, 45445-45450.  
12941707 D.S.Shin, L.Pellegrini, D.S.Daniels, B.Yelent, L.Craig, D.Bates, D.S.Yu, M.K.Shivji, C.Hitomi, A.S.Arvai, N.Volkmann, H.Tsuruta, T.L.Blundell, A.R.Venkitaraman, and J.A.Tainer (2003).
Full-length archaeal Rad51 structure and mutants: mechanisms for RAD51 assembly and control by BRCA2.
  EMBO J, 22, 4566-4576.
PDB code: 1pzn
14530440 G.Ziegelin, T.Niedenzu, R.Lurz, W.Saenger, and E.Lanka (2003).
Hexameric RSF1010 helicase RepA: the structural and functional importance of single amino acid residues.
  Nucleic Acids Res, 31, 5917-5929.
PDB code: 1olo
14527411 J.Chen, G.Lu, J.Lin, A.L.Davidson, and F.A.Quiocho (2003).
A tweezers-like motion of the ATP-binding cassette dimer in an ABC transport cycle.
  Mol Cell, 12, 651-661.
PDB codes: 1q12 1q1b 1q1e
12480933 M.J.Davey, C.Indiani, and M.O'Donnell (2003).
Reconstitution of the Mcm2-7p heterohexamer, subunit arrangement, and ATP site architecture.
  J Biol Chem, 278, 4491-4499.  
14527291 M.M.Cox (2003).
The bacterial RecA protein as a motor protein.
  Annu Rev Microbiol, 57, 551-577.  
12626715 M.Yoshimasu, H.Aihara, Y.Ito, S.Rajesh, S.Ishibe, T.Mikawa, S.Yokoyama, and T.Shibata (2003).
An NMR study on the interaction of Escherichia coli DinI with RecA-ssDNA complexes.
  Nucleic Acids Res, 31, 1735-1743.  
14610285 R.Perriman, I.Barta, G.K.Voeltz, J.Abelson, and M.Ares (2003).
ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.
  Proc Natl Acad Sci U S A, 100, 13857-13862.  
12808143 S.C.Cordell, E.J.Robinson, and J.Lowe (2003).
Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ.
  Proc Natl Acad Sci U S A, 100, 7889-7894.
PDB codes: 1oft 1ofu
12557189 S.Datta, N.Ganesh, N.R.Chandra, K.Muniyappa, and M.Vijayan (2003).
Structural studies on MtRecA-nucleotide complexes: insights into DNA and nucleotide binding and the structural signature of NTP recognition.
  Proteins, 50, 474-485.
PDB codes: 1mo3 1mo4 1mo5 1mo6
12837805 S.Datta, R.Krishna, N.Ganesh, N.R.Chandra, K.Muniyappa, and M.Vijayan (2003).
Crystal structures of Mycobacterium smegmatis RecA and its nucleotide complexes.
  J Bacteriol, 185, 4280-4284.
PDB codes: 1ubc 1ube 1ubf 1ubg
12598539 S.L.Lusetti, E.A.Wood, C.D.Fleming, M.J.Modica, J.Korth, L.Abbott, D.W.Dwyer, A.I.Roca, R.B.Inman, and M.M.Cox (2003).
C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects.
  J Biol Chem, 278, 16372-16380.  
12595538 S.L.Lusetti, J.J.Shaw, and M.M.Cox (2003).
Magnesium ion-dependent activation of the RecA protein involves the C terminus.
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12727865 S.N.Savvides, H.J.Yeo, M.R.Beck, F.Blaesing, R.Lurz, E.Lanka, R.Buhrdorf, W.Fischer, R.Haas, and G.Waksman (2003).
VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion.
  EMBO J, 22, 1969-1980.
PDB codes: 1nly 1nlz 1opx
11823434 A.C.Rodríguez, and D.Stock (2002).
Crystal structure of reverse gyrase: insights into the positive supercoiling of DNA.
  EMBO J, 21, 418-426.
PDB codes: 1gku 1gl9
11916875 B.C.Butler, R.H.Hanchett, H.Rafailov, and G.MacDonald (2002).
Investigating structural changes induced by nucleotide binding to RecA using difference FTIR.
  Biophys J, 82, 2198-2210.  
12000837 B.C.Godthelp, W.W.Wiegant, A.van Duijn-Goedhart, O.D.Schärer, P.P.van Buul, R.Kanaar, and M.Z.Zdzienicka (2002).
Mammalian Rad51C contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability.
  Nucleic Acids Res, 30, 2172-2182.  
11959500 D.Jeruzalmi, M.O'Donnell, and J.Kuriyan (2002).
Clamp loaders and sliding clamps.
  Curr Opin Struct Biol, 12, 217-224.  
11748238 F.X.Gomis-Rüth, G.Moncalían, F.de la Cruz, and M.Coll (2002).
Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein. Detailed structural features and mapping of the active site cleft.
  J Biol Chem, 277, 7556-7566.  
12065428 G.S.Fortin, and L.S.Symington (2002).
Mutations in yeast Rad51 that partially bypass the requirement for Rad55 and Rad57 in DNA repair by increasing the stability of Rad51-DNA complexes.
  EMBO J, 21, 3160-3170.  
11839741 G.Tombline, C.D.Heinen, K.S.Shim, and R.Fishel (2002).
Biochemical characterization of the human RAD51 protein. III. Modulation of DNA binding by adenosine nucleotides.
