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PDBsum entry 1r4o
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Transcription/DNA
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PDB id
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1r4o
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Crystallographic analysis of the interaction of the glucocorticoid receptor with DNA.
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Authors
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B.F.Luisi,
W.X.Xu,
Z.Otwinowski,
L.P.Freedman,
K.R.Yamamoto,
P.B.Sigler.
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Ref.
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Nature, 1991,
352,
497-505.
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PubMed id
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Abstract
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Two crystal structures of the glucocorticoid receptor DNA-binding domain
complexed with DNA are reported. The domain has a globular fold which contains
two Zn-nucleated substructures of distinct conformation and function. When it
binds DNA, the domain dimerizes, placing the subunits in adjacent major grooves.
In one complex, the DNA has the symmetrical consensus target sequence; in the
second, the central spacing between the target's half-sites is larger by one
base pair. This results in one subunit interacting specifically with the
consensus target half-site and the other nonspecifically with a noncognate
element. The DNA-induced dimer fixes the separation of the subunits' recognition
surfaces so that the spacing between the half-sites becomes a critical feature
of the target sequence's identity.
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Secondary reference #1
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Title
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The basis for half-Site specificity explored through a non-Cognate steroid receptor-Dna complex.
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Authors
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D.T.Gewirth,
P.B.Sigler.
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Ref.
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Nat Struct Biol, 1995,
2,
386-394.
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PubMed id
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