PDBsum entry 1qs7

Metal binding protein/peptide

PDB id: 1qs7

Contents

Protein chains

145 a.a. *

19 a.a. *

19 a.a. *

Metals

_CA ×8

Waters ×264

* Residue conservation analysis

PDB id:

1qs7

Name:

Metal binding protein/peptide

Title:

The 1.8 angstrom structure of calmodulin rs20 peptide complex

Structure:

Calmodulin. Chain: a, c. Engineered: yes. Rs20. Chain: b, d. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: synthetic construct. Synthetic: yes. Other_details: peptide analog of the calmodulin recognition region of chicken smooth muscle/ nonmuscle myosin light chain kinase

Biol. unit:

Dimer (from PQS)

Resolution:

1.80Å R-factor: 0.228 R-free: 0.259

Authors:

S.Weigand,W.F.Anderson

Key ref:

S.Weigand et al. High resolution structure of a calmodulin rs20 peptide complex. To be published, .

Date:

25-Jun-99 Release date: 24-Jun-03

Protein chains

A0MMD0  (A0MMD0_HORVU) -  EF-hand domain-containing protein from Hordeum vulgare

Seq: 149 a.a.

Struc: 145 a.a.*

Protein chain

P11799  (MYLK_CHICK) -  Myosin light chain kinase, smooth muscle from Gallus gallus

Seq: 1906 a.a.

Struc: 19 a.a.*

Protein chain

P11799  (MYLK_CHICK) -  Myosin light chain kinase, smooth muscle from Gallus gallus

Seq: 1906 a.a.

Struc: 19 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains B, D: E.C.2.7.11.18 - [myosin light-chain] kinase.
• Reaction:
  1. L-seryl-[myosin light chain] + ATP = O-phospho-L-seryl-[myosin light chain] + ADP + H⁺
  2. L-threonyl-[myosin light chain] + ATP = O-phospho-L-threonyl-[myosin light chain] + ADP + H⁺
• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site