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PDBsum entry 1qw0
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Metal binding protein
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PDB id
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1qw0
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Presence of ferric hydroxide clusters in mutants of haemophilus influenzae ferric ion-Binding protein a.
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Authors
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S.R.Shouldice,
R.J.Skene,
D.R.Dougan,
D.E.Mcree,
L.W.Tari,
A.B.Schryvers.
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Ref.
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Biochemistry, 2003,
42,
11908-11914.
[DOI no: ]
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PubMed id
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Abstract
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The periplasmic iron binding protein plays an essential role in the iron uptake
pathway of Gram-negative pathogenic bacteria from the Pasteurellaceae and
Neisseriaceae families and is critical for survival of these pathogens within
the host. In this study, we report the crystal structures of two mutant forms of
ferric ion-binding protein A (FbpA) from Haemophilus influenzae with bound
multinuclear oxo-metal clusters. Crystals of site-directed mutants in the metal
or anion binding ligands contain protein in the open conformation, and two
mutant FbpAs, H9A and N175L, contain different cluster arrangements in the
iron-binding pocket. The iron clusters are anchored by binding to the two
tyrosine ligands (Tyr195 and Tyr196) positioned at the vertex of the
iron-binding pocket but are not coordinated by the other metal binding ligands.
Our results suggest that the metal clusters may have formed in situ, suggesting
that the mutant FbpAs may serve as a simple model for protein-mediated
mineralization.
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