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PDBsum entry 1qdw
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protein Protein-protein interface(s) links
Signaling protein PDB id
1qdw

 

 

 

 

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Contents
Protein chains
(+ 2 more) 87 a.a. *
Waters ×539
* Residue conservation analysis
PDB id:
1qdw
Name: Signaling protein
Title: N-terminal domain, voltage-gated potassium channel kv1.2 residues 33- 119
Structure: Kv1.2 voltage-gated potassium channel. Chain: a, b, c, d, e, f, g, h. Fragment: n-terminal domain kv1.2, residues 33-119 (core). Engineered: yes
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Organ: brain. Expressed in: escherichia coli. Expression_system_taxid: 562.
Biol. unit: Tetramer (from PQS)
Resolution:
2.10Å     R-factor:   0.223     R-free:   0.266
Authors: D.L.Minor Jr.,Y.-F.Lin,B.C.Mobley,A.Avelar,Y.N.Jan,L.Y.Jan,J.M.Berger
Key ref:
D.L.Minor et al. (2000). The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell, 102, 657-670. PubMed id: 11007484 DOI: 10.1016/S0092-8674(00)00088-X
Date:
10-Jul-99     Release date:   20-Sep-00    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P63142  (KCNA2_RAT) -  Potassium voltage-gated channel subfamily A member 2 from Rattus norvegicus
Seq:
Struc: 		499 a.a.
87 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
DOI no: 10.1016/S0092-8674(00)00088-X Cell 102:657-670 (2000)
PubMed id: 11007484  
 
 
The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel.
D.L.Minor, Y.F.Lin, B.C.Mobley, A.Avelar, Y.N.Jan, L.Y.Jan, J.M.Berger.
 
  ABSTRACT  
 
Kv voltage-gated potassium channels share a cytoplasmic assembly domain, T1. Recent mutagenesis of two T1 C-terminal loop residues implicates T1 in channel gating. However, structural alterations of these mutants leave open the question concerning direct involvement of T1 in gating. We find in mammalian Kv1.2 that gating depends critically on residues at complementary T1 surfaces in an unusually polar interface. An isosteric mutation in this interface causes surprisingly little structural alteration while stabilizing the closed channel and increasing the stability of T1 tetramers. Replacing T1 with a tetrameric coiled-coil destabilizes the closed channel. Together, these data suggest that structural changes involving the buried polar T1 surfaces play a key role in the conformational changes leading to channel opening.
 
  Selected figure(s)  
 
Figure 5.
Figure 5. T1 Interface Mutations That Affect Gating Cluster into “Hot Spots” on the Interface Surface(A) Molecular surface, cutaway view of the T1 tetramer seen from the side with one subunit deleted. “Side A” and “side B” of the interface are indicated. Residues are color-coded according to their effects on channel gating. Red indicates ΔV[1/2] of ≥ |5 mV|, green indicates no significant change, and purple indicates residues intolerant to change.(B) and (C), external views of the T1 tetramer revealing the accessibility of hot spot residues from the exterior of T1. 		Figure 7.
Figure 7. Cartoon of an Intact Kv ChannelThe 4-fold axis in T1 is aligned with the 4-fold axis presumed to run through the channel pore. Transmembrane segments and C-terminal domains are indicated for two subunits and colored as in Figure 1. The green hexagon represents the N-terminal inactivation ball present in some Kv channels. Only one inactivation ball is shown for clarity.
 
