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PDBsum entry 1poc
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References listed in PDB file
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Key reference
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Title
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Crystal structure of bee-Venom phospholipase a2 in a complex with a transition-State analogue.
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Authors
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D.L.Scott,
Z.Otwinowski,
M.H.Gelb,
P.B.Sigler.
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Ref.
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Science, 1990,
250,
1563-1566.
[DOI no: ]
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PubMed id
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Abstract
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The 2.0 angstroms crystal structure of a complex containing bee-venom
phospholipase A2 (PLA2) and a phosphonate transition-state analogue was solved
by multiple isomorphous replacement. The electron-density map is sufficiently
detailed to visualize the proximal sugars of the enzyme's N-linked carbohydrate
and a single molecule of the transition-state analogue bound ot its active
center. Although bee-venom PLA2 does not belong to the large homologous Class
I/II family that encompasses most other well-studied PLA2s, there is segmental
sequence similarity and conservation of many functional substructures.
Comparison of the bee-venom enzyme with other phospholipase structures provides
compelling evidence for a common catalytic mechanism.
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Secondary reference #1
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Title
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Interfacial catalysis: the mechanism of phospholipase a2.
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Authors
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D.L.Scott,
S.P.White,
Z.Otwinowski,
W.Yuan,
M.H.Gelb,
P.B.Sigler.
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Ref.
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Science, 1990,
250,
1541-1546.
[DOI no: ]
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PubMed id
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