UniProt functional annotation for P01096



	UniProt functional annotation for P01096

UniProt code: P01096.

Organism: Bos taurus (Bovine).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.

Function: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. {ECO:0000269|PubMed:10831597, ECO:0000269|PubMed:12923572, ECO:0000269|PubMed:17895376, ECO:0000269|PubMed:18687699, ECO:0000269|PubMed:21192948, ECO:0000269|PubMed:7397110}.

Subunit: Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0. {ECO:0000269|PubMed:10831597, ECO:0000269|PubMed:11742976, ECO:0000269|PubMed:12923572, ECO:0000269|PubMed:17895376, ECO:0000269|PubMed:18687699, ECO:0000269|PubMed:21192948}.

Subcellular location: Mitochondrion {ECO:0000269|PubMed:7397110}.

Domain: Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 74-106, leaving each N-terminal inhibitory region (residues 26-62) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 26-62) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (PubMed:11742976, PubMed:12923572 and PubMed:17895376).

Similarity: Belongs to the ATPase inhibitor family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bos taurus (Bovine).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.



Function:		Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase. {ECO:0000269\|PubMed:10831597, ECO:0000269\|PubMed:12923572, ECO:0000269\|PubMed:17895376, ECO:0000269\|PubMed:18687699, ECO:0000269\|PubMed:21192948, ECO:0000269\|PubMed:7397110}.


Subunit:		Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0. {ECO:0000269\|PubMed:10831597, ECO:0000269\|PubMed:11742976, ECO:0000269\|PubMed:12923572, ECO:0000269\|PubMed:17895376, ECO:0000269\|PubMed:18687699, ECO:0000269\|PubMed:21192948}.
Subcellular location:		Mitochondrion {ECO:0000269\|PubMed:7397110}.
Domain:		Forms an alpha-helical dimer with monomers associated via an antiparallel alpha-helical coiled coil composed of residues 74-106, leaving each N-terminal inhibitory region (residues 26-62) accessible for interaction with an F1 catalytic domain. The inhibitory N-terminal region (residues 26-62) binds the alpha(ADP-bound)-beta(ADP-bound) (ATP5F1A-ATP5F1B) interface of F1-ATPase, and also contact the central gamma subunit (ATP5F1C). This dimeric state is favored by pH values below 7.0, and at higher values the dimers associate to form inactive homotetramer, where the inhibitory region is occluded, masking its inhibitory activity (PubMed:11742976, PubMed:12923572 and PubMed:17895376).
Similarity:		Belongs to the ATPase inhibitor family. {ECO:0000305}.