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PDBsum entry 1pnj
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Phosphotransferase
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PDB id
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1pnj
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References listed in PDB file
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Key reference
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Title
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Solution structure and ligand-Binding site of the sh3 domain of the p85 alpha subunit of phosphatidylinositol 3-Kinase.
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Authors
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G.W.Booker,
I.Gout,
A.K.Downing,
P.C.Driscoll,
J.Boyd,
M.D.Waterfield,
I.D.Campbell.
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Ref.
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Cell, 1993,
73,
813-822.
[DOI no: ]
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PubMed id
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Abstract
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SH3 domains are found in proteins associated with receptor tyrosine kinase
signal transduction complexes. The solution structure of the SH3 domain of the
85 kd regulatory subunit of phosphatidylinositol 3-kinase is shown to be a
compact beta barrel consisting of five beta strands arranged in two beta sheets
of three and two strands. The structure is similar to that of chicken brain
alpha spectrin but represents a distinct class of SH3 domain, with an insertion
between the second and third beta strands that may influence binding
specificity. 1H chemical shift changes induced by complex formation with a
synthetic peptide derived from the SH3-binding protein dynamin, together with
amino acid sequence comparisons, suggest that the ligand-binding site consists
of a hydrophobic surface flanked by two charged loops.
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