spacer
spacer

PDBsum entry 1pvb

Go to PDB code: 
Top Page protein ligands metals links
Calcium binding protein PDB id
1pvb
Contents
Protein chain
108 a.a.
Ligands
NH4
Metals
_CA ×2
Waters ×72

References listed in PDB file
Key reference
Title X-Ray structure of a new crystal form of pike 4.10 beta parvalbumin.
Authors J.P.Declercq, B.Tinant, J.Parello.
Ref. Acta Crystallogr D Biol Crystallogr, 1996, 52, 165-169. [DOI no: 10.1107/S0907444995010006]
PubMed id 15299738
Note In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above were identified by an automated search of PubMed on title and author names, giving a percentage match of 94%.
Abstract
A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in presence of EDTA at pH 8.0. The crystals are orthorhombic, space group P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data were collected to 1.75 A. The structure was solved by molecular replacement and in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the previously reported structures, the main differences concern the conformation of the N-terminal residues and the packing in the unit cell.
Figure 5.
Fig. 5. Superposition of the Ca atoms of pike (p/4.10) parvalbumin crystallized in the presence of EDTA (this study, thick lines) and in the absence of EDTA [1PAL, Declercq et al. (1991), thin lines].
Figure 5.
Fig. 5. Superposition of the Ca atoms of pike (p/4.10) parvalbumin crystallized in the presence of EDTA (this study, thick lines) and in the absence of EDTA [1PAL, Declercq et al. (1991), thin lines].
The above figures are reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (1996, 52, 165-169) copyright 1996.
Secondary reference #1
Title Ionic interactions with parvalbumins. Crystal structure determination of pike 4.10 parvalbumin in four different ionic environments.
Authors J.P.Declercq, B.Tinant, J.Parello, J.Rambaud.
Ref. J Mol Biol, 1991, 220, 1017-1039.
PubMed id 1880797
Abstract
Secondary reference #2
Title Crystal structure determination and refinement of pike 4.10 parvalbumin (minor component from esox lucius).
Authors J.P.Declercq, B.Tinant, J.Parello, G.Etienne, R.Huber.
Ref. J Mol Biol, 1988, 202, 349-353.
PubMed id 3172221
Abstract
PROCHECK
Go to PROCHECK summary
 Headers

 

spacer

spacer