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PDBsum entry 1qz7
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protein Protein-protein interface(s) links
Cell adhesion PDB id
1qz7

 

 

 

 

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Contents
Protein chains
524 a.a. *
17 a.a. *
Waters ×31
* Residue conservation analysis
PDB id:
1qz7
Name: Cell adhesion
Title: Beta-catenin binding domain of axin in complex with beta-catenin
Structure: Beta-catenin. Chain: a. Fragment: armadillo repeat region. Synonym: pro2286. Engineered: yes. Axin. Chain: b. Fragment: beta-catenin binding domain. Synonym: axis inhibition protein, xaxin.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ctnnb1. Expressed in: escherichia coli. Expression_system_taxid: 562. Xenopus laevis. African clawed frog. Organism_taxid: 8355.
Biol. unit: Tetramer (from PQS)
Resolution:
2.20Å     R-factor:   0.221     R-free:   0.256
Authors: Y.Xing,W.K.Clements,D.Kimelman,W.Xu
Key ref: Y.Xing et al. (2003). Crystal structure of a beta-catenin/axin complex suggests a mechanism for the beta-catenin destruction complex. Genes Dev, 17, 2753-2764. PubMed id: 14600025
Date:
15-Sep-03     Release date:   18-Nov-03    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P35222  (CTNB1_HUMAN) -  Catenin beta-1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		781 a.a.
524 a.a.
Protein chain
Pfam   ArchSchema ?
Q9YGY0  (AXIN1_XENLA) -  Axin-1 from Xenopus laevis
Seq:
Struc: 		 
Seq:
Struc: 		842 a.a.
17 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
Genes Dev 17:2753-2764 (2003)
PubMed id: 14600025  
 
 
Crystal structure of a beta-catenin/axin complex suggests a mechanism for the beta-catenin destruction complex.
Y.Xing, W.K.Clements, D.Kimelman, W.Xu.
 
