PDBsum entry 1pvu

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Type ii restriction endonuclease PDB id
Protein chains
154 a.a.
Waters ×79

References listed in PDB file
Key reference
Title Crystal structure of pvuii endonuclease reveals extensive structural homologies to ecorv.
Authors A.Athanasiadis, M.Vlassi, D.Kotsifaki, P.A.Tucker, K.S.Wilson, M.Kokkinidis.
Ref. Nat Struct Biol, 1994, 1, 469-475.
PubMed id 7664066
Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 95%.
The crystal structure of the dimeric PvuII restriction endonuclease (R.PvuII) has been determined at a resolution of 2.4A. The protein has a mixed alpha/beta architecture and consists of two subdomains. Despite a lack of sequence homology, extensive structural similarities exist between one R.PvuII subdomain and the DNA-binding subdomain of EcoRV endonuclease (R.EcoRV); the dimerization subdomains are unrelated. Within the similar domains, flexible segments of R.PvuII are topologically equivalent to the DNA-binding turns of R.EcoRV; potential catalytic residues can be deduced from the structural similarities to R.EcoRV. Conformational flexibility is important for the interaction with DNA. A possible classification of endonuclease structures on the basis of the positions of the scissile phosphates is discussed.
Secondary reference #1
Title Purification, Crystallization and preliminary X-Ray diffraction studies of the pvuii endonuclease.
Authors A.Athanasiadis, M.Kokkinidis.
Ref. J Mol Biol, 1991, 222, 451-453.
PubMed id 1748988
Secondary reference #2
Title Complete nucleotide sequence of the pvuii restriction enzyme gene from proteus vulgaris.
Authors A.Athanasiadis, M.Gregoriu, D.Thanos, M.Kokkinidis, J.Papamatheakis.
Ref. Nucleic Acids Res, 1990, 18, 6434.
PubMed id 2243794
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