  J Biol Chem, 277, 14434-14442.  
11839499 J.M.Caruthers, and D.B.McKay (2002).
Helicase structure and mechanism.
  Curr Opin Struct Biol, 12, 123-133.  
12403627 J.Petersen, K.Fisher, C.J.Mitchell, and D.J.Lowe (2002).
Multiple inequivalent metal-nucleotide coordination environments in the presence of the VO2+-inhibited nitrogenase iron protein: pH-dependent structural rearrangements at the nucleotide binding site.
  Biochemistry, 41, 13253-13263.  
12442171 L.Pellegrini, D.S.Yu, T.Lo, S.Anand, M.Lee, T.L.Blundell, and A.R.Venkitaraman (2002).
Insights into DNA recombination from the structure of a RAD51-BRCA2 complex.
  Nature, 420, 287-293.
PDB code: 1n0w
12415300 M.J.Davey, D.Jeruzalmi, J.Kuriyan, and M.O'Donnell (2002).
Motors and switches: AAA+ machines within the replisome.
  Nat Rev Mol Cell Biol, 3, 826-835.  
11950940 N.S.Frolova, N.Schek, N.Tikhmyanova, and T.R.Coleman (2002).
Xenopus Cdc6 performs separate functions in initiating DNA replication.
  Mol Biol Cell, 13, 1298-1312.  
12235389 P.Soultanas, and D.B.Wigley (2002).
Site-directed mutagenesis reveals roles for conserved amino acid residues in the hexameric DNA helicase DnaB from Bacillus stearothermophilus.
  Nucleic Acids Res, 30, 4051-4060.  
12045091 S.L.Lusetti, and M.M.Cox (2002).
The bacterial RecA protein and the recombinational DNA repair of stalled replication forks.
  Annu Rev Biochem, 71, 71.  
12050150 W.J.Kim, E.J.Park, H.Lee, R.H.Seong, and S.D.Park (2002).
Physical interaction between recombinational proteins Rhp51 and Rad22 in Schizosaccharomyces pombe.
  J Biol Chem, 277, 30264-30270.  
11514535 B.Grabowski, and Z.Kelman (2001).
Autophosphorylation of archaeal Cdc6 homologues is regulated by DNA.
  J Bacteriol, 183, 5459-5464.  
11551790 C.Geourjon, C.Orelle, E.Steinfels, C.Blanchet, G.Deléage, A.Di Pietro, and J.M.Jault (2001).
A common mechanism for ATP hydrolysis in ABC transporter and helicase superfamilies.
  Trends Biochem Sci, 26, 539-544.  
11752455 G.Ebersbach, and K.Gerdes (2001).
The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA.
  Proc Natl Acad Sci U S A, 98, 15078-15083.  
11296216 I.Hayashi, T.Oyama, and K.Morikawa (2001).
Structural and functional studies of MinD ATPase: implications for the molecular recognition of the bacterial cell division apparatus.
  EMBO J, 20, 1819-1828.
PDB codes: 1g3q 1g3r
11337399 J.Christiansen, D.R.Dean, and L.C.Seefeldt (2001).
MECHANISTIC FEATURES OF THE MO-CONTAINING NITROGENASE.
  Annu Rev Plant Physiol Plant Mol Biol, 52, 269-295.  
11342134 J.Kelley De Zutter, A.L.Forget, K.M.Logan, and K.L.Knight (2001).
Phe217 regulates the transfer of allosteric information across the subunit interface of the RecA protein filament.
  Structure, 9, 47-55.  
11251810 K.Karata, C.S.Verma, A.J.Wilkinson, and T.Ogura (2001).
Probing the mechanism of ATP hydrolysis and substrate translocation in the AAA protease FtsH by modelling and mutagenesis.
  Mol Microbiol, 39, 890-903.  
11886553 M.E.Karoui, and J.Errington (2001).
Isolation and characterization of topological specificity mutants of minD in Bacillus subtilis.
  Mol Microbiol, 42, 1211-1221.  
11470432 N.Karpowich, O.Martsinkevich, L.Millen, Y.R.Yuan, P.L.Dai, K.MacVey, P.J.Thomas, and J.F.Hunt (2001).
Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter.
  Structure, 9, 571-586.
PDB codes: 1g6h 1gaj
11171974 R.M.Story, H.Li, and J.N.Abelson (2001).
Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.
  Proc Natl Acad Sci U S A, 98, 1465-1470.
PDB code: 1hv8
11472918 S.Torriani, G.E.Felis, and F.Dellaglio (2001).
Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers.
  Appl Environ Microbiol, 67, 3450-3454.  
10644762 D.A.Sampson, M.E.Arana, and P.E.Boehmer (2000).
Cysteine 111 affects coupling of single-stranded DNA binding to ATP hydrolysis in the herpes simplex virus type-1 origin-binding protein.
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10838059 E.Muneyuki, H.Noji, T.Amano, T.Masaike, and M.Yoshida (2000).
F(0)F(1)-ATP synthase: general structural features of 'ATP-engine' and a problem on free energy transduction.
  Biochim Biophys Acta, 1458, 467-481.  
11163218 H.J.Yeo, S.N.Savvides, A.B.Herr, E.Lanka, and G.Waksman (2000).
Crystal structure of the hexameric traffic ATPase of the Helicobacter pylori type IV secretion system.
  Mol Cell, 6, 1461-1472.
