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UniProt functional annotation for Q9RGX8 | ||
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| UniProt code: Q9RGX8. |
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| Organism: | |
Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij). |
| Taxonomy: | |
Bacteria; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Zobellia. |
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| Function: | |
Cleaves the beta-1,4-linkages between beta-D-galactose and alpha-L-3,6-anhydro-galactose residues in agarose. Cleaves agarose in a random manner with retention of the anomeric-bond configuration, producing beta-anomers that give rise progressively to alpha-anomers when mutarotation takes place. Also tolerant to hybrid substrates containing C6-sulfate groups at the -4, +1, and +3 positions. {ECO:0000269|PubMed:15456406, ECO:0000269|PubMed:22778272}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.; EC=3.2.1.81; Evidence={ECO:0000269|PubMed:15456406, ECO:0000269|PubMed:22778272}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1 mM for agarose {ECO:0000269|PubMed:15456406}; Note=kcat is 100 sec(-1).; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:15456406, ECO:0000269|PubMed:22778272}. |
| Subcellular location: | |
Cell outer membrane {ECO:0000305|PubMed:15456406}; Lipid-anchor {ECO:0000305|PubMed:15456406}. |
| Induction: | |
When cells are grown with the low sulfated agar. {ECO:0000269|PubMed:22778272}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 16 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.