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PDBsum entry 1q7f
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Model of the brain tumor-Pumilio translation repressor complex.
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Authors
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T.A.Edwards,
B.D.Wilkinson,
R.P.Wharton,
A.K.Aggarwal.
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Ref.
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Genes Dev, 2003,
17,
2508-2513.
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PubMed id
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Abstract
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The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR)
of hunchback mRNA to regulate its translation. Recruitment is mediated by
interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of
Brat, a conserved structural motif present in a large family of growth
regulators. In this report, we describe the crystal structure of the Brat NHL
domain and present a model of the Pumilio-Brat complex derived from in silico
docking experiments and supported by mutational analysis of the protein-protein
interface. A key feature of the model is recognition of the outer, convex
surface of the Pumilio Puf domain by the top, electropositive face of the
six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8
fits in the entrance to the central channel of the Brat beta-propeller.
Together, these interactions are likely to be prototypic of the recruitment
strategies of other NHL-containing proteins in development.
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