PDB code: 1g6o
11087362 I.L.Urbatsch, M.Julien, I.Carrier, M.E.Rousseau, R.Cayrol, and P.Gros (2000).
Mutational analysis of conserved carboxylate residues in the nucleotide binding sites of P-glycoprotein.
  Biochemistry, 39, 14138-14149.  
10713991 K.A.Denessiouk, and M.S.Johnson (2000).
When fold is not important: a common structural framework for adenine and AMP binding in 12 unrelated protein families.
  Proteins, 38, 310-326.  
10892646 M.R.Singleton, M.R.Sawaya, T.Ellenberger, and D.B.Wigley (2000).
Crystal structure of T7 gene 4 ring helicase indicates a mechanism for sequential hydrolysis of nucleotides.
  Cell, 101, 589-600.
PDB codes: 1e0j 1e0k
11044135 M.S.Marín, R.Casais, J.M.Alonso, and F.Parra (2000).
ATP binding and ATPase activities associated with recombinant rabbit hemorrhagic disease virus 2C-like polypeptide.
  J Virol, 74, 10846-10851.  
11304669 N.Sugimoto (2000).
DNA recognition of a 24-mer peptide derived from RecA protein.
  Biopolymers, 55, 416-424.  
10766770 P.Askjaer, R.Rosendahl, and J.Kjems (2000).
Nuclear export of the DEAD box An3 protein by CRM1 is coupled to An3 helicase activity.
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11101289 S.B.Jang, L.C.Seefeldt, and J.W.Peters (2000).
Insights into nucleotide signal transduction in nitrogenase: structure of an iron protein with MgADP bound.
  Biochemistry, 39, 14745-14752.
PDB code: 1fp6
11121488 S.Datta, M.M.Prabu, M.B.Vaze, N.Ganesh, N.R.Chandra, K.Muniyappa, and M.Vijayan (2000).
Crystal structures of Mycobacterium tuberculosis RecA and its complex with ADP-AlF(4): implications for decreased ATPase activity and molecular aggregation.
  Nucleic Acids Res, 28, 4964-4973.
PDB codes: 1g18 1g19
10966472 S.S.Patel, and K.M.Picha (2000).
Structure and function of hexameric helicases.
  Annu Rev Biochem, 69, 651-697.  
10966477 T.J.Kelly, and G.W.Brown (2000).
Regulation of chromosome replication.
  Annu Rev Biochem, 69, 829-880.  
  9882335 A.Martins, C.H.Gross, and S.Shuman (1999).
Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase I, a DNA-dependent ATPase of the DExH box family.
  J Virol, 73, 1302-1308.  
  10490626 C.Morrison, A.Shinohara, E.Sonoda, Y.Yamaguchi-Iwai, M.Takata, R.R.Weichselbaum, and S.Takeda (1999).
The essential functions of human Rad51 are independent of ATP hydrolysis.
  Mol Cell Biol, 19, 6891-6897.  
10521428 C.Y.Hu, W.Chen, and W.D.Frasch (1999).
Metal ligation by Walker homology B aspartate betaD262 at site 3 of the latent but not activated form of the chloroplast F(1)-ATPase from Chlamydomonas reinhardtii.
  J Biol Chem, 274, 30481-30486.  
  10101168 D.DeRyckere, C.L.Smith, and G.S.Martin (1999).
The role of nucleotide binding and hydrolysis in the function of the fission yeast cdc18(+) gene product.
  Genetics, 151, 1445-1457.  
10540281 E.N.Zaitsev, and S.C.Kowalczykowski (1999).
Enhanced monomer-monomer interactions can suppress the recombination deficiency of the recA142 allele.
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10606264 E.R.Johnson, and D.B.McKay (1999).
Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase.
  RNA, 5, 1526-1534.
PDB code: 1qva
10089426 G.Schmees, K.Höner zu Bentrup, E.Schneider, D.Vinzenz, and U.Ermler (1999).
Crystallization and preliminary X-ray analysis of the bacterial ATP-binding-cassette (ABC) protein MalK.
  Acta Crystallogr D Biol Crystallogr, 55, 285-286.  
10512836 H.Yagi, K.Tozawa, N.Sekino, T.Iwabuchi, M.Yoshida, and H.Akutsu (1999).
Functional conformation changes in the TF(1)-ATPase beta subunit probed by 12 tyrosine residues.
  Biophys J, 77, 2175-2183.  
  10419963 I.Rombel, P.Peters-Wendisch, A.Mesecar, T.Thorgeirsson, Y.K.Shin, and S.Kustu (1999).
MgATP binding and hydrolysis determinants of NtrC, a bacterial enhancer-binding protein.
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10619014 J.D.Quisel, D.C.Lin, and A.D.Grossman (1999).
Control of development by altered localization of a transcription factor in B. subtilis.
  Mol Cell, 4, 665-672.  
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Mutations affecting motifs of unknown function in the central domain of nitrogen regulatory protein C.
  J Bacteriol, 181, 5443-5454.  
10022846 K.Nishiyama, A.Fukuda, K.Morita, and H.Tokuda (1999).
Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation.
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10594814 M.C.Hall, and S.W.Matson (1999).
Helicase motifs: the engine that powers DNA unwinding.
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10468570 M.Hegner, S.B.Smith, and C.Bustamante (1999).
Polymerization and mechanical properties of single RecA-DNA filaments.
