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UniProt functional annotation for O28126 | ||
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| UniProt code: O28126. |
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| Organism: | |
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126). |
| Taxonomy: | |
Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus. |
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| Function: | |
Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. {ECO:0000269|PubMed:14592988}. |
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| Catalytic activity: | |
Reaction=a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate; Xref=Rhea:RHEA:14433, Rhea:RHEA-COMP:10465, Rhea:RHEA- COMP:10468, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:74896, ChEBI:CHEBI:83071; EC=2.7.7.72; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:14592988, ECO:0000269|PubMed:14636575}. |
| Miscellaneous: | |
A single active site specifically recognizes both ATP and CTP and is responsible for their addition. In contrast to the eubacterial homologs, the specificity of the nucleotide-binding pocket is determined by both the enzyme and the tRNA. |
| Similarity: | |
Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.