  The above figures are reprinted by permission from Cell Press: Cell (2000, 102, 657-670) copyright 2000.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
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20018952 E.D.Burg, O.Platoshyn, I.F.Tsigelny, B.Lozano-Ruiz, B.K.Rana, and J.X.Yuan (2010).
Tetramerization domain mutations in KCNA5 affect channel kinetics and cause abnormal trafficking patterns.
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20065070 K.Howell, S.Arur, T.Schedl, and M.V.Sundaram (2010).
EOR-2 is an obligate binding partner of the BTB-zinc finger protein EOR-1 in Caenorhabditis elegans.
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19947938 L.L.Zhao, Z.Qi, X.E.Zhang, L.J.Bi, and G.Jin (2010).
Regulatory role of the extreme C-terminal end of the S6 inner helix in C-terminal-truncated Kv1.2 channel activation.
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19270700 A.Kosolapov, and C.Deutsch (2009).
Tertiary interactions within the ribosomal exit tunnel.
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18836841 A.V.Pischalnikova, and O.S.Sokolova (2009).
The domain and conformational organization in potassium voltage-gated ion channels.
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Conserved negative charges in the N-terminal tetramerization domain mediate efficient assembly of Kv2.1 and Kv2.1/Kv6.4 channels.
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19074135 M.Mederos Y Schnitzler, S.Rinné, L.Skrobek, V.Renigunta, G.Schlichthörl, C.Derst, T.Gudermann, J.Daut, and R.Preisig-Müller (2009).
Mutation of histidine 105 in the t1 domain of the potassium channel kv2.1 disrupts heteromerization with kv6.3 and kv6.4.
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19170764 N.Ito, M.Watanabe-Matsui, K.Igarashi, and K.Murayama (2009).
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Identification and Functional Characterization of Protein Kinase A-catalyzed Phosphorylation of Potassium Channel Kv1.2 at Serine 449.
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Intracellular domains interactions and gated motions of I(KS) potassium channel subunits.
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18221766 B.A.Wilton, S.Campbell, N.Van Buuren, R.Garneau, M.Furukawa, Y.Xiong, and M.Barry (2008).
Ectromelia virus BTB/kelch proteins, EVM150 and EVM167, interact with cullin-3-based ubiquitin ligases.
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18833322 C.J.Camacho (2008).
Quantitative modeling of currents from a voltage gated ion channel undergoing fast inactivation.
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18003609 E.C.Connors, B.A.Ballif, and A.D.Morielli (2008).
Homeostatic Regulation of Kv1.2 Potassium Channel Trafficking by Cyclic AMP.
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18357523 M.Covarrubias, A.Bhattacharji, J.A.De Santiago-Castillo, K.Dougherty, Y.A.Kaulin, T.R.Na-Phuket, and G.Wang (2008).
The neuronal Kv4 channel complex.
  Neurochem Res, 33, 1558-1567.  
18334215 S.Chakrapani, L.G.Cuello, D.M.Cortes, and E.Perozo (2008).
Structural dynamics of an isolated voltage-sensor domain in a lipid bilayer.
  Structure, 16, 398-409.  
18989792 S.I.Börjesson, and F.Elinder (2008).
Structure, function, and modification of the voltage sensor in voltage-gated ion channels.