  Proc Natl Acad Sci U S A, 96, 10109-10114.  
10535735 M.R.Sawaya, S.Guo, S.Tabor, C.C.Richardson, and T.Ellenberger (1999).
Crystal structure of the helicase domain from the replicative helicase-primase of bacteriophage T7.
  Cell, 99, 167-177.
PDB codes: 1cr0 1cr1 1cr2 1cr4
9892652 M.Weinreich, C.Liang, and B.Stillman (1999).
The Cdc6p nucleotide-binding motif is required for loading mcm proteins onto chromatin.
  Proc Natl Acad Sci U S A, 96, 441-446.  
10464326 P.Kaur (1999).
The anion-stimulated ATPase ArsA shows unisite and multisite catalytic activity.
  J Biol Chem, 274, 25849-25854.  
10361278 R.Ebbert, A.Birkmann, and H.J.Schüller (1999).
The product of the SNF2/SWI2 paralogue INO80 of Saccharomyces cerevisiae required for efficient expression of various yeast structural genes is part of a high-molecular-weight protein complex.
  Mol Microbiol, 32, 741-751.  
10092842 R.Kato, and S.Kuramitsu (1999).
Characterization of thermostable RecA protein and analysis of its interaction with single-stranded DNA.
  Eur J Biochem, 259, 592-601.  
  9922255 S.J.Sandler, P.Hugenholtz, C.Schleper, E.F.DeLong, N.R.Pace, and A.J.Clark (1999).
Diversity of radA genes from cultured and uncultured archaea: comparative analysis of putative RadA proteins and their use as a phylogenetic marker.
  J Bacteriol, 181, 907-915.  
10464259 T.Hishida, H.Iwasaki, T.Yagi, and H.Shinagawa (1999).
Role of walker motif A of RuvB protein in promoting branch migration of holliday junctions. Walker motif a mutations affect Atp binding, Atp hydrolyzing, and DNA binding activities of Ruvb.
  J Biol Chem, 274, 25335-25342.  
10318792 T.Zhou, and B.P.Rosen (1999).
Asp45 is a Mg2+ ligand in the ArsA ATPase.
  J Biol Chem, 274, 13854-13858.  
  10436018 U.Herbig, C.A.Marlar, and E.Fanning (1999).
The Cdc6 nucleotide-binding site regulates its activity in DNA replication in human cells.
  Mol Biol Cell, 10, 2631-2645.  
10066766 W.Chen, R.LoBrutto, and W.D.Frasch (1999).
EPR spectroscopy of VO2+-ATP bound to catalytic site 3 of chloroplast F1-ATPase from Chlamydomonas reveals changes in metal ligation resulting from mutations to the phosphate-binding loop threonine (betaT168).
  J Biol Chem, 274, 7089-7094.  
9601030 B.D.Grant, W.Hemmer, I.Tsigelny, J.A.Adams, and S.S.Taylor (1998).
Kinetic analyses of mutations in the glycine-rich loop of cAMP-dependent protein kinase.
  Biochemistry, 37, 7708-7715.  
  9573237 C.H.Gross, and S.Shuman (1998).
The nucleoside triphosphatase and helicase activities of vaccinia virus NPH-II are essential for virus replication.
  J Virol, 72, 4729-4736.  
9642255 E.H.Bursey, and B.K.Burgess (1998).
Characterization of a variant iron protein of nitrogenase that is impaired in its ability to adopt the MgATP-induced conformational change.
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9525874 E.L.Hildebrand, and L.Grossman (1998).
Introduction of a tryptophan reporter group into the ATP binding motif of the Escherichia coli UvrB protein for the study of nucleotide binding and conformational dynamics.
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9640644 E.Schneider, and S.Hunke (1998).
ATP-binding-cassette (ABC) transport systems: functional and structural aspects of the ATP-hydrolyzing subunits/domains.
  FEMS Microbiol Rev, 22, 1.  
9704406 F.J.Kull, and R.J.Fletterick (1998).
Is the tubulin/FtsZ fold related to the G-protein fold?
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9521782 F.J.van de Loo, and M.E.Salvucci (1998).
Involvement of two aspartate residues of Rubisco activase in coordination of the ATP gamma-phosphate and subunit cooperativity.
  Biochemistry, 37, 4621-4625.  
9702188 G.Perkins, and J.F.Diffley (1998).
Nucleotide-dependent prereplicative complex assembly by Cdc6p, a homolog of eukaryotic and prokaryotic clamp-loaders.
  Mol Cell, 2, 23-32.  
  9611193 H.R.Hotz, and B.Schwer (1998).
Mutational analysis of the yeast DEAH-box splicing factor Prp16.
  Genetics, 149, 807-815.  
9614113 H.S.Cho, N.C.Ha, L.W.Kang, K.M.Chung, S.H.Back, S.K.Jang, and B.H.Oh (1998).
Crystal structure of RNA helicase from genotype 1b hepatitis C virus. A feasible mechanism of unwinding duplex RNA.
  J Biol Chem, 273, 15045-15052.
PDB code: 8ohm
9843365 I.I.Mathews, M.D.Erion, and S.E.Ealick (1998).
Structure of human adenosine kinase at 1.5 A resolution.
  Biochemistry, 37, 15607-15620.
PDB code: 1bx4
10384279 I.Rombel, A.North, I.Hwang, C.Wyman, and S.Kustu (1998).
The bacterial enhancer-binding protein NtrC as a molecular machine.
