|
|
||
|
|
|
|
|
UniProt functional annotation for P33203 | ||
|
|
|
|
|
|
||
| UniProt code: P33203. |
|
||
| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
|
||
|
||
| Function: | |
Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U1. Two commitment complexes, CC1 and CC2, have been defined in yeast. CC1 is a basal complex dependent only on the 5' splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5' splice site region. This protein is involved in CC2 formation where it binds to the branchpoint binding protein MSL5, bridging the U1 snRNP-associated 5' splice site and the MSL5-associated branch point 3' intron splice site. {ECO:0000269|PubMed:10978320, ECO:0000269|PubMed:15020406, ECO:0000269|PubMed:9150140}. |
|
||
| Subunit: | |
Interacts with CRM1, MSL5, PRP8, and the RNA polymerase II largest subunit (RPB1). MSL5, MUD2 and PRP40 interact to form the commitment complex 2 (CC2), a precursor of mature spliceosomes. {ECO:0000269|PubMed:10978320, ECO:0000269|PubMed:15020406, ECO:0000269|PubMed:9150140}. |
| Subcellular location: | |
Nucleus {ECO:0000269|PubMed:15020406}. |
| Domain: | |
The WW and FF domains bind to the phosphorylated carboxy- terminal domain of RPB1. |
| Similarity: | |
Belongs to the PRPF40 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.