  Cell Biochem Biophys, 52, 149-174.  
18782578 Y.Fujiwara, and D.L.Minor (2008).
X-ray crystal structure of a TRPM assembly domain reveals an antiparallel four-stranded coiled-coil.
  J Mol Biol, 383, 854-870.
PDB code: 3e7k
19016844 Z.Yuchi, V.P.Pau, and D.S.Yang (2008).
GCN4 enhances the stability of the pore domain of potassium channel KcsA.
  FEBS J, 275, 6228-6236.  
17908685 C.B.Phelps, and R.Gaudet (2007).
The role of the N terminus and transmembrane domain of TRPM8 in channel localization and tetramerization.
  J Biol Chem, 282, 36474-36480.  
17984060 H.Yang, L.Hu, J.Shi, K.Delaloye, F.T.Horrigan, and J.Cui (2007).
Mg2+ mediates interaction between the voltage sensor and cytosolic domain to activate BK channels.
  Proc Natl Acad Sci U S A, 104, 18270-18275.  
18056633 J.W.Yang, H.Vacher, K.S.Park, E.Clark, and J.S.Trimmer (2007).
Trafficking-dependent phosphorylation of Kv1.2 regulates voltage-gated potassium channel cell surface expression.
  Proc Natl Acad Sci U S A, 104, 20055-20060.  
17766348 S.Rezazadeh, H.T.Kurata, T.W.Claydon, S.J.Kehl, and D.Fedida (2007).
An activation gating switch in Kv1.2 is localized to a threonine residue in the S2-S3 linker.
  Biophys J, 93, 4173-4186.  
  16735758 A.A.Fodor, and R.W.Aldrich (2006).
Statistical limits to the identification of ion channel domains by sequence similarity.
  J Gen Physiol, 127, 755-766.  
16909197 A.F.Dulhunty, N.A.Beard, P.Pouliquin, and T.Kimura (2006).
Novel regulators of RyR Ca2+ release channels: insight into molecular changes in genetically-linked myopathies.
  J Muscle Res Cell Motil, 27, 351-365.  
16690619 E.Kobrinsky, L.Stevens, Y.Kazmi, D.Wray, and N.M.Soldatov (2006).
Molecular rearrangements of the Kv2.1 potassium channel termini associated with voltage gating.
  J Biol Chem, 281, 19233-19240.  
  16533897 G.Wang, and M.Covarrubias (2006).
Voltage-dependent gating rearrangements in the intracellular T1-T1 interface of a K+ channel.
  J Gen Physiol, 127, 391-400.  
17057713 M.Pioletti, F.Findeisen, G.L.Hura, and D.L.Minor (2006).
Three-dimensional structure of the KChIP1-Kv4.3 T1 complex reveals a cross-shaped octamer.
  Nat Struct Mol Biol, 13, 987-995.
PDB code: 2i2r
16725367 P.Pouliquin, S.M.Pace, S.M.Curtis, P.J.Harvey, E.M.Gallant, F.Zorzato, M.G.Casarotto, and A.F.Dulhunty (2006).
Effects of an alpha-helical ryanodine receptor C-terminal tail peptide on ryanodine receptor activity: modulation by Homer.
  Int J Biochem Cell Biol, 38, 1700-1715.  
16846855 P.R.Tsuruda, D.Julius, and D.L.Minor (2006).
Coiled coils direct assembly of a cold-activated TRP channel.
  Neuron, 51, 201-212.  
16267500 R.Jalkanen, E.Pronicka, H.Tyynismaa, A.Hanauer, R.Walder, and T.Alitalo (2006).
Genetic background of HSH in three Polish families and a patient with an X;9 translocation.
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17468960 R.Wang, J.Su, X.Zhang, Y.Shi, N.Cui, V.A.Onyebuchi, and C.Jiang (2006).
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  J Membr Biol, 213, 155-164.  
16857707 Y.Fujiwara, and Y.Kubo (2006).
Regulation of the desensitization and ion selectivity of ATP-gated P2X2 channels by phosphoinositides.
  J Physiol, 576, 135-149.  
15827117 D.Kerschensteiner, F.Soto, and M.Stocker (2005).
