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PDBsum entry 1ppf
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Hydrolase/hydrolase inhibitor
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PDB id
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1ppf
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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X-Ray crystal structure of the complex of human leukocyte elastase (pmn elastase) and the third domain of the turkey ovomucoid inhibitor.
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Authors
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W.Bode,
A.Z.Wei,
R.Huber,
E.Meyer,
J.Travis,
S.Neumann.
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Ref.
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Embo J, 1986,
5,
2453-2458.
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PubMed id
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Abstract
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Orthorhombic crystals diffracting beyond 1.7 A resolution, have been grown from
the stoichiometric complex formed between human leukocyte elastase (HLE) and the
third domain of turkey ovomucoid inhibitor (OMTKY3). The crystal and molecular
structure has been determined with the multiple isomorphous replacement
technique. The complex has been modeled using the known structure of OMTKY3 and
partial sequence information for HLE, and has been refined. The current
crystallographic R-value is 0.21 for reflections from 25 to 1.8 A resolution.
HLE shows the characteristic polypeptide fold of trypsin-like serine proteinases
and consists of 218 amino acid residues. However, several loop segments, mainly
arranged around the substrate binding site, have unique conformations. The
largest deviations from the other vertebrate proteinases of known spatial
structure are around Cys168. The specificity pocket is constricted by Val190,
Val216 and Asp226 to preferentially accommodate medium sized hydrophobic amino
acids at P1. Seven residues of the OMTKY3-binding segment are in specific
contact with HLE. This interaction and geometry around the reactive site are
similar as observed in other complexes. It is the first serine proteinase
glycoprotein analysed, having two sugar chains attached to Asn159 and to residue
109.
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Secondary reference #1
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Title
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Authors
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W.Bode,
A.Z.Wei,
M.Stubbs,
M.Laskowski.
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Ref.
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TO BE PUBLISHED ...
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Secondary reference #2
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Title
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Human leukocyte and porcine pancreatic elastase: X-Ray crystal structures, Mechanism, Substrate specificity, And mechanism-Based inhibitors.
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Authors
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W.Bode,
E.Meyer,
J.C.Powers.
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Ref.
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Biochemistry, 1989,
28,
1951-1963.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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The refined 2.3 a crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor.
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Authors
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A.Z.Wei,
I.Mayr,
W.Bode.
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Ref.
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FEBS Lett, 1988,
234,
367-373.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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Primary structure of human neutrophil elastase.
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Authors
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S.Sinha,
W.Watorek,
S.Karr,
J.Giles,
W.Bode,
J.Travis.
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Ref.
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Proc Natl Acad Sci U S A, 1987,
84,
2228-2232.
[DOI no: ]
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PubMed id
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