 |
PDBsum entry 1oct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Transcription/DNA
|
PDB id
|
|
|
|
1oct
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Crystal structure of the oct-1 pou domain bound to an octamer site: DNA recognition with tethered DNA-Binding modules.
|
|
|
Authors
|
|
J.D.Klemm,
M.A.Rould,
R.Aurora,
W.Herr,
C.O.Pabo.
|
|
|
Ref.
|
|
Cell, 1994,
77,
21-32.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The structure of an Oct-1 POU domain-octamer DNA complex has been solved at 3.0
A resolution. The POU-specific domain contacts the 5' half of this site
(ATGCAAAT), and as predicted from nuclear magnetic resonance studies, the
structure, docking, and contacts are remarkably similar to those of the lambda
and 434 repressors. The POU homeodomain contacts the 3' half of this site
(ATGCAAAT), and the docking is similar to that of the engrailed, MAT alpha 2,
and Antennapedia homeodomains. The linker region is not visible and there are no
protein-protein contacts between the domains, but overlapping phosphate contacts
near the center of the octamer site may favor cooperative binding. This novel
arrangement raises important questions about cooperativity in protein-DNA
recognition.
|
|
|
|
|
|
|
