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PDBsum entry 1oda
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Lectin/immune system
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PDB id
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1oda
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Lectin/immune system
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Title:
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N-terminal of sialoadhesin in complex with me-a-9-n-(biphenyl-4- carbonyl)-amino-9-deoxy-neu5ac (bip compound)
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Structure:
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Sialoadhesin. Chain: a. Fragment: domain one, ig-like v-type domain, residues 20-138. Synonym: snd1, sialic acid binding ig-like lectin-1, siglec-1. Engineered: yes. Other_details: bound to me-a-9-n-(biphenyl-4-carbonyl)-amino-9-deoxy- neu5ac, coordinate system origx origx matrix (m) origx vector (v)
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Source:
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Mus musculus. Mouse. Organism_taxid: 10090. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho cell. Other_details: cho cell stable expression with pee14 plasmid
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Resolution:
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3.31Å
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R-factor:
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0.217
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R-free:
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0.284
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Authors:
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N.R.Zaccai,K.Maenaka,T.Maenaka,P.R.Crocker,R.Brossmer,S.Kelm, E.Y.Jones
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Key ref:
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N.R.Zaccai
et al.
(2003).
Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin.
Structure,
11,
557-567.
PubMed id:
DOI:
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Date:
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14-Feb-03
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Release date:
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14-Aug-03
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PROCHECK
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Headers
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References
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Q62230
(SN_MOUSE) -
Sialoadhesin from Mus musculus
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Seq:
Struc:
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1695 a.a.
117 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Structure
11:557-567
(2003)
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PubMed id:
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Structure-guided design of sialic acid-based Siglec inhibitors and crystallographic analysis in complex with sialoadhesin.
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N.R.Zaccai,
K.Maenaka,
T.Maenaka,
P.R.Crocker,
R.Brossmer,
S.Kelm,
E.Y.Jones.
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ABSTRACT
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The Siglec family of receptors mediates cell surface interactions through
recognition of sialylated glycoconjugates. The crystal structure of the
N-terminal immunoglobulin-like domain of the Siglec sialoadhesin (SnD1) in
complex with 2,3-sialyllactose has informed the design of sialic acid analogs
(sialosides) that bind Siglecs with significantly enhanced affinities and
specificities. Binding assays against sialoadhesin (Sn; Siglec-1), CD22
(Siglec-2), and MAG (Siglec-4) show a 10- to 300-fold reduction in IC(50) values
(relative to methyl-alpha-Neu5Ac) for three sialosides bearing aromatic group
modifications of the glycerol side chain:
Me-alpha-9-N-benzoyl-amino-9-deoxy-Neu5Ac (BENZ),
Me-alpha-9-N-(naphthyl-2-carbonyl)-amino-9-deoxy-Neu5Ac (NAP), and
Me-alpha-9-N-(biphenyl-4-carbonyl)-amino-9-deoxy-Neu5Ac (BIP). Crystal
structures of these sialosides in complex with SnD1 suggest explanations for the
differences in specificity and affinity, providing further ideas for compound
design of physiological and potentially therapeutic relevance.
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Selected figure(s)
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Figure 1.
Figure 1. Chemical Structures of Sialosides Used in This
Study
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The above figure is
reprinted
by permission from Cell Press:
Structure
(2003,
11,
557-567)
copyright 2003.
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Figure was
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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G.J.Boons
(2010).
Liposomes modified by carbohydrate ligands can target B cells for the treatment of B-cell lymphomas.
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Expert Rev Vaccines,
9,
1251-1256.
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L.Nitschke
(2009).
CD22 and Siglec-G: B-cell inhibitory receptors with distinct functions.
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Immunol Rev,
230,
128-143.
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E.L.Goh,
J.K.Young,
K.Kuwako,
M.Tessier-Lavigne,
Z.He,
J.W.Griffin,
and
G.L.Ming
(2008).
beta1-integrin mediates myelin-associated glycoprotein signaling in neuronal growth cones.
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Mol Brain,
1,
10.
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O.Blixt,
S.Han,
L.Liao,
Y.Zeng,
J.Hoffmann,
S.Futakawa,
and
J.C.Paulson
(2008).
Sialoside analogue arrays for rapid identification of high affinity siglec ligands.
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J Am Chem Soc,
130,
6680-6681.
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M.Nowicki,
J.Kosacka,
R.Brossmer,
K.Spanel-Borowski,
and
J.Borlak
(2007).
The myelin-associated glycoprotein inhibitor BENZ induces outgrowth and survival of rat dorsal root ganglion cell cultures.
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J Neurosci Res,
85,
3053-3063.
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P.R.Crocker,
J.C.Paulson,
and
A.Varki
(2007).
Siglecs and their roles in the immune system.
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Nat Rev Immunol,
7,
255-266.
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E.M.Rapoport,
G.V.Pazynina,
M.A.Sablina,
P.R.Crocker,
and
N.V.Bovin
(2006).
Probing sialic acid binding Ig-like lectins (siglecs) with sulfated oligosaccharides.
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Biochemistry (Mosc),
71,
496-504.
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A.A.Vyas,
O.Blixt,
J.C.Paulson,
and
R.L.Schnaar
(2005).
Potent glycan inhibitors of myelin-associated glycoprotein enhance axon outgrowth in vitro.
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J Biol Chem,
280,
16305-16310.
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S.Han,
B.E.Collins,
P.Bengtson,
and
J.C.Paulson
(2005).
Homomultimeric complexes of CD22 in B cells revealed by protein-glycan cross-linking.
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Nat Chem Biol,
1,
93-97.
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Y.Ikehara,
S.K.Ikehara,
and
J.C.Paulson
(2004).
Negative regulation of T cell receptor signaling by Siglec-7 (p70/AIRM) and Siglec-9.
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J Biol Chem,
279,
43117-43125.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
