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PDBsum entry 1r5u
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Transcription
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PDB id
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1r5u
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Contents |
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1380 a.a.
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1097 a.a.
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266 a.a.
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214 a.a.
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84 a.a.
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133 a.a.
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118 a.a.
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65 a.a.
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114 a.a.
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46 a.a.
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86 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of transcription: an RNA polymerase ii-Tfiib cocrystal at 4.5 angstroms.
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Authors
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D.A.Bushnell,
K.D.Westover,
R.E.Davis,
R.D.Kornberg.
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Ref.
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Science, 2004,
303,
983-988.
[DOI no: ]
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PubMed id
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Abstract
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The structure of the general transcription factor IIB (TFIIB) in a complex with
RNA polymerase II reveals three features crucial for transcription initiation:
an N-terminal zinc ribbon domain of TFIIB that contacts the "dock"
domain of the polymerase, near the path of RNA exit from a transcribing enzyme;
a "finger" domain of TFIIB that is inserted into the polymerase active
center; and a C-terminal domain, whose interaction with both the polymerase and
with a TATA box-binding protein (TBP)-promoter DNA complex orients the DNA for
unwinding and transcription. TFIIB stabilizes an early initiation complex,
containing an incomplete RNA-DNA hybrid region. It may interact with the
template strand, which sets the location of the transcription start site, and
may interfere with RNA exit, which leads to abortive initiation or promoter
escape. The trajectory of promoter DNA determined by the C-terminal domain of
TFIIB traverses sites of interaction with TFIIE, TFIIF, and TFIIH, serving to
define their roles in the transcription initiation process.
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Figure 4.
Fig. 4. Interaction of the B finger domain of TFIIB[N] with DNA
template and RNA transcript. A stereo pair is shown, including
the B finger domain from the pol II-TFIIB[N] structure, RNA-DNA
hybrid helix from the pol II transcribing complex structure (5),
and active site Mg ion. Color code is shown below.
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Figure 5.
Fig. 5. Stabilization of a transcription initiation complex,
containing a short transcript, by TFIIB. (A) A single strand of
DNA was bound to the surface of a Biacore Biosensor chip.
Combinations of pol II, TFIIB, and a five-residue RNA
complementary to the DNA were applied as indicated, and the
change in refractive index near the chip surface, in resonance
units (ru), was measured as a function of time. (B) Same as (A)
but with nine-residue RNA.
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The above figures are
reprinted
by permission from the AAAs:
Science
(2004,
303,
983-988)
copyright 2004.
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