Fluorescence measurements reveal stoichiometry of K+ channels formed by modulatory and delayed rectifier alpha-subunits.
  Proc Natl Acad Sci U S A, 102, 6160-6165.  
  15955876 G.Wang, M.Shahidullah, C.A.Rocha, C.Strang, P.J.Pfaffinger, and M.Covarrubias (2005).
Functionally active t1-t1 interfaces revealed by the accessibility of intracellular thiolate groups in kv4 channels.
  J Gen Physiol, 126, 55-69.  
15953418 H.L.Agler, J.Evans, L.H.Tay, M.J.Anderson, H.M.Colecraft, and D.T.Yue (2005).
G protein-gated inhibitory module of N-type (ca(v)2.2) ca2+ channels.
  Neuron, 46, 891-904.  
16262625 J.F.Rivera, P.J.Chu, and D.B.Arnold (2005).
The T1 domain of Kv1.3 mediates intracellular targeting to axons.
  Eur J Neurosci, 22, 1853-1862.  
15664174 J.M.Robinson, and C.Deutsch (2005).
Coupled tertiary folding and oligomerization of the T1 domain of Kv channels.
  Neuron, 45, 223-232.  
15961040 K.Heusser, and B.Schwappach (2005).
Trafficking of potassium channels.
  Curr Opin Neurobiol, 15, 364-369.  
16179364 K.Nakajo, and Y.Kubo (2005).
Protein kinase C shifts the voltage dependence of KCNQ/M channels expressed in Xenopus oocytes.
  J Physiol, 569, 59-74.  
16207353 P.J.Stogios, G.S.Downs, J.J.Jauhal, S.K.Nandra, and G.G.Privé (2005).
Sequence and structural analysis of BTB domain proteins.
  Genome Biol, 6, R82.  
  15618483 S.C.Hebert, G.Desir, G.Giebisch, and W.Wang (2005).
Molecular diversity and regulation of renal potassium channels.
  Physiol Rev, 85, 319-371.  
15816169 S.J.Korn, and J.G.Trapani (2005).
Potassium channels.
  IEEE Trans Nanobioscience, 4, 21-33.  
15781472 S.Matsuda, Y.Kamiya, and M.Yuzaki (2005).
Roles of the N-terminal domain on the function and quaternary structure of the ionotropic glutamate receptor.
  J Biol Chem, 280, 20021-20029.  
16000151 Y.Tao, R.Zeng, B.Shen, J.Jia, and Y.Wang (2005).
Neuronal transmission stimulates the phosphorylation of Kv1.4 channel at Ser229 through protein kinase A1.
  J Neurochem, 94, 1512-1522.  
15473968 A.Kosolapov, L.Tu, J.Wang, and C.Deutsch (2004).
Structure acquisition of the T1 domain of Kv1.3 during biogenesis.
  Neuron, 44, 295-307.  
15298895 A.Scholle, T.Zimmer, R.Koopmann, B.Engeland, O.Pongs, and K.Benndorf (2004).
Effects of Kv1.2 intracellular regions on activation of Kv2.1 channels.
  Biophys J, 87, 873-882.  
14645239 N.Hatano, S.Ohya, K.Muraki, R.B.Clark, W.R.Giles, and Y.Imaizumi (2004).
Two arginines in the cytoplasmic C-terminal domain are essential for voltage-dependent regulation of A-type K+ current in the Kv4 channel subfamily.
  J Biol Chem, 279, 5450-5459.  
15489237 Q.Chang, E.Gyftogianni, S.F.van de Graaf, S.Hoefs, F.A.Weidema, R.J.Bindels, and J.G.Hoenderop (2004).
Molecular determinants in TRPV5 channel assembly.
  J Biol Chem, 279, 54304-54311.  
14976260 V.Chubanov, S.Waldegger, M.Mederos y Schnitzler, H.Vitzthum, M.C.Sassen, H.W.Seyberth, M.Konrad, and T.Gudermann (2004).
Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia.
  Proc Natl Acad Sci U S A, 101, 2894-2899.  
12431984 A.Kosolapov, and C.Deutsch (2003).
Folding of the voltage-gated K+ channel T1 recognition domain.
  J Biol Chem, 278, 4305-4313.  
12893943 C.Gu, Y.N.Jan, and L.Y.Jan (2003).
A conserved domain in axonal targeting of Kv1 (Shaker) voltage-gated potassium channels.
  Science, 301, 646-649.  
