UniProt functional annotation for P84131

UniProt code: P84131.

Organism: Geobacillus stearothermophilus (Bacillus stearothermophilus).
Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
 
Function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. {ECO:0000256|HAMAP-Rule:MF_00103, ECO:0000256|SAAS:SAAS00020861}.
 
Catalytic activity: Hydrolysis of DNA containing ring-opened 7- methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N- methyl)formamidopyrimidine. {ECO:0000256|HAMAP-Rule:MF_00103, ECO:0000256|SAAS:SAAS00020866}.
Catalytic activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. {ECO:0000256|HAMAP-Rule:MF_00103, ECO:0000256|SAAS:SAAS00054021}.
Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP- Rule:MF_00103, ECO:0000256|SAAS:SAAS00169713}; Note=Binds 1 zinc ion per subunit. {ECO:0000256|HAMAP- Rule:MF_00103, ECO:0000256|SAAS:SAAS00169713};
Subunit: Monomer. {ECO:0000256|HAMAP-Rule:MF_00103, ECO:0000256|SAAS:SAAS00020849}.
Similarity: Belongs to the FPG family. {ECO:0000256|HAMAP- Rule:MF_00103}.
Similarity: Contains 1 FPG-type zinc finger. {ECO:0000256|HAMAP- Rule:MF_00103}.

Annotations taken from UniProtKB at the EBI.