  Cold Spring Harb Symp Quant Biol, 63, 157-166.  
9751713 J.Cai, N.Yao, E.Gibbs, J.Finkelstein, B.Phillips, M.O'Donnell, and J.Hurwitz (1998).
ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits.
  Proc Natl Acad Sci U S A, 95, 11607-11612.  
9506981 J.Li, and B.P.Rosen (1998).
Steric limitations in the interaction of the ATP binding domains of the ArsA ATPase.
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9485365 J.R.Lorsch, and D.Herschlag (1998).
The DEAD box protein eIF4A. 2. A cycle of nucleotide and RNA-dependent conformational changes.
  Biochemistry, 37, 2194-2206.  
9519291 L.E.Bird, H.S.Subramanya, and D.B.Wigley (1998).
Helicases: a unifying structural theme?
  Curr Opin Struct Biol, 8, 14-18.  
9736628 M.Yamauchi, and T.A.Baker (1998).
An ATP-ADP switch in MuB controls progression of the Mu transposition pathway.
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9724525 R.Masui, and S.Kuramitsu (1998).
Probing of DNA-binding sites of Escherichia coli RecA protein utilizing 1-anilinonaphthalene-8-sulfonic acid.
  Biochemistry, 37, 12133-12143.  
  9696748 W.A.Klimke, and L.S.Frost (1998).
Genetic analysis of the role of the transfer gene, traN, of the F and R100-1 plasmids in mating pair stabilization during conjugation.
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9305945 A.Bouhss, D.Mengin-Lecreulx, D.Blanot, J.van Heijenoort, and C.Parquet (1997).
Invariant amino acids in the Mur peptide synthetases of bacterial peptidoglycan synthesis and their modification by site-directed mutagenesis in the UDP-MurNAc:L-alanine ligase from Escherichia coli.
  Biochemistry, 36, 11556-11563.  
9171366 B.Clever, H.Interthal, J.Schmuckli-Maurer, J.King, M.Sigrist, and W.D.Heyer (1997).
Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins.
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9363942 B.Guenther, R.Onrust, A.Sali, M.O'Donnell, and J.Kuriyan (1997).
Crystal structure of the delta' subunit of the clamp-loader complex of E. coli DNA polymerase III.
  Cell, 91, 335-345.
PDB code: 1a5t
  9371600 D.W.Kim, J.Kim, Y.Gwack, J.H.Han, and J.Choe (1997).
Mutational analysis of the hepatitis C virus RNA helicase.
  J Virol, 71, 9400-9409.  
9325305 J.A.Kelley, and K.L.Knight (1997).
Allosteric regulation of RecA protein function is mediated by Gln194.
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9020191 K.L.Graves-Woodward, J.Gottlieb, M.D.Challberg, and S.K.Weller (1997).
Biochemical analyses of mutations in the HSV-1 helicase-primase that alter ATP hydrolysis, DNA unwinding, and coupling between hydrolysis and unwinding.
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9185573 L.E.Bird, K.Hâkansson, H.Pan, and D.B.Wigley (1997).
Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein.
  Nucleic Acids Res, 25, 2620-2626.  
9038340 R.D.Klemm, R.J.Austin, and S.P.Bell (1997).
Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex.
  Cell, 88, 493-502.  
9346912 R.Masui, T.Mikawa, and S.Kuramitsu (1997).
Local folding of the N-terminal domain of Escherichia coli RecA controls protein-protein interaction.
  J Biol Chem, 272, 27707-27715.  
  9032352 S.Noble, and M.L.Nibert (1997).
Characterization of an ATPase activity in reovirus cores and its genetic association with core-shell protein lambda1.
  J Virol, 71, 2182-2191.  
9396812 T.H.Chang, L.J.Latus, Z.Liu, and J.M.Abbott (1997).
Genetic interactions of conserved regions in the DEAD-box protein Prp28p.
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  8550524 A.Marais, J.M.Bové, and J.Renaudin (1996).
Spiroplasma citri virus SpV1-derived cloning vector: deletion formation by illegitimate and homologous recombination in a spiroplasmal host strain which probably lacks a functional recA gene.
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8785318 C.A.Smith, and I.Rayment (1996).
Active site comparisons highlight structural similarities between myosin and other P-loop proteins.
  Biophys J, 70, 1590-1602.  
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CFTR: the nucleotide binding folds regulate the accessibility and stability of the activated state.
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8663445 D.Vertommen, L.Bertrand, B.Sontag, A.Di Pietro, M.P.Louckx, H.Vidal, L.Hue, and M.H.Rider (1996).
The ATP-binding site in the 2-kinase domain of liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Study of the role of Lys-54 and Thr-55 by site-directed mutagenesis.
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  8943355 E.J.Steinmetz, and D.A.Brow (1996).
Repression of gene expression by an exogenous sequence element acting in concert with a heterogeneous nuclear ribonucleoprotein-like protein, Nrd1, and the putative helicase Sen1.
  Mol Cell Biol, 16, 6993-7003.  
8606779 F.J.Kull, E.P.Sablin, R.Lau, R.J.Fletterick, and R.D.Vale (1996).
Crystal structure of the kinesin motor domain reveals a structural similarity to myosin.
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PDB code: 1bg2
8951093 H.Noji, T.Amano, and M.Yoshida (1996).
Molecular switch of F0F1-ATP synthase, G-protein, and other ATP-driven enzymes.