12554641 M.Jenke, A.Sánchez, F.Monje, W.Stühmer, R.M.Weseloh, and L.A.Pardo (2003).
C-terminal domains implicated in the functional surface expression of potassium channels.
  EMBO J, 22, 395-403.  
12560340 M.Ju, L.Stevens, E.Leadbitter, and D.Wray (2003).
The Roles of N- and C-terminal determinants in the activation of the Kv2.1 potassium channel.
  J Biol Chem, 278, 12769-12778.  
14569011 O.Sokolova, A.Accardi, D.Gutierrez, A.Lau, M.Rigney, and N.Grigorieff (2003).
Conformational changes in the C terminus of Shaker K+ channel bound to the rat Kvbeta2-subunit.
  Proc Natl Acad Sci U S A, 100, 12607-12612.  
12551944 P.Koprowski, and A.Kubalski (2003).
C termini of the Escherichia coli mechanosensitive ion channel (MscS) move apart upon the channel opening.
  J Biol Chem, 278, 11237-11245.  
12615930 Z.Tiran, A.Peretz, B.Attali, and A.Elson (2003).
Phosphorylation-dependent regulation of Kv2.1 Channel activity at tyrosine 124 by Src and by protein-tyrosine phosphatase epsilon.
  J Biol Chem, 278, 17509-17514.  
12372826 A.W.Jahng, C.Strang, D.Kaiser, T.Pollard, P.Pfaffinger, and S.Choe (2002).
Zinc mediates assembly of the T1 domain of the voltage-gated K channel 4.2.
  J Biol Chem, 277, 47885-47890.  
11826262 C.Deutsch (2002).
Potassium channel ontogeny.
  Annu Rev Physiol, 64, 19-46.  
12021261 H.T.Kurata, G.S.Soon, J.R.Eldstrom, G.W.Lu, D.F.Steele, and D.Fedida (2002).
Amino-terminal determinants of U-type inactivation of voltage-gated K+ channels.
  J Biol Chem, 277, 29045-29053.  
12062021 J.J.Rosenthal, and F.Bezanilla (2002).
Extensive editing of mRNAs for the squid delayed rectifier K+ channel regulates subunit tetramerization.
  Neuron, 34, 743-757.  
12539258 S.Choe, and T.Roosild (2002).
Regulation of the K channels by cytoplasmic domains.
  Biopolymers, 66, 294-299.  
11854493 S.H.Loukin, J.Lin, U.Athar, C.Palmer, and Y.Saimi (2002).
The carboxyl tail forms a discrete functional domain that blocks closure of the yeast K+ channel.
  Proc Natl Acad Sci U S A, 99, 1926-1930.  
11806920 S.H.Loukin, and Y.Saimi (2002).
Carboxyl tail prevents yeast K(+) channel closure: proposal of an integrated model of TOK1 gating.
  Biophys J, 82, 781-792.  
11572962 B.A.Yi, D.L.Minor, Y.F.Lin, Y.N.Jan, and L.Y.Jan (2001).
Controlling potassium channel activities: Interplay between the membrane and intracellular factors.
  Proc Natl Acad Sci U S A, 98, 11016-11023.  
11432987 E.Aydar, and C.Palmer (2001).
Functional characterization of the C-terminus of the human ether-à-go-go-related gene K(+) channel (HERG).
  J Physiol, 534, 1.  
  11524461 H.T.Kurata, G.S.Soon, and D.Fedida (2001).
Altered state dependence of c-type inactivation in the long and short forms of human Kv1.5.
  J Gen Physiol, 118, 315-332.  
11598005 M.F.Rosenberg, G.Velarde, R.C.Ford, C.Martin, G.Berridge, I.D.Kerr, R.Callaghan, A.Schmidlin, C.Wooding, K.J.Linton, and C.F.Higgins (2001).
Repacking of the transmembrane domains of P-glycoprotein during the transport ATPase cycle.
  EMBO J, 20, 5615-5625.  
11286888 O.Sokolova, L.Kolmakova-Partensky, and N.Grigorieff (2001).
Three-dimensional structure of a voltage-gated potassium channel at 2.5 nm resolution.
  Structure, 9, 215-220.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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