  ABSTRACT  
 
The "beta-catenin destruction complex" is central to canonical Wnt/beta-catenin signaling. The scaffolding protein Axin and the tumor suppressor adenomatous polyposis coli protein (APC) are critical components of this complex, required for rapid beta-catenin turnover. We determined the crystal structure of a complex between beta-catenin and the beta-catenin-binding domain of Axin (Axin-CBD). The Axin-CBD forms a helix that occupies the groove formed by the third and fourth armadillo repeats of beta-catenin and thus precludes the simultaneous binding of other beta-catenin partners in this region. Our biochemical studies demonstrate that, when phosphorylated, the 20-amino acid repeat region of APC competes with Axin for binding to beta-catenin. We propose that a key function of APC in the beta-catenin destruction complex is to remove phosphorylated beta-catenin product from the active site.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20956359 G.Drechsel, J.Bergler, K.Wippel, N.Sauer, K.Vogelmann, and S.Hoth (2011).
C-terminal armadillo repeats are essential and sufficient for association of the plant U-box armadillo E3 ubiquitin ligase SAUL1 with the plasma membrane.
  J Exp Bot, 62, 775-785.  
19966865 E.M.Kohler, K.Brauburger, J.Behrens, and J.Schneikert (2010).
Contribution of the 15 amino acid repeats of truncated APC to beta-catenin degradation and selection of APC mutations in colorectal tumours from FAP patients.
  Oncogene, 29, 1663-1671.  
20883218 M.Adamska, C.Larroux, M.Adamski, K.Green, E.Lovas, D.Koop, G.S.Richards, C.Zwafink, and B.M.Degnan (2010).
Structure and expression of conserved Wnt pathway components in the demosponge Amphimedon queenslandica.
  Evol Dev, 12, 494-518.  
19850033 S.A.Kremer, N.Erdeniz, W.Peterson-Nedry, E.A.Swanson, and M.Wehrli (2010).
In vivo analysis in Drosophila reveals differential requirements of contact residues in Axin for interactions with GSK3beta or beta-catenin.
  Dev Biol, 337, 110-123.  
19619488 B.T.MacDonald, K.Tamai, and X.He (2009).
Wnt/beta-catenin signaling: components, mechanisms, and diseases.
  Dev Cell, 17, 9.  
19520846 C.Y.Ou, J.H.Kim, C.K.Yang, and M.R.Stallcup (2009).
Requirement of cell cycle and apoptosis regulator 1 for target gene activation by Wnt and beta-catenin and for anchorage-independent growth of human colon carcinoma cells.
  J Biol Chem, 284, 20629-20637.  
19131971 H.K.Arnold, X.Zhang, C.J.Daniel, D.Tibbitts, J.Escamilla-Powers, A.Farrell, S.Tokarz, C.Morgan, and R.C.Sears (2009).
The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.
  EMBO J, 28, 500-512.  
19204372 I.V.Chia, M.J.Kim, K.Itoh, S.Y.Sokol, and F.Costantini (2009).
Both the RGS domain and the six C-terminal amino acids of mouse Axin are required for normal embryogenesis.
  Genetics, 181, 1359-1368.  
  20066091 K.M.Cadigan, and M.Peifer (2009).
Wnt signaling from development to disease: insights from model systems.
  Cold Spring Harbor Perspect Biol, 1, a002881.  
19706613 R.Mo, T.L.Chew, M.T.Maher, G.Bellipanni, E.S.Weinberg, and C.J.Gottardi (2009).
The terminal region of beta-catenin promotes stability by shielding the Armadillo repeats from the axin-scaffold destruction complex.
  J Biol Chem, 284, 28222-28231.  
18432252 A.Klaus, and W.Birchmeier (2008).
Wnt signalling and its impact on development and cancer.
  Nat Rev Cancer, 8, 387-398.  
18359618 B.M.McCartney, and I.S.Näthke (2008).
Cell regulation by the Apc protein Apc as master regulator of epithelia.
  Curr Opin Cell Biol, 20, 186-193.  
18606007 C.Perez-Plasencia, A.Duenas-Gonzalez, and B.Alatorre-Tavera (2008).
Second hit in cervical carcinogenesis process: involvement of wnt/beta catenin pathway.
  Int Arch Med, 1, 10.  
18641688 G.Upadhyay, W.Goessling, T.E.North, R.Xavier, L.I.Zon, and V.Yajnik (2008).
Molecular association between beta-catenin degradation complex and Rac guanine exchange factor DOCK4 is essential for Wnt/beta-catenin signaling.
  Oncogene, 27, 5845-5855.  
18682750 I.M.Somorjai, and A.Martinez-Arias (2008).
Wingless signalling alters the levels, subcellular distribution and dynamics of Armadillo and E-cadherin in third instar larval wing imaginal discs.
  PLoS ONE, 3, e2893.  
18619997 M.S.Cortese, V.N.Uversky, and A.K.Dunker (2008).
Intrinsic disorder in scaffold proteins: getting more from less.
  Prog Biophys Mol Biol, 98, 85.  
18604449 X.Chen, J.Yang, P.M.Evans, and C.Liu (2008).
Wnt signaling: the good and the bad.
  Acta Biochim Biophys Sin (Shanghai), 40, 577-594.  
18172167 X.Guo, A.Ramirez, D.S.Waddell, Z.Li, X.Liu, and X.F.Wang (2008).
Axin and GSK3- control Smad3 protein stability and modulate TGF- signaling.
  Genes Dev, 22, 106-120.  
19061640 Y.Su, C.Fu, S.Ishikawa, A.Stella, M.Kojima, K.Shitoh, E.M.Schreiber, B.W.Day, and B.Liu (2008).
APC is essential for targeting phosphorylated beta-catenin to the SCFbeta-TrCP ubiquitin ligase.
  Mol Cell, 32, 652-661.  
18838381 Z.Lin, C.Gao, Y.Ning, X.He, W.Wu, and Y.G.Chen (2008).
The pseudoreceptor BMP and activin membrane-bound inhibitor positively modulates Wnt/beta-catenin signaling.
  J Biol Chem, 283, 33053-33058.  
17131344 I.C.Tsai, M.Woolf, D.W.Neklason, W.W.Branford, H.J.Yost, R.W.Burt, and D.M.Virshup (2007).
Disease-associated casein kinase I delta mutation may promote adenomatous polyps formation via a Wnt/beta-catenin independent mechanism.
  Int J Cancer, 120, 1005-1012.  
17660262 M.Ritco-Vonsovici, A.Ababou, and M.Horton (2007).
Molecular plasticity of beta-catenin: new insights from single-molecule measurements and MD simulation.
  Protein Sci, 16, 1984-1998.  
17928543 R.L.Daugherty, and C.J.Gottardi (2007).
Phospho-regulation of Beta-catenin adhesion and signaling functions.
  Physiology (Bethesda), 22, 303-309.  
17925400 S.Choi, E.A.Gustafson-Wagner, Q.Wang, S.M.Harlan, H.W.Sinn, J.L.Lin, and J.J.Lin (2007).
The intercalated disk protein, mXinalpha, is capable of interacting with beta-catenin and bundling actin filaments [corrected].
  J Biol Chem, 282, 36024-36036.  
17143292 D.Kimelman, and W.Xu (2006).
beta-catenin destruction complex: insights and questions from a structural perspective.
  Oncogene, 25, 7482-7491.  
16824055 F.Tang, Y.Peng, J.J.Nau, E.J.Kauffman, and L.S.Weisman (2006).
Vac8p, an armadillo repeat protein, coordinates vacuole inheritance with multiple vacuolar processes.
  Traffic, 7, 1368-1377.  
16952352 G.Zeng, U.Apte, A.Micsenyi, A.Bell, and S.P.Monga (2006).
Tyrosine residues 654 and 670 in beta-catenin are crucial in regulation of Met-beta-catenin interactions.
  Exp Cell Res, 312, 3620-3630.  
17081971 H.Clevers (2006).
Wnt/beta-catenin signaling in development and disease.
  Cell, 127, 469-480.  
16510874 J.Sierra, T.Yoshida, C.A.Joazeiro, and K.A.Jones (2006).
The APC tumor suppressor counteracts beta-catenin activation and H3K4 methylation at Wnt target genes.
  Genes Dev, 20, 586-600.  
15765408 J.N.Jensen, E.Cameron, M.V.Garay, T.W.Starkey, R.Gianani, and J.Jensen (2005).
Recapitulation of elements of embryonic development in adult mouse pancreatic regeneration.
  Gastroenterology, 128, 728-741.  
16051835 M.Fujimuro, J.Liu, J.Zhu, H.Yokosawa, and S.D.Hayward (2005).
Regulation of the interaction between glycogen synthase kinase 3 and the Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen.
  J Virol, 79, 10429-10441.  
15686623 R.Nusse (2005).
Wnt signaling in disease and in development.
  Cell Res, 15, 28-32.  
15473860 C.Y.Logan, and R.Nusse (2004).
The Wnt signaling pathway in development and disease.
  Annu Rev Cell Dev Biol, 20, 781-810.  
15327769 Y.Xing, W.K.Clements, I.Le Trong, T.R.Hinds, R.Stenkamp, D.Kimelman, and W.Xu (2004).
Crystal structure of a beta-catenin/APC complex reveals a critical role for APC phosphorylation in APC function.
  Mol Cell, 15, 523-533.
PDB code: 1th1
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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