  J Bioenerg Biomembr, 28, 451-457.  
8662632 H.Shen, A.Sosa-Peinado, and D.M.Mueller (1996).
Intragenic suppressors of P-loop mutations in the beta-subunit of the mitochondrial ATPase in the yeast Saccharomyces cerevisiae.
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The alpha3beta3gamma subcomplex of the F1-ATPase from the thermophilic bacillus PS3 with the betaT165S substitution does not entrap inhibitory MgADP in a catalytic site during turnover.
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8916905 J.M.de Pereda, D.Leynadier, J.A.Evangelio, P.Chacón, and J.M.Andreu (1996).
Tubulin secondary structure analysis, limited proteolysis sites, and homology to FtsZ.
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8702501 J.Marszalek, W.Zhang, T.R.Hupp, C.Margulies, K.M.Carr, S.Cherry, and J.M.Kaguni (1996).
Domains of DnaA protein involved in interaction with DnaB protein, and in unwinding the Escherichia coli chromosomal origin.
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8702526 J.Weber, C.Bowman, and A.E.Senior (1996).
Specific tryptophan substitution in catalytic sites of Escherichia coli F1-ATPase allows differentiation between bound substrate ATP and product ADP in steady-state catalysis.
  J Biol Chem, 271, 18711-18718.  
8809781 K.M.Carr, and J.M.Kaguni (1996).
The A184V missense mutation of the dnaA5 and dnaA46 alleles confers a defect in ATP binding and thermolability in initiation of Escherichia coli DNA replication.
  Mol Microbiol, 20, 1307-1318.  
  8797840 K.Morimatsu, F.Maraboeuf, P.Hagmar, B.Nordén, T.Horii, and M.Takahashi (1996).
Roles of Tyr103 and Tyr264 in the regulation of RecA-DNA interactions by nucleotide cofactors.
  Eur J Biochem, 240, 91-97.  
8663354 K.Sato, H.Mori, M.Yoshida, and S.Mizushima (1996).
Characterization of a potential catalytic residue, Asp-133, in the high affinity ATP-binding site of Escherichia coli SecA, translocation ATPase.
  J Biol Chem, 271, 17439-17444.  
8599762 L.W.Tari, A.Matte, U.Pugazhenthi, H.Goldie, and L.T.Delbaere (1996).
Snapshot of an enzyme reaction intermediate in the structure of the ATP-Mg2+-oxalate ternary complex of Escherichia coli PEP carboxykinase.
  Nat Struct Biol, 3, 355-363.
PDB code: 1ayl
8900164 M.T.Washington, A.H.Rosenberg, K.Griffin, F.W.Studier, and S.S.Patel (1996).
Biochemical analysis of mutant T7 primase/helicase proteins defective in DNA binding, nucleotide hydrolysis, and the coupling of hydrolysis with DNA unwinding.
  J Biol Chem, 271, 26825-26834.  
8954148 M.Takahashi, F.Maraboeuf, and B.Nordén (1996).
Locations of functional domains in the RecA protein. Overlap of domains and regulation of activities.
  Eur J Biochem, 242, 20-28.  
8639660 R.A.Kumar, M.B.Vaze, N.R.Chandra, M.Vijayan, and K.Muniyappa (1996).
Functional characterization of the precursor and spliced forms of RecA protein of Mycobacterium tuberculosis.
  Biochemistry, 35, 1793-1802.  
  8756636 R.Chanet, M.Heude, A.Adjiri, L.Maloisel, and F.Fabre (1996).
Semidominant mutations in the yeast Rad51 protein and their relationships with the Srs2 helicase.
  Mol Cell Biol, 16, 4782-4789.  
8896589 R.D.Vale (1996).
Switches, latches, and amplifiers: common themes of G proteins and molecular motors.
  J Cell Biol, 135, 291-302.  
8612068 R.Sakowicz, and L.S.Goldstein (1996).
The muscle in kinesin.
  Nat Struct Biol, 3, 404-407.  
8668545 S.J.Sandler, L.H.Satin, H.S.Samra, and A.J.Clark (1996).
recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae.
  Nucleic Acids Res, 24, 2125-2132.  
  8606161 S.Karlin, and L.Brocchieri (1996).
Evolutionary conservation of RecA genes in relation to protein structure and function.
  J Bacteriol, 178, 1881-1894.  
8871566 S.Pietrokovski (1996).
Searching databases of conserved sequence regions by aligning protein multiple-alignments.
  Nucleic Acids Res, 24, 3836-3845.  
8662640 W.M.Rehrauer, and S.C.Kowalczykowski (1996).
The DNA binding site(s) of the Escherichia coli RecA protein.
  J Biol Chem, 271, 11996-12002.  
8639507 Y.Wang, and K.Adzuma (1996).
Differential proximity probing of two DNA binding sites in the Escherichia coli recA protein using photo-cross-linking methods.
  Biochemistry, 35, 3563-3571.  
7777563 C.Desbiez, C.David, A.Mettouchi, J.Laufs, and B.Gronenborn (1995).
Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication.
  Proc Natl Acad Sci U S A, 92, 5640-5644.  
7588804 C.Ellouze, M.Takahashi, P.Wittung, K.Mortensen, M.Schnarr, and B.Nordén (1995).
Evidence for elongation of the helical pitch of the RecA filament upon ATP and ADP binding using small-angle neutron scattering.
  Eur J Biochem, 233, 579-583.  
  7609038 C.H.Gross, and S.Shuman (1995).
Mutational analysis of vaccinia virus nucleoside triphosphate phosphohydrolase II, a DExH box RNA helicase.
  J Virol, 69, 4727-4736.  
7642557 J.P.van der Wolk, M.Klose, J.G.de Wit, T.den Blaauwen, R.Freudl, and A.J.Driessen (1995).
Identification of the magnesium-binding domain of the high-affinity ATP-binding site of the Bacillus subtilis and Escherichia coli SecA protein.
  J Biol Chem, 270, 18975-18982.  
7737177 K.Morimatsu, T.Horii, and M.Takahashi (1995).
Interaction of Tyr103 and Tyr264 of the RecA protein with DNA and nucleotide cofactors. Fluorescence study of engineered proteins.
  Eur J Biochem, 228, 779-785.  
8837896 M.Rémond, P.Sheldrick, F.Lebreton, and T.Foulon (1995).
Sequence of the canine herpesvirus thymidine kinase gene: taxon-preferred amino acid residues in the alphaherpesviral thymidine kinases.
  Virus Res, 39, 341-354.  
7882996 P.Wittung, B.Nordén, and M.Takahashi (1995).
Secondary structure of RecA in solution. The effects of cofactor, DNA and ionic conditions.
  Eur J Biochem, 228, 149-154.  
7588783 R.V.Gardner, O.N.Voloshin, and R.D.Camerini-Otero (1995).
The identification of the single-stranded DNA-binding domain of the Escherichia coli RecA protein.
  Eur J Biochem, 233, 419-425.  
  7853501 S.F.Cotmore, J.Christensen, J.P.Nüesch, and P.Tattersall (1995).
The NS1 polypeptide of the murine parvovirus minute virus of mice binds to DNA sequences containing the motif [ACCA]2-3.
  J Virol, 69, 1652-1660.  
7662362 T.Hirano, T.J.Mitchison, and J.R.Swedlow (1995).
The SMC family: from chromosome condensation to dosage compensation.
  Curr Opin Cell Biol, 7, 329-336.  
8530434 V.A.Malkov, and R.D.Camerini-Otero (1995).
Photocross-links between single-stranded DNA and Escherichia coli RecA protein map to loops L1 (amino acid residues 157-164) and L2 (amino acid residues 195-209).
  J Biol Chem, 270, 30230-30233.  
8048162 D.Bossemeyer (1994).
The glycine-rich sequence of protein kinases: a multifunctional element.
  Trends Biochem Sci, 19, 201-205.  
8026510 D.M.Mueller, V.Indyk, and L.McGill (1994).
ATPase kinetics for wild-type Saccharomyces cerevisiae F1-ATPase and F1-ATPase with the beta-subunit Thr197-->Ser mutation.
  Eur J Biochem, 222, 991-999.  
  8113181 E.Angov, and R.D.Camerini-Otero (1994).
The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization.
  J Bacteriol, 176, 1405-1412.  
  7988572 F.E.Benson, A.Stasiak, and S.C.West (1994).
Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA.
  EMBO J, 13, 5764-5771.  
  8070396 J.Czworkowski, J.Wang, T.A.Steitz, and P.B.Moore (1994).
The crystal structure of elongation factor G complexed with GDP, at 2.7 A resolution.
  EMBO J, 13, 3661-3668.
PDB codes: 1efg 2efg
  8289794 J.H.Lammers, H.H.Offenberg, M.van Aalderen, A.C.Vink, A.J.Dietrich, and C.Heyting (1994).
The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte-regulated genes.
  Mol Cell Biol, 14, 1137-1146.  
7934905 L.A.Gregg-Jolly, and L.N.Ornston (1994).
Properties of Acinetobacter calcoaceticus recA and its contribution to intracellular gene conversion.
  Mol Microbiol, 12, 985-992.  
  8196650 L.Ma, A.Westbroek, A.G.Jochemsen, G.Weeda, A.Bosch, D.Bootsma, J.H.Hoeijmakers, and A.J.van der Eb (1994).
Mutational analysis of ERCC3, which is involved in DNA repair and transcription initiation: identification of domains essential for the DNA repair function.
  Mol Cell Biol, 14, 4126-4134.  
7664057 M.B.Swindells (1994).
Loopy similarities.
  Nat Struct Biol, 1, 421-422.  
7957225 M.G.Duyvis, H.Wassink, and H.Haaker (1994).
Pre-steady-state MgATP-dependent proton production and electron transfer by nitrogenase from Azotobacter vinelandii.
  Eur J Biochem, 225, 881-890.  
7932861 M.Gravel, D.DeAngelis, and P.E.Braun (1994).
Molecular cloning and characterization of rat brain 2',3'-cyclic nucleotide 3'-phosphodiesterase isoform 2.
  J Neurosci Res, 38, 243-247.  
8048163 M.M.Cox (1994).
Why does RecA protein hydrolyse ATP?
  Trends Biochem Sci, 19, 217-222.  
8057853 O.Possot, and A.P.Pugsley (1994).
Molecular characterization of PulE, a protein required for pullulanase secretion.
  Mol Microbiol, 12, 287-299.  
7815090 P.Kern, R.M.Brunne, and G.Folkers (1994).
Nucleotide-binding properties of adenylate kinase from Escherichia coli: a molecular dynamics study in aqueous and vacuum environments.
  J Comput Aided Mol Des, 8, 367-388.  
  7968921 S.C.Kowalczykowski, D.A.Dixon, A.K.Eggleston, S.D.Lauder, and W.M.Rehrauer (1994).
Biochemistry of homologous recombination in Escherichia coli.
  Microbiol Rev, 58, 401-465.  
  8150281 S.Clark-Maguire, and P.E.Mains (1994).
mei-1, a gene required for meiotic spindle formation in Caenorhabditis elegans, is a member of a family of ATPases.
  Genetics, 136, 533-546.  
  8132449 S.Woestyn, A.Allaoui, P.Wattiau, and G.R.Cornelis (1994).
YscN, the putative energizer of the Yersinia Yop secretion machinery.
  J Bacteriol, 176, 1561-1569.  
8200357 T.W.Traut (1994).
The functions and consensus motifs of nine types of peptide segments that form different types of nucleotide-binding sites.
  Eur J Biochem, 222, 9.  
8143796 W.D.Heyer (1994).
The search for the right partner: homologous pairing and DNA strand exchange proteins in eukaryotes.
  Experientia, 50, 223-233.  
  8413273 A.Pause, N.Méthot, and N.Sonenberg (1993).
The HRIGRXXR region of the DEAD box RNA helicase eukaryotic translation initiation factor 4A is required for RNA binding and ATP hydrolysis.
  Mol Cell Biol, 13, 6789-6798.  
8355662 C.Cazaux, A.M.Mazard, and M.Defais (1993).
Inducibility of the SOS response in a recA730 or recA441 strain is restored by transformation with a new recA allele.
  Mol Gen Genet, 240, 296-301.  
7968527 C.Mitchell, and D.Oliver (1993).
Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase.
  Mol Microbiol, 10, 483-497.  
8279127 E.V.Koonin, and T.G.Senkevich (1993).
Fowlpox virus encodes a protein related to human deoxycytidine kinase: further evidence for independent acquisition of genes for enzymes of nucleotide metabolism by different viruses.
  Virus Genes, 7, 289-295.  
7763979 J.A.Tainer, and R.P.Cunningham (1993).
Molecular recognition in DNA-binding proteins and enzymes.
  Curr Opin Biotechnol, 4, 474-483.  
8497195 J.van der Wolk, M.Klose, E.Breukink, R.A.Demel, B.de Kruijff, R.Freudl, and A.J.Driessen (1993).
Characterization of a Bacillus subtilis SecA mutant protein deficient in translocation ATPase and release from the membrane.
  Mol Microbiol, 8, 31-42.  
  8298462 M.B.Swindells (1993).
Classification of doubly wound nucleotide binding topologies using automated loop searches.
  Protein Sci, 2, 2146-2153.  
  8454201 M.Heude, and F.Fabre (1993).
a/alpha-control of DNA repair in the yeast Saccharomyces cerevisiae: genetic and physiological aspects.
  Genetics, 133, 489-498.  
  8407828 S.K.Liu, J.A.Eisen, P.C.Hanawalt, and I.Tessman (1993).
recA mutations that reduce the constitutive coprotease activity of the RecA1202(Prtc) protein: possible involvement of interfilament association in proteolytic and recombination activities.
  J Bacteriol, 175, 6518-6529.  
7934872 S.Sommer, A.Bailone, and R.Devoret (1993).
The appearance of the UmuD'C protein complex in Escherichia coli switches repair from homologous recombination to SOS mutagenesis.
  Mol Microbiol, 10, 963-971.  
  8344265 T.Asai, S.Sommer, A.Bailone, and T.Kogoma (1993).
Homologous recombination-dependent initiation of DNA replication from DNA damage-inducible origins in Escherichia coli.
  EMBO J, 12, 3287-3295.  
  1620127 A.Aboussekhra, R.Chanet, A.Adjiri, and F.Fabre (1992).
Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins.
  Mol Cell Biol, 12, 3224-3234.  
  1378397 A.Pause, and N.Sonenberg (1992).
Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A.
  EMBO J, 11, 2643-2654.  
1581961 A.Shinohara, H.Ogawa, and T.Ogawa (1992).
Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein.
  Cell, 69, 457-470.  
1581960 D.K.Bishop, D.Park, L.Xu, and N.Kleckner (1992).
DMC1: a meiosis-specific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression.
  Cell, 69, 439-456.  
  1400177 F.Larminat, C.Cazaux, M.Germanier, and M.Defais (1992).
New mutations in and around the L2 disordered loop of the RecA protein modulate recombination and/or coprotease activity.
  J Bacteriol, 174, 6264-6269.  
  1514816 P.Duwat, S.D.Ehrlich, and A.Gruss (1992).
Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp. lactis recA gene.
  Appl Environ Microbiol, 58, 2674-2678.  
  1629178 P.Duwat, S.D.Ehrlich, and A.Gruss (1992).
A general method for cloning recA genes of gram-positive bacteria by polymerase chain reaction.
  J Bacteriol, 174, 5171-5175.  
1454527 S.Hsieh, and D.A.Julin (1992).
Alteration by site-directed mutagenesis of the conserved lysine residue in the consensus ATP-binding sequence of the RecB protein of Escherichia coli.
  Nucleic Acids Res, 20, 5647-5653.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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